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Protein

ATP synthase subunit b

Gene

atpF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation.UniRule annotation
Component of the F0 channel, it forms part of the peripheral stalk, linking F1 to F0.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei9Mutation prevents the formation of a functional proton pore, but has a small effect on the binding of F(1) to F(0)1
Sitei131Required for proton pore formation, as well as F(1) to F(0) binding1

GO - Molecular functioni

  • proton-transporting ATPase activity, rotational mechanism Source: EcoCyc
  • proton-transporting ATP synthase activity, rotational mechanism Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processATP synthesis, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:ATPF-MONOMER
MetaCyc:ATPF-MONOMER

Protein family/group databases

TCDBi3.A.2.1.1 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit bUniRule annotation
Alternative name(s):
ATP synthase F(0) sector subunit bUniRule annotation
ATPase subunit IUniRule annotation
F-type ATPase subunit bUniRule annotation
Short name:
F-ATPase subunit bUniRule annotation
Gene namesi
Name:atpFUniRule annotation
Synonyms:papF, uncF
Ordered Locus Names:b3736, JW3714
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10103 atpF

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • anchored component of membrane Source: EcoliWiki
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
  • proton-transporting ATP synthase complex, coupling factor F(o) Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, CF(0), Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB03091 4-Amido-4-Carbamoyl-Butyric Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000823711 – 156ATP synthase subunit bAdd BLAST156

Proteomic databases

EPDiP0ABA0
PaxDbiP0ABA0
PRIDEiP0ABA0

2D gel databases

SWISS-2DPAGEiP0ABA0

Interactioni

Subunit structurei

F-type ATPases have 2 components, F1 - the catalytic core - and F0 - the membrane proton channel. F1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. F0 has three main subunits: a1, b2 and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F1 is attached to F0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.UniRule annotation

Protein-protein interaction databases

BioGridi4262109, 80 interactors
DIPiDIP-35994N
IntActiP0ABA0, 2 interactors
STRINGi316385.ECDH10B_3923

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 24Combined sources21
Helixi26 – 33Combined sources8
Helixi63 – 120Combined sources58

3D structure databases

ProteinModelPortaliP0ABA0
SMRiP0ABA0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABA0

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase B chain family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107Z6K Bacteria
COG0711 LUCA
HOGENOMiHOG000015378
InParanoidiP0ABA0
KOiK02109
OMAiKFAWKPI
PhylomeDBiP0ABA0

Family and domain databases

HAMAPiMF_01398 ATP_synth_b_bprime, 1 hit
InterProiView protein in InterPro
IPR028987 ATP_synth_B-like_membr_sf
IPR002146 ATP_synth_b/b'su_bac/chlpt
IPR005864 ATP_synth_F0_bsu_bac
PfamiView protein in Pfam
PF00430 ATP-synt_B, 1 hit
SUPFAMiSSF81573 SSF81573, 1 hit
TIGRFAMsiTIGR01144 ATP_synt_b, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLNATILGQ AIAFVLFVLF CMKYVWPPLM AAIEKRQKEI ADGLASAERA
60 70 80 90 100
HKDLDLAKAS ATDQLKKAKA EAQVIIEQAN KRRSQILDEA KAEAEQERTK
110 120 130 140 150
IVAQAQAEIE AERKRAREEL RKQVAILAVA GAEKIIERSV DEAANSDIVD

KLVAEL
Length:156
Mass (Da):17,264
Last modified:July 21, 1986 - v1
Checksum:i51A93C8BEE9AD9DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33I → F in CAA23592 (PubMed:6277311).Curated1
Sequence conflicti62T → N in AAA24741 (PubMed:7686882).Curated1
Sequence conflicti72A → D in CAA23592 (PubMed:6277311).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01594 Genomic DNA Translation: AAA24733.1
X01631 Genomic DNA Translation: CAA25778.1
V00264 Genomic DNA Translation: CAA23516.1
V00310 Genomic DNA Translation: CAA23592.1
V00266 Genomic DNA Translation: CAA23523.1
M25464 Genomic DNA Translation: AAA83871.1
M10422 Genomic DNA Translation: AAA24741.1
L10328 Genomic DNA Translation: AAA62088.1
U00096 Genomic DNA Translation: AAC76759.1
AP009048 Genomic DNA Translation: BAE77552.1
M12212 Unassigned DNA Translation: AAA20043.1
PIRiD93732 LWECB
RefSeqiNP_418192.1, NC_000913.3
WP_001052219.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76759; AAC76759; b3736
BAE77552; BAE77552; BAE77552
GeneIDi948247
KEGGiecj:JW3714
eco:b3736
PATRICifig|1411691.4.peg.2964

Similar proteinsi

Entry informationi

Entry nameiATPF_ECOLI
AccessioniPrimary (citable) accession number: P0ABA0
Secondary accession number(s): P00859, Q2M854
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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