UniProtKB - P0ABB0 (ATPA_ECOLI)
Protein
ATP synthase subunit alpha
Gene
atpA
Organism
Escherichia coli (strain K12)
Status
Functioni
Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.
Catalytic activityi
- EC:7.1.2.2UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 373 | Required for activity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 169 – 176 | ATPCurated | 8 |
GO - Molecular functioni
- ADP binding Source: GO_Central
- ATP binding Source: GO_Central
- proton-transporting ATPase activity, rotational mechanism Source: UniProtKB-EC
- proton-transporting ATP synthase activity, rotational mechanism Source: UniProtKB-UniRule
GO - Biological processi
- ATP synthesis coupled proton transport Source: EcoliWiki
Keywordsi
Molecular function | Translocase |
Biological process | ATP synthesis, Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ATPA-MONOMER MetaCyc:ATPA-MONOMER |
Protein family/group databases
TCDBi | 3.A.2.1.1, the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ATP synthase subunit alphaUniRule annotation (EC:7.1.2.2UniRule annotation)Alternative name(s): ATP synthase F1 sector subunit alphaUniRule annotation F-ATPase subunit alphaUniRule annotation |
Gene namesi | Name:atpAUniRule annotation Synonyms:papA, uncA Ordered Locus Names:b3734, JW3712 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation1 Publication
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- membrane Source: UniProtKB
- proton-transporting ATP synthase complex, catalytic core F(1) Source: EcoliWiki
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, CF(1), MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 175 | K → E: Reduced activity and reduced ATP-binding. 1 Publication | 1 | |
Mutagenesisi | 175 | K → I: Reduced activity and loss of ATP-binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144325 | 1 – 513 | ATP synthase subunit alphaAdd BLAST | 513 |
Proteomic databases
jPOSTi | P0ABB0 |
PaxDbi | P0ABB0 |
PRIDEi | P0ABB0 |
2D gel databases
SWISS-2DPAGEi | P0ABB0 |
Interactioni
Subunit structurei
F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
UniRule annotationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 852544, 9 interactors |
ComplexPortali | CPX-4022, ATP synthase complex |
DIPi | DIP-31845N |
IntActi | P0ABB0, 29 interactors |
MINTi | P0ABB0 |
STRINGi | 511145.b3734 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABB0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABB0 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATPase alpha/beta chains family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0056, Bacteria |
HOGENOMi | CLU_010091_2_1_6 |
InParanoidi | P0ABB0 |
PhylomeDBi | P0ABB0 |
Family and domain databases
CDDi | cd18113, ATP-synt_F1_alpha_C, 1 hit cd01132, F1_ATPase_alpha, 1 hit |
Gene3Di | 1.20.150.20, 1 hit 2.40.30.20, 1 hit |
HAMAPi | MF_01346, ATP_synth_alpha_bact, 1 hit |
InterProi | View protein in InterPro IPR023366, ATP_synth_asu-like_sf IPR000793, ATP_synth_asu_C IPR038376, ATP_synth_asu_C_sf IPR033732, ATP_synth_F1_a IPR005294, ATP_synth_F1_asu IPR020003, ATPase_a/bsu_AS IPR004100, ATPase_F1/V1/A1_a/bsu_N IPR036121, ATPase_F1/V1/A1_a/bsu_N_sf IPR000194, ATPase_F1/V1/A1_a/bsu_nucl-bd IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00006, ATP-synt_ab, 1 hit PF00306, ATP-synt_ab_C, 1 hit PF02874, ATP-synt_ab_N, 1 hit |
SUPFAMi | SSF50615, SSF50615, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00962, atpA, 1 hit |
PROSITEi | View protein in PROSITE PS00152, ATPASE_ALPHA_BETA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABB0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLNSTEISE LIKQRIAQFN VVSEAHNEGT IVSVSDGVIR IHGLADCMQG
60 70 80 90 100
EMISLPGNRY AIALNLERDS VGAVVMGPYA DLAEGMKVKC TGRILEVPVG
110 120 130 140 150
RGLLGRVVNT LGAPIDGKGP LDHDGFSAVE AIAPGVIERQ SVDQPVQTGY
160 170 180 190 200
KAVDSMIPIG RGQRELIIGD RQTGKTALAI DAIINQRDSG IKCIYVAIGQ
210 220 230 240 250
KASTISNVVR KLEEHGALAN TIVVVATASE SAALQYLAPY AGCAMGEYFR
260 270 280 290 300
DRGEDALIIY DDLSKQAVAY RQISLLLRRP PGREAFPGDV FYLHSRLLER
310 320 330 340 350
AARVNAEYVE AFTKGEVKGK TGSLTALPII ETQAGDVSAF VPTNVISITD
360 370 380 390 400
GQIFLETNLF NAGIRPAVNP GISVSRVGGA AQTKIMKKLS GGIRTALAQY
410 420 430 440 450
RELAAFSQFA SDLDDATRKQ LDHGQKVTEL LKQKQYAPMS VAQQSLVLFA
460 470 480 490 500
AERGYLADVE LSKIGSFEAA LLAYVDRDHA PLMQEINQTG GYNDEIEGKL
510
KGILDSFKAT QSW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 91 | T → I in AAA83873 (PubMed:6301339).Curated | 1 | |
Sequence conflicti | 239 – 244 | PYAGCA → RMPVAL in CAA25780 (PubMed:6301339).Curated | 6 | |
Sequence conflicti | 239 – 244 | PYAGCA → RMPVAL in AAA24735 (PubMed:6301339).Curated | 6 | |
Sequence conflicti | 300 – 302 | RAA → MLQ in CAA23519 (PubMed:6272228).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01631 Genomic DNA Translation: CAA25780.1 Sequence problems. J01594 Genomic DNA Translation: AAA24735.1 Sequence problems. V00265 Genomic DNA Translation: CAA23519.1 Sequence problems. V00312 Genomic DNA Translation: CAA23596.1 Sequence problems. M12212 Unassigned DNA Translation: AAA20045.1 M25464 Genomic DNA Translation: AAA83873.1 L10328 Genomic DNA Translation: AAA62086.1 U00096 Genomic DNA Translation: AAC76757.1 AP009048 Genomic DNA Translation: BAE77554.1 V00266 Genomic DNA Translation: CAA23525.1 |
PIRi | G65176, PWECA |
RefSeqi | NP_418190.1, NC_000913.3 WP_001176745.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76757; AAC76757; b3734 BAE77554; BAE77554; BAE77554 |
GeneIDi | 58461067 948242 |
KEGGi | ecj:JW3712 eco:b3734 |
PATRICi | fig|1411691.4.peg.2966 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01631 Genomic DNA Translation: CAA25780.1 Sequence problems. J01594 Genomic DNA Translation: AAA24735.1 Sequence problems. V00265 Genomic DNA Translation: CAA23519.1 Sequence problems. V00312 Genomic DNA Translation: CAA23596.1 Sequence problems. M12212 Unassigned DNA Translation: AAA20045.1 M25464 Genomic DNA Translation: AAA83873.1 L10328 Genomic DNA Translation: AAA62086.1 U00096 Genomic DNA Translation: AAC76757.1 AP009048 Genomic DNA Translation: BAE77554.1 V00266 Genomic DNA Translation: CAA23525.1 |
PIRi | G65176, PWECA |
RefSeqi | NP_418190.1, NC_000913.3 WP_001176745.1, NZ_STEB01000015.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D8S | X-ray | 4.40 | A/B/C | 1-513 | [»] | |
3OAA | X-ray | 3.26 | A/B/C/I/J/K/Q/R/S/Y/Z/a | 1-513 | [»] | |
5T4O | electron microscopy | 6.90 | A/B/C | 1-513 | [»] | |
5T4P | electron microscopy | 7.77 | A/B/C | 1-513 | [»] | |
5T4Q | electron microscopy | 8.53 | A/B/C | 1-513 | [»] | |
6OQR | electron microscopy | 3.10 | A/B/C | 1-513 | [»] | |
6OQS | electron microscopy | 3.30 | A/B/C | 1-513 | [»] | |
6OQT | electron microscopy | 3.10 | A/B/C | 1-513 | [»] | |
6OQU | electron microscopy | 3.20 | A/B/C | 1-513 | [»] | |
6OQV | electron microscopy | 3.30 | A/B/C | 1-513 | [»] | |
6OQW | electron microscopy | 3.10 | A/B/C | 1-513 | [»] | |
6PQV | electron microscopy | 3.30 | A/B/C | 1-513 | [»] | |
6WNQ | electron microscopy | 3.40 | A/B/C | 1-513 | [»] | |
6WNR | electron microscopy | 3.30 | A/B/C | 1-513 | [»] | |
SMRi | P0ABB0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 852544, 9 interactors |
ComplexPortali | CPX-4022, ATP synthase complex |
DIPi | DIP-31845N |
IntActi | P0ABB0, 29 interactors |
MINTi | P0ABB0 |
STRINGi | 511145.b3734 |
Protein family/group databases
TCDBi | 3.A.2.1.1, the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily |
2D gel databases
SWISS-2DPAGEi | P0ABB0 |
Proteomic databases
jPOSTi | P0ABB0 |
PaxDbi | P0ABB0 |
PRIDEi | P0ABB0 |
Genome annotation databases
EnsemblBacteriai | AAC76757; AAC76757; b3734 BAE77554; BAE77554; BAE77554 |
GeneIDi | 58461067 948242 |
KEGGi | ecj:JW3712 eco:b3734 |
PATRICi | fig|1411691.4.peg.2966 |
Organism-specific databases
EchoBASEi | EB0096 |
Phylogenomic databases
eggNOGi | COG0056, Bacteria |
HOGENOMi | CLU_010091_2_1_6 |
InParanoidi | P0ABB0 |
PhylomeDBi | P0ABB0 |
Enzyme and pathway databases
BioCyci | EcoCyc:ATPA-MONOMER MetaCyc:ATPA-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0ABB0 |
PROi | PR:P0ABB0 |
Family and domain databases
CDDi | cd18113, ATP-synt_F1_alpha_C, 1 hit cd01132, F1_ATPase_alpha, 1 hit |
Gene3Di | 1.20.150.20, 1 hit 2.40.30.20, 1 hit |
HAMAPi | MF_01346, ATP_synth_alpha_bact, 1 hit |
InterProi | View protein in InterPro IPR023366, ATP_synth_asu-like_sf IPR000793, ATP_synth_asu_C IPR038376, ATP_synth_asu_C_sf IPR033732, ATP_synth_F1_a IPR005294, ATP_synth_F1_asu IPR020003, ATPase_a/bsu_AS IPR004100, ATPase_F1/V1/A1_a/bsu_N IPR036121, ATPase_F1/V1/A1_a/bsu_N_sf IPR000194, ATPase_F1/V1/A1_a/bsu_nucl-bd IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00006, ATP-synt_ab, 1 hit PF00306, ATP-synt_ab_C, 1 hit PF02874, ATP-synt_ab_N, 1 hit |
SUPFAMi | SSF50615, SSF50615, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00962, atpA, 1 hit |
PROSITEi | View protein in PROSITE PS00152, ATPASE_ALPHA_BETA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATPA_ECOLI | |
Accessioni | P0ABB0Primary (citable) accession number: P0ABB0 Secondary accession number(s): P00822, Q2M852, Q47249 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families