UniProtKB - P0ABC7 (HFLK_ECOLI)
Protein
Modulator of FtsH protease HflK
Gene
hflK
Organism
Escherichia coli (strain K12)
Status
Functioni
HflC and HflK help govern the stability of phage lambda cII protein, and thereby control the lysogenization frequency of phage lambda. HflKC inhibits the SecY-degrading activity of FtsH, possibly helping quality control of integral membrane proteins.1 Publication
Miscellaneous
Integration of this protein into the membrane depends on SecA, SecY and SecD but not on SecB or FtsY. HflK is unstable in the absence of HflC.
Caution
Was originally (PubMed:2973057) thought to be a protease. However, removal of residues '165-200' of complex member HflC (a ClpP-protease-like motif) does not alter the lysogenization process, and in vitro studies show no evidence of a protease activity for the isolated HflKC complex.1 Publication
GO - Biological processi
- negative regulation of catalytic activity Source: EcoCyc
- response to heat Source: EcoliWiki
Enzyme and pathway databases
BioCyci | EcoCyc:EG10436-MONOMER MetaCyc:EG10436-MONOMER |
Protein family/group databases
MEROPSi | I87.002 |
Names & Taxonomyi
Protein namesi | Recommended name: Modulator of FtsH protease HflK |
Gene namesi | Name:hflK Synonyms:hflA Ordered Locus Names:b4174, JW4132 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 79 | Cytoplasmic1 PublicationAdd BLAST | 79 | |
Transmembranei | 80 – 100 | Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST | 21 | |
Topological domaini | 101 – 419 | Periplasmic1 PublicationAdd BLAST | 319 |
GO - Cellular componenti
- cytosol Source: EcoCyc
- integral component of external side of plasma membrane Source: EcoCyc
- membrane protein complex Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 145 | A → V in hflK13; stabilizes overproduced SecY but not overproduced cII protein. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094085 | 1 – 419 | Modulator of FtsH protease HflKAdd BLAST | 419 |
Proteomic databases
jPOSTi | P0ABC7 |
PaxDbi | P0ABC7 |
PRIDEi | P0ABC7 |
Interactioni
Subunit structurei
HflC and HflK interact to form a complex, originally called HflA, now called HflKC. HflKC interacts with FtsH; complex formation is stimulated by ATP, and with YccA.
3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261253, 452 interactors |
ComplexPortali | CPX-5046, FtsH-HflKC complex |
DIPi | DIP-47481N |
IntActi | P0ABC7, 12 interactors |
MINTi | P0ABC7 |
STRINGi | 511145.b4174 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0330, Bacteria |
HOGENOMi | CLU_039173_1_0_6 |
InParanoidi | P0ABC7 |
PhylomeDBi | P0ABC7 |
Family and domain databases
CDDi | cd03404, SPFH_HflK, 1 hit |
InterProi | View protein in InterPro IPR001107, Band_7 IPR036013, Band_7/SPFH_dom_sf IPR010201, HflK IPR020980, Membrane_HflK_N IPR001972, Stomatin_HflK_HflKC_fam |
PANTHERi | PTHR43327:SF2, PTHR43327:SF2, 1 hit |
Pfami | View protein in Pfam PF01145, Band_7, 1 hit PF12221, HflK_N, 1 hit |
PRINTSi | PR00721, STOMATIN |
SMARTi | View protein in SMART SM00244, PHB, 1 hit |
SUPFAMi | SSF117892, SSF117892, 1 hit |
TIGRFAMsi | TIGR01933, hflK, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABC7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAWNQPGNNG QDRDPWGSSK PGGNSEGNGN KGGRDQGPPD LDDIFRKLSK
60 70 80 90 100
KLGGLGGGKG TGSGGGSSSQ GPRPQLGGRV VTIAAAAIVI IWAASGFYTI
110 120 130 140 150
KEAERGVVTR FGKFSHLVEP GLNWKPTFID EVKPVNVEAV RELAASGVML
160 170 180 190 200
TSDENVVRVE MNVQYRVTNP EKYLYSVTSP DDSLRQATDS ALRGVIGKYT
210 220 230 240 250
MDRILTEGRT VIRSDTQREL EETIRPYDMG ITLLDVNFQA ARPPEEVKAA
260 270 280 290 300
FDDAIAAREN EQQYIREAEA YTNEVQPRAN GQAQRILEEA RAYKAQTILE
310 320 330 340 350
AQGEVARFAK LLPEYKAAPE ITRERLYIET MEKVLGNTRK VLVNDKGGNL
360 370 380 390 400
MVLPLDQMLK GGNAPAAKSD NGASNLLRLP PASSSTTSGA SNTSSTSQGD
410
IMDQRRANAQ RNDYQRQGE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00005 Unassigned DNA Translation: AAC43399.1 U14003 Genomic DNA Translation: AAA97070.1 U00096 Genomic DNA Translation: AAC77131.1 AP009048 Genomic DNA Translation: BAE78175.1 |
PIRi | B43653 |
RefSeqi | NP_418595.1, NC_000913.3 WP_000312488.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77131; AAC77131; b4174 BAE78175; BAE78175; BAE78175 |
GeneIDi | 58460291 948698 |
KEGGi | ecj:JW4132 eco:b4174 |
PATRICi | fig|1411691.4.peg.2527 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00005 Unassigned DNA Translation: AAC43399.1 U14003 Genomic DNA Translation: AAA97070.1 U00096 Genomic DNA Translation: AAC77131.1 AP009048 Genomic DNA Translation: BAE78175.1 |
PIRi | B43653 |
RefSeqi | NP_418595.1, NC_000913.3 WP_000312488.1, NZ_STEB01000013.1 |
3D structure databases
SMRi | P0ABC7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261253, 452 interactors |
ComplexPortali | CPX-5046, FtsH-HflKC complex |
DIPi | DIP-47481N |
IntActi | P0ABC7, 12 interactors |
MINTi | P0ABC7 |
STRINGi | 511145.b4174 |
Protein family/group databases
MEROPSi | I87.002 |
Proteomic databases
jPOSTi | P0ABC7 |
PaxDbi | P0ABC7 |
PRIDEi | P0ABC7 |
Genome annotation databases
EnsemblBacteriai | AAC77131; AAC77131; b4174 BAE78175; BAE78175; BAE78175 |
GeneIDi | 58460291 948698 |
KEGGi | ecj:JW4132 eco:b4174 |
PATRICi | fig|1411691.4.peg.2527 |
Organism-specific databases
EchoBASEi | EB0431 |
Phylogenomic databases
eggNOGi | COG0330, Bacteria |
HOGENOMi | CLU_039173_1_0_6 |
InParanoidi | P0ABC7 |
PhylomeDBi | P0ABC7 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10436-MONOMER MetaCyc:EG10436-MONOMER |
Miscellaneous databases
PROi | PR:P0ABC7 |
Family and domain databases
CDDi | cd03404, SPFH_HflK, 1 hit |
InterProi | View protein in InterPro IPR001107, Band_7 IPR036013, Band_7/SPFH_dom_sf IPR010201, HflK IPR020980, Membrane_HflK_N IPR001972, Stomatin_HflK_HflKC_fam |
PANTHERi | PTHR43327:SF2, PTHR43327:SF2, 1 hit |
Pfami | View protein in Pfam PF01145, Band_7, 1 hit PF12221, HflK_N, 1 hit |
PRINTSi | PR00721, STOMATIN |
SMARTi | View protein in SMART SM00244, PHB, 1 hit |
SUPFAMi | SSF117892, SSF117892, 1 hit |
TIGRFAMsi | TIGR01933, hflK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HFLK_ECOLI | |
Accessioni | P0ABC7Primary (citable) accession number: P0ABC7 Secondary accession number(s): P25662, Q2M6D1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 10, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families