UniProtKB - P0ABG4 (FTSW_ECOLI)
Protein
Probable peptidoglycan glycosyltransferase FtsW
Gene
ftsW
Organism
Escherichia coli (strain K12)
Status
Functioni
Peptidoglycan polymerase that is essential for cell division (Probable). Functions probably in conjunction with the penicillin-binding protein 3 (ftsI) (PubMed:9603865, PubMed:11807049). Required for localization of FtsI (PubMed:11807049).2 Publications2 Publications
Caution
Has previously been proposed to be a lipid II flippase that transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane (PubMed:21386816, PubMed:24711460), but recent evidence suggests that FtsW may function as a peptidoglycan polymerase (PubMed:27643381). The identity of the lipid II flippase is still controversial with conflicting in vivo and in vitro results, but MurJ (AC P0AF16) is probably the lipid II flippase.Curated
Catalytic activityi
- [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H+UniRule annotationEC:2.4.1.129UniRule annotation
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
GO - Molecular functioni
- lipid-linked peptidoglycan transporter activity Source: EcoCyc
- peptidoglycan glycosyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell division Source: EcoliWiki
- cell wall organization Source: UniProtKB-KW
- FtsZ-dependent cytokinesis Source: UniProtKB-UniRule
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: EcoliWiki
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10344-MONOMER MetaCyc:EG10344-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
TCDBi | 2.A.103.1.1, the bacterial murein precursor exporter (mpe) family |
Names & Taxonomyi
Protein namesi | Recommended name: Probable peptidoglycan glycosyltransferase FtsWUniRule annotationBy similarity (EC:2.4.1.129UniRule annotationBy similarity)Short name: PGTUniRule annotationBy similarity Alternative name(s): Cell division protein FtsWUniRule annotationCurated Cell wall polymeraseUniRule annotationBy similarity Lipid II flippase FtsWCurated Peptidoglycan polymeraseUniRule annotationBy similarity Short name: PG polymeraseUniRule annotationBy similarity |
Gene namesi | Name:ftsWUniRule annotation Ordered Locus Names:b0089, JW0087 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation3 Publications; Multi-pass membrane protein UniRule annotation3 Publications
Note: Localizes to the division septum. Localization requires FtsZ, FtsA, FtsQ and FtsL, but not FtsI.
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- cell division site Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 13 – 33 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 34 – 47 | PeriplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 48 – 68 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 69 – 86 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 87 – 107 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 108 – 111 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 112 – 132 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 133 – 174 | CytoplasmicSequence analysisAdd BLAST | 42 | |
Transmembranei | 175 – 194 | HelicalUniRule annotationAdd BLAST | 20 | |
Topological domaini | 195 – 197 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 198 – 217 | HelicalUniRule annotationAdd BLAST | 20 | |
Topological domaini | 218 – 221 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 222 – 244 | HelicalUniRule annotationAdd BLAST | 23 | |
Topological domaini | 245 – 301 | PeriplasmicSequence analysisAdd BLAST | 57 | |
Transmembranei | 302 – 322 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 323 – 342 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 343 – 363 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 364 – 373 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 374 – 394 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 395 – 414 | CytoplasmicSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 145 | R → A: Loss of flippase activity. Does not affect localization at the division site. 1 Publication | 1 | |
Mutagenesisi | 150 – 152 | ELT → AAA: Does not affect flippase activity. 1 Publication | 3 | |
Mutagenesisi | 153 | K → N: Loss of flippase activity. Does not affect localization at the division site. 1 Publication | 1 | |
Mutagenesisi | 154 – 156 | LSL → AAA: Does not affect flippase activity. 1 Publication | 3 | |
Mutagenesisi | 170 | E → K in ftsW201; TS; blocks division at the initiation stage. | 1 | |
Mutagenesisi | 172 | R → S: Does not affect localization, but affects its thermosensitivity. 1 Publication | 1 | |
Mutagenesisi | 181 | P → L in ftsW1640; TS; blocks division at a late stage. | 1 | |
Mutagenesisi | 243 | R → Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 253 | P → L in ftsW263; TS; blocks division at the initiation stage. | 1 | |
Mutagenesisi | 311 | G → D: Thermosensitive. Fails to localize to the division site at 42 degrees Celsius. 1 Publication | 1 | |
Mutagenesisi | 368 | P → A: Cannot recruit FtsI at the division site; when associated with A-375. 1 Publication | 1 | |
Mutagenesisi | 375 | P → A: Cannot recruit FtsI at the division site; when associated with A-368. 1 Publication | 1 | |
Mutagenesisi | 402 | R → Q: Does not affect localization and activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000062700 | 1 – 414 | Probable peptidoglycan glycosyltransferase FtsWAdd BLAST | 414 |
Proteomic databases
jPOSTi | P0ABG4 |
PaxDbi | P0ABG4 |
PRIDEi | P0ABG4 |
Interactioni
Subunit structurei
Forms a complex with FtsI.
Interacts also with FtsL, FtsQ and FtsN.
4 PublicationsBinary interactionsi
Hide detailsP0ABG4
With | #Exp. | IntAct |
---|---|---|
ftsI [P0AD68] | 7 | EBI-1214767,EBI-548564 |
ftsN [P29131] | 3 | EBI-1214767,EBI-1134233 |
ftsQ [P06136] | 4 | EBI-1214767,EBI-1130157 |
Protein-protein interaction databases
BioGRIDi | 4261096, 505 interactors |
ComplexPortali | CPX-1936, Divisome complex |
DIPi | DIP-47989N |
IntActi | P0ABG4, 14 interactors |
MINTi | P0ABG4 |
STRINGi | 511145.b0089 |
Family & Domainsi
Domaini
The periplasmic loop between transmembrane domains 7 and 8 is essential for activity and the loop between transmembrane domains 9 and 10 is involved in the recruitment of FtsI at the division site.1 Publication
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0772, Bacteria |
HOGENOMi | CLU_029243_1_1_6 |
InParanoidi | P0ABG4 |
PhylomeDBi | P0ABG4 |
Family and domain databases
HAMAPi | MF_00913, PGT_FtsW_proteobact, 1 hit |
InterProi | View protein in InterPro IPR018365, Cell_cycle_FtsW-rel_CS IPR013437, FtsW IPR001182, FtsW/RodA |
PANTHERi | PTHR30474, PTHR30474, 1 hit |
Pfami | View protein in Pfam PF01098, FTSW_RODA_SPOVE, 1 hit |
TIGRFAMsi | TIGR02614, ftsW, 1 hit |
PROSITEi | View protein in PROSITE PS00428, FTSW_RODA_SPOVE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABG4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLSLPRLKM PRLPGFSILV WISTALKGWV MGSREKDTDS LIMYDRTLLW
60 70 80 90 100
LTFGLAAIGF IMVTSASMPI GQRLTNDPFF FAKRDGVYLI LAFILAIITL
110 120 130 140 150
RLPMEFWQRY SATMLLGSII LLMIVLVVGS SVKGASRWID LGLLRIQPAE
160 170 180 190 200
LTKLSLFCYI ANYLVRKGDE VRNNLRGFLK PMGVILVLAV LLLAQPDLGT
210 220 230 240 250
VVVLFVTTLA MLFLAGAKLW QFIAIIGMGI SAVVLLILAE PYRIRRVTAF
260 270 280 290 300
WNPWEDPFGS GYQLTQSLMA FGRGELWGQG LGNSVQKLEY LPEAHTDFIF
310 320 330 340 350
AIIGEELGYV GVVLALLMVF FVAFRAMSIG RKALEIDHRF SGFLACSIGI
360 370 380 390 400
WFSFQALVNV GAAAGMLPTK GLTLPLISYG GSSLLIMSTA IMMLLRIDYE
410
TRLEKAQAFV RGSR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30807 Genomic DNA Translation: AAA83859.1 X55034 Genomic DNA Translation: CAA38866.1 U00096 Genomic DNA Translation: AAC73200.1 AP009048 Genomic DNA Translation: BAB96657.1 X52644 Genomic DNA Translation: CAA36866.1 |
PIRi | A32581, CEECFW |
RefSeqi | NP_414631.1, NC_000913.3 WP_001295532.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73200; AAC73200; b0089 BAB96657; BAB96657; BAB96657 |
GeneIDi | 57730890 946322 |
KEGGi | ecj:JW0087 eco:b0089 |
PATRICi | fig|1411691.4.peg.2191 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30807 Genomic DNA Translation: AAA83859.1 X55034 Genomic DNA Translation: CAA38866.1 U00096 Genomic DNA Translation: AAC73200.1 AP009048 Genomic DNA Translation: BAB96657.1 X52644 Genomic DNA Translation: CAA36866.1 |
PIRi | A32581, CEECFW |
RefSeqi | NP_414631.1, NC_000913.3 WP_001295532.1, NZ_STEB01000010.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261096, 505 interactors |
ComplexPortali | CPX-1936, Divisome complex |
DIPi | DIP-47989N |
IntActi | P0ABG4, 14 interactors |
MINTi | P0ABG4 |
STRINGi | 511145.b0089 |
Protein family/group databases
TCDBi | 2.A.103.1.1, the bacterial murein precursor exporter (mpe) family |
Proteomic databases
jPOSTi | P0ABG4 |
PaxDbi | P0ABG4 |
PRIDEi | P0ABG4 |
Genome annotation databases
EnsemblBacteriai | AAC73200; AAC73200; b0089 BAB96657; BAB96657; BAB96657 |
GeneIDi | 57730890 946322 |
KEGGi | ecj:JW0087 eco:b0089 |
PATRICi | fig|1411691.4.peg.2191 |
Organism-specific databases
EchoBASEi | EB0340 |
Phylogenomic databases
eggNOGi | COG0772, Bacteria |
HOGENOMi | CLU_029243_1_1_6 |
InParanoidi | P0ABG4 |
PhylomeDBi | P0ABG4 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10344-MONOMER MetaCyc:EG10344-MONOMER |
Miscellaneous databases
PROi | PR:P0ABG4 |
Family and domain databases
HAMAPi | MF_00913, PGT_FtsW_proteobact, 1 hit |
InterProi | View protein in InterPro IPR018365, Cell_cycle_FtsW-rel_CS IPR013437, FtsW IPR001182, FtsW/RodA |
PANTHERi | PTHR30474, PTHR30474, 1 hit |
Pfami | View protein in Pfam PF01098, FTSW_RODA_SPOVE, 1 hit |
TIGRFAMsi | TIGR02614, ftsW, 1 hit |
PROSITEi | View protein in PROSITE PS00428, FTSW_RODA_SPOVE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FTSW_ECOLI | |
Accessioni | P0ABG4Primary (citable) accession number: P0ABG4 Secondary accession number(s): P16457 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | April 7, 2021 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families