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UniProtKB - P0ABF6 (CDD_ECOLI)
Protein
Cytidine deaminase
Gene
cdd
Organism
Escherichia coli (strain K12)
Status
Functioni
This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
Catalytic activityi
Cofactori
Zn2+3 PublicationsNote: Binds 1 zinc ion.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 102 | Zinc; catalytic3 Publications | 1 | |
Active sitei | 104 | Proton donor3 Publications | 1 | |
Metal bindingi | 129 | Zinc; catalytic3 Publications | 1 | |
Metal bindingi | 132 | Zinc; catalytic3 Publications | 1 |
GO - Molecular functioni
- cytidine deaminase activity Source: EcoCyc
- deoxycytidine deaminase activity Source: EcoCyc
- identical protein binding Source: IntAct
- pyrimidine nucleoside binding Source: EcoliWiki
- zinc ion binding Source: EcoCyc
GO - Biological processi
- cytidine deamination Source: EcoCyc
- deoxycytidine catabolic process Source: EcoCyc
- nucleobase-containing small molecule interconversion Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:CYTIDEAM-MONOMER |
BRENDAi | 3.5.4.5, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytidine deaminaseUniRule annotation (EC:3.5.4.5UniRule annotation)Alternative name(s): Cytidine aminohydrolaseUniRule annotation Short name: CDAUniRule annotation |
Gene namesi | Name:cddUniRule annotation Ordered Locus Names:b2143, JW2131 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171649 | 1 – 294 | Cytidine deaminaseAdd BLAST | 294 |
Proteomic databases
jPOSTi | P0ABF6 |
PaxDbi | P0ABF6 |
PRIDEi | P0ABF6 |
2D gel databases
SWISS-2DPAGEi | P0ABF6 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation3 PublicationsBinary interactionsi
P0ABF6
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-550703,EBI-550703 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4263038, 10 interactors 851005, 2 interactors |
DIPi | DIP-36169N |
IntActi | P0ABF6, 2 interactors |
STRINGi | 511145.b2143 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABF6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABF6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 48 – 168 | CMP/dCMP-type deaminase 1PROSITE-ProRule annotationAdd BLAST | 121 | |
Domaini | 186 – 294 | CMP/dCMP-type deaminase 2PROSITE-ProRule annotationAdd BLAST | 109 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 89 – 91 | Substrate binding3 Publications | 3 |
Domaini
Each monomer is composed of a small N-terminal alpha-helical domain and two larger core domains that have nearly identical tertiary structures and are related by approximate two-fold symmetry, but lack homology.
Sequence similaritiesi
Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0295, Bacteria |
HOGENOMi | CLU_052424_0_0_6 |
InParanoidi | P0ABF6 |
PhylomeDBi | P0ABF6 |
Family and domain databases
HAMAPi | MF_01558, Cyt_deam, 1 hit |
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR013171, Cyd/dCyd_deaminase_Zn-bd IPR006263, Cyt_deam_dimer IPR016193, Cytidine_deaminase-like IPR020797, Cytidine_deaminase_bacteria |
Pfami | View protein in Pfam PF00383, dCMP_cyt_deam_1, 1 hit PF08211, dCMP_cyt_deam_2, 1 hit |
SUPFAMi | SSF53927, SSF53927, 2 hits |
TIGRFAMsi | TIGR01355, cyt_deam_dimer, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P0ABF6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHPRFQTAFA QLADNLQSAL EPILADKYFP ALLTGEQVSS LKSATGLDED
60 70 80 90 100
ALAFALLPLA AACARTPLSN FNVGAIARGV SGTWYFGANM EFIGATMQQT
110 120 130 140 150
VHAEQSAISH AWLSGEKALA AITVNYTPCG HCRQFMNELN SGLDLRIHLP
160 170 180 190 200
GREAHALRDY LPDAFGPKDL EIKTLLMDEQ DHGYALTGDA LSQAAIAAAN
210 220 230 240 250
RSHMPYSKSP SGVALECKDG RIFSGSYAEN AAFNPTLPPL QGALILLNLK
260 270 280 290
GYDYPDIQRA VLAEKADAPL IQWDATSATL KALGCHSIDR VLLA
Sequence cautioni
The sequence CAA44849 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | R → I AA sequence (PubMed:8899705).Curated | 1 | |
Sequence conflicti | 58 – 77 | PLAAA…VGAIA → RWRRPVRVRHCRILMLAQLR (PubMed:2575702).CuratedAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M60916 Genomic DNA Translation: AAA23542.1 X63144 Genomic DNA Translation: CAA44849.1 Frameshift. U00007 Genomic DNA Translation: AAA60533.1 U00096 Genomic DNA Translation: AAC75204.1 AP009048 Genomic DNA Translation: BAE76620.1 X16419 Genomic DNA Translation: CAA34441.1 Z47804 Genomic DNA Translation: CAA87765.1 |
PIRi | F64982 |
RefSeqi | NP_416648.1, NC_000913.3 WP_000553555.1, NZ_SSZK01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75204; AAC75204; b2143 BAE76620; BAE76620; BAE76620 |
GeneIDi | 58388962 946663 |
KEGGi | ecj:JW2131 eco:b2143 |
PATRICi | fig|1411691.4.peg.99 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M60916 Genomic DNA Translation: AAA23542.1 X63144 Genomic DNA Translation: CAA44849.1 Frameshift. U00007 Genomic DNA Translation: AAA60533.1 U00096 Genomic DNA Translation: AAC75204.1 AP009048 Genomic DNA Translation: BAE76620.1 X16419 Genomic DNA Translation: CAA34441.1 Z47804 Genomic DNA Translation: CAA87765.1 |
PIRi | F64982 |
RefSeqi | NP_416648.1, NC_000913.3 WP_000553555.1, NZ_SSZK01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AF2 | X-ray | 2.30 | A | 1-294 | [»] | |
1ALN | X-ray | 2.30 | A | 1-294 | [»] | |
1CTT | X-ray | 2.20 | A | 1-294 | [»] | |
1CTU | X-ray | 2.30 | A | 1-294 | [»] | |
SMRi | P0ABF6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263038, 10 interactors 851005, 2 interactors |
DIPi | DIP-36169N |
IntActi | P0ABF6, 2 interactors |
STRINGi | 511145.b2143 |
Chemistry databases
DrugBanki | DB04385, 3-Deazacytidine DB03068, Zebularine |
2D gel databases
SWISS-2DPAGEi | P0ABF6 |
Proteomic databases
jPOSTi | P0ABF6 |
PaxDbi | P0ABF6 |
PRIDEi | P0ABF6 |
Genome annotation databases
EnsemblBacteriai | AAC75204; AAC75204; b2143 BAE76620; BAE76620; BAE76620 |
GeneIDi | 58388962 946663 |
KEGGi | ecj:JW2131 eco:b2143 |
PATRICi | fig|1411691.4.peg.99 |
Organism-specific databases
EchoBASEi | EB0135 |
Phylogenomic databases
eggNOGi | COG0295, Bacteria |
HOGENOMi | CLU_052424_0_0_6 |
InParanoidi | P0ABF6 |
PhylomeDBi | P0ABF6 |
Enzyme and pathway databases
BioCyci | EcoCyc:CYTIDEAM-MONOMER |
BRENDAi | 3.5.4.5, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0ABF6 |
PROi | PR:P0ABF6 |
Family and domain databases
HAMAPi | MF_01558, Cyt_deam, 1 hit |
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR013171, Cyd/dCyd_deaminase_Zn-bd IPR006263, Cyt_deam_dimer IPR016193, Cytidine_deaminase-like IPR020797, Cytidine_deaminase_bacteria |
Pfami | View protein in Pfam PF00383, dCMP_cyt_deam_1, 1 hit PF08211, dCMP_cyt_deam_2, 1 hit |
SUPFAMi | SSF53927, SSF53927, 2 hits |
TIGRFAMsi | TIGR01355, cyt_deam_dimer, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CDD_ECOLI | |
Accessioni | P0ABF6Primary (citable) accession number: P0ABF6 Secondary accession number(s): P13652, Q2MAT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families