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UniProtKB - P0ABD5 (ACCA_ECOLI)
Protein
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Gene
accA
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO2 group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.
UniRule annotation1 PublicationCatalytic activityi
- acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N6-biotinyl-L-lysyl-[protein]UniRule annotation1 PublicationEC:2.1.3.15UniRule annotation1 Publication
Activity regulationi
Competitively inhibited by pyrrolidine dione antibiotic moiramide B (CPD1).1 Publication
Kineticsi
- KM=100 µM for malonyl-CoA1 Publication
- KM=10 mM for biocytin1 Publication
: malonyl-CoA biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes malonyl-CoA from acetyl-CoA.UniRule annotation This subpathway is part of the pathway malonyl-CoA biosynthesis, which is itself part of Lipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes malonyl-CoA from acetyl-CoA, the pathway malonyl-CoA biosynthesis and in Lipid metabolism.
GO - Molecular functioni
- acetyl-CoA carboxylase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- carboxyl- or carbamoyltransferase activity Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
GO - Biological processi
- fatty acid biosynthetic process Source: ComplexPortal
- long-chain fatty acid biosynthetic process Source: EcoliWiki
- malonyl-CoA biosynthetic process Source: ComplexPortal
Keywordsi
Molecular function | Transferase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:CARBOXYL-TRANSFERASE-ALPHA-MONOMER |
BRENDAi | 2.1.3.15, 2026 2.3.2.31, 2681 6.4.1.2, 2026 |
SABIO-RKi | P0ABD5 |
UniPathwayi | UPA00655;UER00711 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetyl-coenzyme A carboxylase carboxyl transferase subunit alphaUniRule annotation (EC:2.1.3.15UniRule annotation1 Publication)Short name: ACCase subunit alphaUniRule annotation Short name: Acetyl-CoA carboxylase carboxyltransferase subunit alphaUniRule annotation |
Gene namesi | Name:accAUniRule annotation Ordered Locus Names:b0185, JW0180 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Other locations
- acetate CoA-transferase complex Source: EcoCyc
- acetyl-CoA carboxylase complex Source: ComplexPortal
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000146774 | 2 – 319 | Acetyl-coenzyme A carboxylase carboxyl transferase subunit alphaAdd BLAST | 318 |
Proteomic databases
jPOSTi | P0ABD5 |
PaxDbi | P0ABD5 |
PRIDEi | P0ABD5 |
Interactioni
Subunit structurei
Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).
Binary interactionsi
P0ABD5
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-542031,EBI-542031 |
accD [P0A9Q5] | 17 | EBI-542031,EBI-542064 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259753, 211 interactors 849295, 5 interactors |
ComplexPortali | CPX-3206, Acetyl-CoA carboxylase complex |
DIPi | DIP-35897N |
IntActi | P0ABD5, 32 interactors |
MINTi | P0ABD5 |
STRINGi | 511145.b0185 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABD5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABD5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 296 | CoA carboxyltransferase C-terminalPROSITE-ProRule annotationAdd BLAST | 262 |
Sequence similaritiesi
Belongs to the AccA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0825, Bacteria |
HOGENOMi | CLU_015486_0_2_6 |
InParanoidi | P0ABD5 |
PhylomeDBi | P0ABD5 |
Family and domain databases
HAMAPi | MF_00823, AcetylCoA_CT_alpha, 1 hit |
InterProi | View protein in InterPro IPR001095, Acetyl_CoA_COase_a_su IPR029045, ClpP/crotonase-like_dom_sf IPR011763, COA_CT_C |
PANTHERi | PTHR42853, PTHR42853, 1 hit |
Pfami | View protein in Pfam PF03255, ACCA, 1 hit |
PRINTSi | PR01069, ACCCTRFRASEA |
SUPFAMi | SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR00513, accA, 1 hit |
PROSITEi | View protein in PROSITE PS50989, COA_CT_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ABD5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLNFLDFEQ PIAELEAKID SLTAVSRQDE KLDINIDEEV HRLREKSVEL
60 70 80 90 100
TRKIFADLGA WQIAQLARHP QRPYTLDYVR LAFDEFDELA GDRAYADDKA
110 120 130 140 150
IVGGIARLDG RPVMIIGHQK GRETKEKIRR NFGMPAPEGY RKALRLMQMA
160 170 180 190 200
ERFKMPIITF IDTPGAYPGV GAEERGQSEA IARNLREMSR LGVPVVCTVI
210 220 230 240 250
GEGGSGGALA IGVGDKVNML QYSTYSVISP EGCASILWKS ADKAPLAAEA
260 270 280 290 300
MGIIAPRLKE LKLIDSIIPE PLGGAHRNPE AMAASLKAQL LADLADLDVL
310
STEDLKNRRY QRLMSYGYA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96394 Genomic DNA Translation: AAA70370.1 D49445 Genomic DNA Translation: BAA08425.1 U70214 Genomic DNA Translation: AAB08614.1 U00096 Genomic DNA Translation: AAC73296.1 AP009048 Genomic DNA Translation: BAA77860.2 D87518 Genomic DNA Translation: BAA21655.1 M19334 Genomic DNA No translation available. |
PIRi | A43452 |
RefSeqi | NP_414727.1, NC_000913.3 WP_000055741.1, NZ_STEB01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73296; AAC73296; b0185 BAA77860; BAA77860; BAA77860 |
GeneIDi | 66671527 944895 |
KEGGi | ecj:JW0180 eco:b0185 |
PATRICi | fig|1411691.4.peg.2094 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96394 Genomic DNA Translation: AAA70370.1 D49445 Genomic DNA Translation: BAA08425.1 U70214 Genomic DNA Translation: AAB08614.1 U00096 Genomic DNA Translation: AAC73296.1 AP009048 Genomic DNA Translation: BAA77860.2 D87518 Genomic DNA Translation: BAA21655.1 M19334 Genomic DNA No translation available. |
PIRi | A43452 |
RefSeqi | NP_414727.1, NC_000913.3 WP_000055741.1, NZ_STEB01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2F9Y | X-ray | 3.20 | A | 1-319 | [»] | |
SMRi | P0ABD5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259753, 211 interactors 849295, 5 interactors |
ComplexPortali | CPX-3206, Acetyl-CoA carboxylase complex |
DIPi | DIP-35897N |
IntActi | P0ABD5, 32 interactors |
MINTi | P0ABD5 |
STRINGi | 511145.b0185 |
Proteomic databases
jPOSTi | P0ABD5 |
PaxDbi | P0ABD5 |
PRIDEi | P0ABD5 |
Genome annotation databases
EnsemblBacteriai | AAC73296; AAC73296; b0185 BAA77860; BAA77860; BAA77860 |
GeneIDi | 66671527 944895 |
KEGGi | ecj:JW0180 eco:b0185 |
PATRICi | fig|1411691.4.peg.2094 |
Organism-specific databases
EchoBASEi | EB1600 |
Phylogenomic databases
eggNOGi | COG0825, Bacteria |
HOGENOMi | CLU_015486_0_2_6 |
InParanoidi | P0ABD5 |
PhylomeDBi | P0ABD5 |
Enzyme and pathway databases
UniPathwayi | UPA00655;UER00711 |
BioCyci | EcoCyc:CARBOXYL-TRANSFERASE-ALPHA-MONOMER |
BRENDAi | 2.1.3.15, 2026 2.3.2.31, 2681 6.4.1.2, 2026 |
SABIO-RKi | P0ABD5 |
Miscellaneous databases
EvolutionaryTracei | P0ABD5 |
PROi | PR:P0ABD5 |
Family and domain databases
HAMAPi | MF_00823, AcetylCoA_CT_alpha, 1 hit |
InterProi | View protein in InterPro IPR001095, Acetyl_CoA_COase_a_su IPR029045, ClpP/crotonase-like_dom_sf IPR011763, COA_CT_C |
PANTHERi | PTHR42853, PTHR42853, 1 hit |
Pfami | View protein in Pfam PF03255, ACCA, 1 hit |
PRINTSi | PR01069, ACCCTRFRASEA |
SUPFAMi | SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR00513, accA, 1 hit |
PROSITEi | View protein in PROSITE PS50989, COA_CT_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACCA_ECOLI | |
Accessioni | P0ABD5Primary (citable) accession number: P0ABD5 Secondary accession number(s): P30867 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families