UniProtKB - P0ABF8 (PGSA_ECOLI)
Protein
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Gene
pgsA
Organism
Escherichia coli (strain K12)
Status
Functioni
This protein catalyzes the committed step to the synthesis of the acidic phospholipids.
Catalytic activityi
- a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = 1,2-diacyl-sn-glycero-3-phospho-(1ʼ-sn-glycero-3ʼ-phosphate) + CMP + H+EC:2.7.8.5
: phosphatidylglycerol biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
- Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase B (pgpB), Phosphatidylglycerophosphatase C (pgpC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
GO - Biological processi
- glycerophospholipid biosynthetic process Source: GO_Central
- phosphatidylglycerol biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:PHOSPHAGLYPSYN-MONOMER MetaCyc:PHOSPHAGLYPSYN-MONOMER |
UniPathwayi | UPA00084;UER00503 |
Names & Taxonomyi
Protein namesi | Recommended name: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC:2.7.8.5)Alternative name(s): Phosphatidylglycerophosphate synthase Short name: PGP synthase |
Gene namesi | Name:pgsA Ordered Locus Names:b1912, JW1897 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 13 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 14 – 38 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 39 – 61 | PeriplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 62 – 82 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 83 – 87 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 88 – 108 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 109 – 146 | PeriplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 147 – 169 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 170 – 182 | CytoplasmicSequence analysisAdd BLAST | 13 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 60 | T → P in pgsA3; lower activity. | 1 | |
Mutagenesisi | 92 | T → I in pgsA10; lower activity. | 1 |
Chemistry databases
ChEMBLi | CHEMBL3309009 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCurated | |||
ChainiPRO_0000056773 | 2 – 182 | CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferaseAdd BLAST | 181 |
Proteomic databases
jPOSTi | P0ABF8 |
PaxDbi | P0ABF8 |
PRIDEi | P0ABF8 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261587, 267 interactors |
IntActi | P0ABF8, 1 interactor |
STRINGi | 511145.b1912 |
Family & Domainsi
Sequence similaritiesi
Belongs to the CDP-alcohol phosphatidyltransferase class-I family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0558, Bacteria |
InParanoidi | P0ABF8 |
PhylomeDBi | P0ABF8 |
Family and domain databases
Gene3Di | 1.20.120.1760, 1 hit |
HAMAPi | MF_01437, PgsA, 1 hit |
InterProi | View protein in InterPro IPR000462, CDP-OH_P_trans IPR043130, CDP-OH_PTrfase_TM_dom IPR023762, PGP_synthase_bac IPR004570, Phosphatidylglycerol_P_synth |
Pfami | View protein in Pfam PF01066, CDP-OH_P_transf, 1 hit |
PIRSFi | PIRSF000847, Phos_ph_gly_syn, 1 hit |
TIGRFAMsi | TIGR00560, pgsA, 1 hit |
PROSITEi | View protein in PROSITE PS00379, CDP_ALCOHOL_P_TRANSF, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ABF8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQFNIPTLLT LFRVILIPFF VLVFYLPVTW SPFAAALIFC VAAVTDWFDG
60 70 80 90 100
FLARRWNQST RFGAFLDPVA DKVLVAIAMV LVTEHYHSWW VTLPAATMIA
110 120 130 140 150
REIIISALRE WMAELGKRSS VAVSWIGKVK TTAQMVALAW LLWRPNIWVE
160 170 180
YAGIALFFVA AVLTLWSMLQ YLSAARADLL DQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12299 Genomic DNA Translation: AAA98754.1 U00096 Genomic DNA Translation: AAC74979.1 AP009048 Genomic DNA Translation: BAA15732.1 |
PIRi | E64954, XNECPG |
RefSeqi | NP_416422.1, NC_000913.3 WP_001160187.1, NZ_STEB01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC74979; AAC74979; b1912 BAA15732; BAA15732; BAA15732 |
GeneIDi | 58461784 945791 |
KEGGi | ecj:JW1897 eco:b1912 |
PATRICi | fig|1411691.4.peg.338 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12299 Genomic DNA Translation: AAA98754.1 U00096 Genomic DNA Translation: AAC74979.1 AP009048 Genomic DNA Translation: BAA15732.1 |
PIRi | E64954, XNECPG |
RefSeqi | NP_416422.1, NC_000913.3 WP_001160187.1, NZ_STEB01000026.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261587, 267 interactors |
IntActi | P0ABF8, 1 interactor |
STRINGi | 511145.b1912 |
Chemistry databases
ChEMBLi | CHEMBL3309009 |
Proteomic databases
jPOSTi | P0ABF8 |
PaxDbi | P0ABF8 |
PRIDEi | P0ABF8 |
Genome annotation databases
EnsemblBacteriai | AAC74979; AAC74979; b1912 BAA15732; BAA15732; BAA15732 |
GeneIDi | 58461784 945791 |
KEGGi | ecj:JW1897 eco:b1912 |
PATRICi | fig|1411691.4.peg.338 |
Organism-specific databases
EchoBASEi | EB0700 |
Phylogenomic databases
eggNOGi | COG0558, Bacteria |
InParanoidi | P0ABF8 |
PhylomeDBi | P0ABF8 |
Enzyme and pathway databases
UniPathwayi | UPA00084;UER00503 |
BioCyci | EcoCyc:PHOSPHAGLYPSYN-MONOMER MetaCyc:PHOSPHAGLYPSYN-MONOMER |
Miscellaneous databases
PROi | PR:P0ABF8 |
Family and domain databases
Gene3Di | 1.20.120.1760, 1 hit |
HAMAPi | MF_01437, PgsA, 1 hit |
InterProi | View protein in InterPro IPR000462, CDP-OH_P_trans IPR043130, CDP-OH_PTrfase_TM_dom IPR023762, PGP_synthase_bac IPR004570, Phosphatidylglycerol_P_synth |
Pfami | View protein in Pfam PF01066, CDP-OH_P_transf, 1 hit |
PIRSFi | PIRSF000847, Phos_ph_gly_syn, 1 hit |
TIGRFAMsi | TIGR00560, pgsA, 1 hit |
PROSITEi | View protein in PROSITE PS00379, CDP_ALCOHOL_P_TRANSF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PGSA_ECOLI | |
Accessioni | P0ABF8Primary (citable) accession number: P0ABF8 Secondary accession number(s): P06978 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families