UniProtKB - P0ABE9 (CYNT_ECOLI)
Protein
Carbonic anhydrase 1
Gene
cynT
Organism
Escherichia coli (strain K12)
Status
Functioni
Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to catalyze the hydration of carbon dioxide and thus prevent depletion of cellular bicarbonate.1 Publication
Catalytic activityi
- EC:4.2.1.11 Publication
Cofactori
Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 39 | ZincBy similarity | 1 | |
Metal bindingi | 41 | ZincBy similarity | 1 | |
Metal bindingi | 98 | ZincBy similarity | 1 | |
Metal bindingi | 101 | ZincBy similarity | 1 |
GO - Molecular functioni
- carbonate dehydratase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- carbon utilization Source: InterPro
- cyanate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:CARBODEHYDRAT-MONOMER MetaCyc:CARBODEHYDRAT-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Carbonic anhydrase 1 (EC:4.2.1.11 Publication)Alternative name(s): Carbonate dehydratase 1 |
Gene namesi | Name:cynT1 Publication Ordered Locus Names:b0339, JW0330 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000077459 | 1 – 219 | Carbonic anhydrase 1Add BLAST | 219 |
Proteomic databases
PaxDbi | P0ABE9 |
PRIDEi | P0ABE9 |
Expressioni
Inductioni
By cyanate.1 Publication
Interactioni
Subunit structurei
Oligomer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259815, 4 interactors |
DIPi | DIP-47965N |
IntActi | P0ABE9, 1 interactor |
STRINGi | 511145.b0339 |
Family & Domainsi
Sequence similaritiesi
Belongs to the beta-class carbonic anhydrase family.Curated
Phylogenomic databases
eggNOGi | COG0288, Bacteria |
HOGENOMi | CLU_053879_5_3_6 |
InParanoidi | P0ABE9 |
PhylomeDBi | P0ABE9 |
Family and domain databases
Gene3Di | 3.40.1050.10, 1 hit |
InterProi | View protein in InterPro IPR001765, Carbonic_anhydrase IPR015892, Carbonic_anhydrase_CS IPR036874, Carbonic_anhydrase_sf |
Pfami | View protein in Pfam PF00484, Pro_CA, 1 hit |
SMARTi | View protein in SMART SM00947, Pro_CA, 1 hit |
SUPFAMi | SSF53056, SSF53056, 1 hit |
PROSITEi | View protein in PROSITE PS00704, PROK_CO2_ANHYDRASE_1, 1 hit PS00705, PROK_CO2_ANHYDRASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABE9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKEIIDGFLK FQREAFPKRE ALFKQLATQQ SPRTLFISCS DSRLVPELVT
60 70 80 90 100
QREPGDLFVI RNAGNIVPSY GPEPGGVSAS VEYAVAALRV SDIVICGHSN
110 120 130 140 150
CGAMTAIASC QCMDHMPAVS HWLRYADSAR VVNEARPHSD LPSKAAAMVR
160 170 180 190 200
ENVIAQLANL QTHPSVRLAL EEGRIALHGW VYDIESGSIA AFDGATRQFV
210
PLAANPRVCA IPLRQPTAA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 174 – 176 | RIA → GS in AAA23625 (PubMed:3049588).Curated | 3 | |
Sequence conflicti | 212 | P → R in AAA23625 (PubMed:3049588).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23219 Genomic DNA Translation: AAA23625.1 U73857 Genomic DNA Translation: AAB18063.1 U00096 Genomic DNA Translation: AAC73442.1 AP009048 Genomic DNA Translation: BAE76121.1 |
PIRi | C64761, QRECTC |
RefSeqi | NP_414873.1, NC_000913.3 WP_000658652.1, NZ_STEB01000036.1 |
Genome annotation databases
EnsemblBacteriai | AAC73442; AAC73442; b0339 BAE76121; BAE76121; BAE76121 |
GeneIDi | 57731524 946548 |
KEGGi | ecj:JW0330 eco:b0339 |
PATRICi | fig|1411691.4.peg.1938 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23219 Genomic DNA Translation: AAA23625.1 U73857 Genomic DNA Translation: AAB18063.1 U00096 Genomic DNA Translation: AAC73442.1 AP009048 Genomic DNA Translation: BAE76121.1 |
PIRi | C64761, QRECTC |
RefSeqi | NP_414873.1, NC_000913.3 WP_000658652.1, NZ_STEB01000036.1 |
3D structure databases
SMRi | P0ABE9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259815, 4 interactors |
DIPi | DIP-47965N |
IntActi | P0ABE9, 1 interactor |
STRINGi | 511145.b0339 |
Proteomic databases
PaxDbi | P0ABE9 |
PRIDEi | P0ABE9 |
Genome annotation databases
EnsemblBacteriai | AAC73442; AAC73442; b0339 BAE76121; BAE76121; BAE76121 |
GeneIDi | 57731524 946548 |
KEGGi | ecj:JW0330 eco:b0339 |
PATRICi | fig|1411691.4.peg.1938 |
Organism-specific databases
EchoBASEi | EB0173 |
Phylogenomic databases
eggNOGi | COG0288, Bacteria |
HOGENOMi | CLU_053879_5_3_6 |
InParanoidi | P0ABE9 |
PhylomeDBi | P0ABE9 |
Enzyme and pathway databases
BioCyci | EcoCyc:CARBODEHYDRAT-MONOMER MetaCyc:CARBODEHYDRAT-MONOMER |
Miscellaneous databases
PROi | PR:P0ABE9 |
Family and domain databases
Gene3Di | 3.40.1050.10, 1 hit |
InterProi | View protein in InterPro IPR001765, Carbonic_anhydrase IPR015892, Carbonic_anhydrase_CS IPR036874, Carbonic_anhydrase_sf |
Pfami | View protein in Pfam PF00484, Pro_CA, 1 hit |
SMARTi | View protein in SMART SM00947, Pro_CA, 1 hit |
SUPFAMi | SSF53056, SSF53056, 1 hit |
PROSITEi | View protein in PROSITE PS00704, PROK_CO2_ANHYDRASE_1, 1 hit PS00705, PROK_CO2_ANHYDRASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYNT_ECOLI | |
Accessioni | P0ABE9Primary (citable) accession number: P0ABE9 Secondary accession number(s): P17582, P78278, Q2MC85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | February 10, 2021 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families