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UniProtKB - P0ABC7 (HFLK_ECOLI)

Entry version 114 (29 Sep 2021)
Sequence version 1 (08 Nov 2005)
Previous versions | rss
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Protein

Modulator of FtsH protease HflK

Gene

hflK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

HflC and HflK help govern the stability of phage lambda cII protein, and thereby control the lysogenization frequency of phage lambda. HflKC inhibits the SecY-degrading activity of FtsH, possibly helping quality control of integral membrane proteins.

1 Publication

Miscellaneous

Integration of this protein into the membrane depends on SecA, SecY and SecD but not on SecB or FtsY. HflK is unstable in the absence of HflC.

Caution

Was originally (PubMed:2973057) thought to be a protease. However, removal of residues '165-200' of complex member HflC (a ClpP-protease-like motif) does not alter the lysogenization process, and in vitro studies show no evidence of a protease activity for the isolated HflKC complex.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

  • negative regulation of catalytic activity Source: EcoCyc
  • response to heat Source: EcoliWiki
Complete GO annotation on QuickGO ...

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG10436-MONOMER
MetaCyc:EG10436-MONOMER

Protein family/group databases

MEROPS protease database

More...MEROPSi		I87.002

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Modulator of FtsH protease HflK
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hflK
Synonyms:hflA
Ordered Locus Names:b4174, JW4132
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 79Cytoplasmic1 PublicationAdd BLAST79
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei80 – 100Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST21
Topological domaini101 – 419Periplasmic1 PublicationAdd BLAST319

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi145A → V in hflK13; stabilizes overproduced SecY but not overproduced cII protein. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000940851 – 419Modulator of FtsH protease HflKAdd BLAST419

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0ABC7

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0ABC7

PRoteomics IDEntifications database

More...PRIDEi		P0ABC7

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

HflC and HflK interact to form a complex, originally called HflA, now called HflKC. HflKC interacts with FtsH; complex formation is stimulated by ATP, and with YccA.

3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4261253, 452 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-5046, FtsH-HflKC complex

Database of interacting proteins

More...DIPi		DIP-47481N

Protein interaction database and analysis system

More...IntActi		P0ABC7, 12 interactors

Molecular INTeraction database

More...MINTi		P0ABC7

STRING: functional protein association networks

More...STRINGi		511145.b4174

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0ABC7

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 75DisorderedSequence analysisAdd BLAST75
Regioni364 – 419DisorderedSequence analysisAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1 – 15Polar residuesSequence analysisAdd BLAST15
Compositional biasi376 – 405Polar residuesSequence analysisAdd BLAST30

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the band 7/mec-2 family. HflK subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0330, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_039173_1_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0ABC7

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0ABC7

Family and domain databases

Conserved Domains Database

More...CDDi		cd03404, SPFH_HflK, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.479.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001107, Band_7
IPR036013, Band_7/SPFH_dom_sf
IPR010201, HflK
IPR020980, Membrane_HflK_N
IPR001972, Stomatin_HflK_HflKC_fam

The PANTHER Classification System

More...PANTHERi		PTHR43327:SF2, PTHR43327:SF2, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01145, Band_7, 1 hit
PF12221, HflK_N, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00721, STOMATIN

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00244, PHB, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF117892, SSF117892, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01933, hflK, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0ABC7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAWNQPGNNG QDRDPWGSSK PGGNSEGNGN KGGRDQGPPD LDDIFRKLSK 
        60         70         80         90        100
KLGGLGGGKG TGSGGGSSSQ GPRPQLGGRV VTIAAAAIVI IWAASGFYTI 
       110        120        130        140        150
KEAERGVVTR FGKFSHLVEP GLNWKPTFID EVKPVNVEAV RELAASGVML 
       160        170        180        190        200
TSDENVVRVE MNVQYRVTNP EKYLYSVTSP DDSLRQATDS ALRGVIGKYT 
       210        220        230        240        250
MDRILTEGRT VIRSDTQREL EETIRPYDMG ITLLDVNFQA ARPPEEVKAA 
       260        270        280        290        300
FDDAIAAREN EQQYIREAEA YTNEVQPRAN GQAQRILEEA RAYKAQTILE 
       310        320        330        340        350
AQGEVARFAK LLPEYKAAPE ITRERLYIET MEKVLGNTRK VLVNDKGGNL 
       360        370        380        390        400
MVLPLDQMLK GGNAPAAKSD NGASNLLRLP PASSSTTSGA SNTSSTSQGD 
       410 
IMDQRRANAQ RNDYQRQGE
Length:419
Mass (Da):45,545
Last modified:November 8, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE7B501DC70A49FBC
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U00005 Unassigned DNA Translation: AAC43399.1
U14003 Genomic DNA Translation: AAA97070.1
U00096 Genomic DNA Translation: AAC77131.1
AP009048 Genomic DNA Translation: BAE78175.1

Protein sequence database of the Protein Information Resource

More...PIRi		B43653

NCBI Reference Sequences

More...RefSeqi		NP_418595.1, NC_000913.3
WP_000312488.1, NZ_STEB01000013.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC77131; AAC77131; b4174
BAE78175; BAE78175; BAE78175

Database of genes from NCBI RefSeq genomes

More...GeneIDi		60666822
948698

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW4132
eco:b4174

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2527

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00005 Unassigned DNA Translation: AAC43399.1
U14003 Genomic DNA Translation: AAA97070.1
U00096 Genomic DNA Translation: AAC77131.1
AP009048 Genomic DNA Translation: BAE78175.1
PIRiB43653
RefSeqiNP_418595.1, NC_000913.3
WP_000312488.1, NZ_STEB01000013.1

3D structure databases

SMRiP0ABC7
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4261253, 452 interactors
ComplexPortaliCPX-5046, FtsH-HflKC complex
DIPiDIP-47481N
IntActiP0ABC7, 12 interactors
MINTiP0ABC7
STRINGi511145.b4174

Protein family/group databases

MEROPSiI87.002

Proteomic databases

jPOSTiP0ABC7
PaxDbiP0ABC7
PRIDEiP0ABC7

Genome annotation databases

EnsemblBacteriaiAAC77131; AAC77131; b4174
BAE78175; BAE78175; BAE78175
GeneIDi60666822
948698
KEGGiecj:JW4132
eco:b4174
PATRICifig|1411691.4.peg.2527

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0431

Phylogenomic databases

eggNOGiCOG0330, Bacteria
HOGENOMiCLU_039173_1_0_6
InParanoidiP0ABC7
PhylomeDBiP0ABC7

Enzyme and pathway databases

BioCyciEcoCyc:EG10436-MONOMER
MetaCyc:EG10436-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P0ABC7

Family and domain databases

CDDicd03404, SPFH_HflK, 1 hit
Gene3Di3.30.479.30, 1 hit
InterProiView protein in InterPro
IPR001107, Band_7
IPR036013, Band_7/SPFH_dom_sf
IPR010201, HflK
IPR020980, Membrane_HflK_N
IPR001972, Stomatin_HflK_HflKC_fam
PANTHERiPTHR43327:SF2, PTHR43327:SF2, 1 hit
PfamiView protein in Pfam
PF01145, Band_7, 1 hit
PF12221, HflK_N, 1 hit
PRINTSiPR00721, STOMATIN
SMARTiView protein in SMART
SM00244, PHB, 1 hit
SUPFAMiSSF117892, SSF117892, 1 hit
TIGRFAMsiTIGR01933, hflK, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHFLK_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0ABC7
Secondary accession number(s): P25662, Q2M6D1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 29, 2021
This is version 114 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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