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Protein

Poly(A) polymerase I

Gene

pcnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.UniRule annotation3 Publications

Miscellaneous

Uses the non-canonical initiation codon AUU, which limits pcnB expression.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80UniRule annotation1
Active sitei82UniRule annotation1
Active sitei162UniRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-UniRule
  • polynucleotide adenylyltransferase activity Source: EcoliWiki
  • RNA binding Source: EcoliWiki

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing, Plasmid copy control, Transcription
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10690-MONOMER
MetaCyc:EG10690-MONOMER
BRENDAi2.7.7.19 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase IUniRule annotation (EC:2.7.7.19UniRule annotation)
Short name:
PAP IUniRule annotation
Alternative name(s):
Plasmid copy number protein
Gene namesi
Name:pcnBUniRule annotation
Ordered Locus Names:b0143, JW5808
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10690 pcnB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00004111631 – 101 Publication10
ChainiPRO_000013909011 – 465Poly(A) polymerase IAdd BLAST455

Proteomic databases

PaxDbiP0ABF1
PRIDEiP0ABF1

Expressioni

Inductioni

Transcription regulation of pcnB is a complex process that involves several factors such as Sigma-70 (rpoD), Sigma-S (rpoS), ppGpp and DksA.1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with CsdA, RhlE and SrmB.1 Publication

Protein-protein interaction databases

BioGridi4263029, 32 interactors
DIPiDIP-47859N
IntActiP0ABF1, 12 interactors
STRINGi316385.ECDH10B_0123

Structurei

3D structure databases

ProteinModelPortaliP0ABF1
SMRiP0ABF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ0 Bacteria
COG0617 LUCA
HOGENOMiHOG000256527
InParanoidiP0ABF1
KOiK00970
PhylomeDBiP0ABF1

Family and domain databases

CDDicd05398 NT_ClassII-CCAase, 1 hit
HAMAPiMF_00957 PolyA_pol, 1 hit
InterProiView protein in InterPro
IPR002646 PolA_pol_head_dom
IPR010206 PolA_pol_I
IPR025866 PolyA_pol_arg_C_dom
IPR032828 PolyA_RNA-bd
PfamiView protein in Pfam
PF01743 PolyA_pol, 1 hit
PF12626 PolyA_pol_arg_C, 1 hit
PF12627 PolyA_pol_RNAbd, 1 hit
TIGRFAMsiTIGR01942 pcnB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABF1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK
60 70 80 90 100
VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR
110 120 130 140 150
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI
160 170 180 190 200
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET
210 220 230 240 250
RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES
260 270 280 290 300
LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK
310 320 330 340 350
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN
360 370 380 390 400
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA
410 420 430 440 450
YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR
460
TRRPRKRAPR REGTA
Length:465
Mass (Da):53,871
Last modified:September 19, 2006 - v2
Checksum:i0FAE30F2F95FB76E
GO

Sequence cautioni

The sequence AAA24303 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAB24139 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93R → A in AAA24303 (PubMed:2537812).Curated1
Sequence conflicti315A → T in AAA24303 (PubMed:2537812).Curated1
Sequence conflicti332K → N in AAA24303 (PubMed:2537812).Curated1
Sequence conflicti437 – 438ML → IV in AAA24303 (PubMed:2537812).Curated2
Sequence conflicti449 – 465RRTRR…REGTA → QSYSSSTQTRTTS in AAA24303 (PubMed:2537812).CuratedAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20574 Genomic DNA Translation: AAA24303.1 Different initiation.
U00096 Genomic DNA Translation: AAC73254.2
AP009048 Genomic DNA Translation: BAB96720.2
S48039 Genomic DNA Translation: AAB24139.1 Different initiation.
PIRiG64737
RefSeqiNP_414685.4, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC73254; AAC73254; b0143
BAB96720; BAB96720; BAB96720
GeneIDi947318
KEGGiecj:JW5808
eco:b0143
PATRICifig|511145.12.peg.148

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20574 Genomic DNA Translation: AAA24303.1 Different initiation.
U00096 Genomic DNA Translation: AAC73254.2
AP009048 Genomic DNA Translation: BAB96720.2
S48039 Genomic DNA Translation: AAB24139.1 Different initiation.
PIRiG64737
RefSeqiNP_414685.4, NC_000913.3

3D structure databases

ProteinModelPortaliP0ABF1
SMRiP0ABF1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263029, 32 interactors
DIPiDIP-47859N
IntActiP0ABF1, 12 interactors
STRINGi316385.ECDH10B_0123

Proteomic databases

PaxDbiP0ABF1
PRIDEiP0ABF1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73254; AAC73254; b0143
BAB96720; BAB96720; BAB96720
GeneIDi947318
KEGGiecj:JW5808
eco:b0143
PATRICifig|511145.12.peg.148

Organism-specific databases

EchoBASEiEB0684
EcoGeneiEG10690 pcnB

Phylogenomic databases

eggNOGiENOG4105DJ0 Bacteria
COG0617 LUCA
HOGENOMiHOG000256527
InParanoidiP0ABF1
KOiK00970
PhylomeDBiP0ABF1

Enzyme and pathway databases

BioCyciEcoCyc:EG10690-MONOMER
MetaCyc:EG10690-MONOMER
BRENDAi2.7.7.19 2026

Miscellaneous databases

PROiPR:P0ABF1

Family and domain databases

CDDicd05398 NT_ClassII-CCAase, 1 hit
HAMAPiMF_00957 PolyA_pol, 1 hit
InterProiView protein in InterPro
IPR002646 PolA_pol_head_dom
IPR010206 PolA_pol_I
IPR025866 PolyA_pol_arg_C_dom
IPR032828 PolyA_RNA-bd
PfamiView protein in Pfam
PF01743 PolyA_pol, 1 hit
PF12626 PolyA_pol_arg_C, 1 hit
PF12627 PolyA_pol_RNAbd, 1 hit
TIGRFAMsiTIGR01942 pcnB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCNB_ECOLI
AccessioniPrimary (citable) accession number: P0ABF1
Secondary accession number(s): P13685, P78050, Q8X910
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: November 7, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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