UniProtKB - P0ABA0 (ATPF_ECOLI)
Protein
ATP synthase subunit b
Gene
atpF
Organism
Escherichia coli (strain K12)
Status
Functioni
F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation.UniRule annotation
Component of the F0 channel, it forms part of the peripheral stalk, linking F1 to F0.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 9 | Mutation prevents the formation of a functional proton pore, but has a small effect on the binding of F(1) to F(0) | 1 | |
Sitei | 131 | Required for proton pore formation, as well as F(1) to F(0) binding | 1 |
GO - Molecular functioni
- proton-transporting ATPase activity, rotational mechanism Source: EcoCyc
- proton-transporting ATP synthase activity, rotational mechanism Source: EcoCyc
Keywordsi
Biological process | ATP synthesis, Hydrogen ion transport, Ion transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:ATPF-MONOMER MetaCyc:ATPF-MONOMER |
Protein family/group databases
TCDBi | 3.A.2.1.1, the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ATP synthase subunit bUniRule annotationAlternative name(s): ATP synthase F(0) sector subunit bUniRule annotation ATPase subunit IUniRule annotation F-type ATPase subunit bUniRule annotation Short name: F-ATPase subunit bUniRule annotation |
Gene namesi | Name:atpFUniRule annotation Synonyms:papF, uncF Ordered Locus Names:b3736, JW3714 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 11 – 31 | HelicalUniRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
- proton-transporting ATP synthase complex, coupling factor F(o) Source: EcoliWiki
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, CF(0), MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082371 | 1 – 156 | ATP synthase subunit bAdd BLAST | 156 |
Proteomic databases
jPOSTi | P0ABA0 |
PaxDbi | P0ABA0 |
PRIDEi | P0ABA0 |
2D gel databases
SWISS-2DPAGEi | P0ABA0 |
Interactioni
Subunit structurei
F-type ATPases have 2 components, F1 - the catalytic core - and F0 - the membrane proton channel. F1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. F0 has three main subunits: a1, b2 and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F1 is attached to F0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4262109, 80 interactors |
ComplexPortali | CPX-4022, ATP synthase complex |
DIPi | DIP-35994N |
IntActi | P0ABA0, 2 interactors |
STRINGi | 511145.b3736 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABA0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABA0 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATPase B chain family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0711, Bacteria |
HOGENOMi | CLU_079215_4_5_6 |
InParanoidi | P0ABA0 |
PhylomeDBi | P0ABA0 |
Family and domain databases
HAMAPi | MF_01398, ATP_synth_b_bprime, 1 hit |
InterProi | View protein in InterPro IPR028987, ATP_synth_B-like_membr_sf IPR002146, ATP_synth_b/b'su_bac/chlpt IPR005864, ATP_synth_F0_bsu_bac |
Pfami | View protein in Pfam PF00430, ATP-synt_B, 1 hit |
SUPFAMi | SSF81573, SSF81573, 1 hit |
TIGRFAMsi | TIGR01144, ATP_synt_b, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABA0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLNATILGQ AIAFVLFVLF CMKYVWPPLM AAIEKRQKEI ADGLASAERA
60 70 80 90 100
HKDLDLAKAS ATDQLKKAKA EAQVIIEQAN KRRSQILDEA KAEAEQERTK
110 120 130 140 150
IVAQAQAEIE AERKRAREEL RKQVAILAVA GAEKIIERSV DEAANSDIVD
KLVAEL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | I → F in CAA23592 (PubMed:6277311).Curated | 1 | |
Sequence conflicti | 62 | T → N in AAA24741 (PubMed:7686882).Curated | 1 | |
Sequence conflicti | 72 | A → D in CAA23592 (PubMed:6277311).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01594 Genomic DNA Translation: AAA24733.1 X01631 Genomic DNA Translation: CAA25778.1 V00264 Genomic DNA Translation: CAA23516.1 V00310 Genomic DNA Translation: CAA23592.1 V00266 Genomic DNA Translation: CAA23523.1 M25464 Genomic DNA Translation: AAA83871.1 M10422 Genomic DNA Translation: AAA24741.1 L10328 Genomic DNA Translation: AAA62088.1 U00096 Genomic DNA Translation: AAC76759.1 AP009048 Genomic DNA Translation: BAE77552.1 M12212 Unassigned DNA Translation: AAA20043.1 |
PIRi | D93732, LWECB |
RefSeqi | NP_418192.1, NC_000913.3 WP_001052219.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76759; AAC76759; b3736 BAE77552; BAE77552; BAE77552 |
GeneIDi | 58461069 948247 |
KEGGi | ecj:JW3714 eco:b3736 |
PATRICi | fig|1411691.4.peg.2964 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01594 Genomic DNA Translation: AAA24733.1 X01631 Genomic DNA Translation: CAA25778.1 V00264 Genomic DNA Translation: CAA23516.1 V00310 Genomic DNA Translation: CAA23592.1 V00266 Genomic DNA Translation: CAA23523.1 M25464 Genomic DNA Translation: AAA83871.1 M10422 Genomic DNA Translation: AAA24741.1 L10328 Genomic DNA Translation: AAA62088.1 U00096 Genomic DNA Translation: AAC76759.1 AP009048 Genomic DNA Translation: BAE77552.1 M12212 Unassigned DNA Translation: AAA20043.1 |
PIRi | D93732, LWECB |
RefSeqi | NP_418192.1, NC_000913.3 WP_001052219.1, NZ_STEB01000015.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B9U | NMR | - | A | 1-34 | [»] | |
1L2P | X-ray | 1.55 | A | 62-122 | [»] | |
2KHK | NMR | - | A | 30-82 | [»] | |
5T4O | electron microscopy | 6.90 | I/J | 2-156 | [»] | |
5T4P | electron microscopy | 7.77 | I/J | 2-156 | [»] | |
5T4Q | electron microscopy | 8.53 | I/J | 2-156 | [»] | |
6OQR | electron microscopy | 3.10 | X/Y | 1-156 | [»] | |
6OQS | electron microscopy | 3.30 | X/Y | 1-156 | [»] | |
6OQT | electron microscopy | 3.10 | X/Y | 1-156 | [»] | |
6OQU | electron microscopy | 3.20 | X/Y | 1-156 | [»] | |
6OQV | electron microscopy | 3.30 | X/Y | 1-156 | [»] | |
6OQW | electron microscopy | 3.10 | X/Y | 1-156 | [»] | |
6PQV | electron microscopy | 3.30 | X/Y | 1-156 | [»] | |
6VWK | electron microscopy | 3.30 | X/Y | 1-156 | [»] | |
6WNQ | electron microscopy | 3.40 | X/Y | 1-156 | [»] | |
6WNR | electron microscopy | 3.30 | X/Y | 1-156 | [»] | |
SMRi | P0ABA0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262109, 80 interactors |
ComplexPortali | CPX-4022, ATP synthase complex |
DIPi | DIP-35994N |
IntActi | P0ABA0, 2 interactors |
STRINGi | 511145.b3736 |
Chemistry databases
DrugBanki | DB03091, Isoglutamine |
Protein family/group databases
TCDBi | 3.A.2.1.1, the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily |
2D gel databases
SWISS-2DPAGEi | P0ABA0 |
Proteomic databases
jPOSTi | P0ABA0 |
PaxDbi | P0ABA0 |
PRIDEi | P0ABA0 |
Genome annotation databases
EnsemblBacteriai | AAC76759; AAC76759; b3736 BAE77552; BAE77552; BAE77552 |
GeneIDi | 58461069 948247 |
KEGGi | ecj:JW3714 eco:b3736 |
PATRICi | fig|1411691.4.peg.2964 |
Organism-specific databases
EchoBASEi | EB0101 |
Phylogenomic databases
eggNOGi | COG0711, Bacteria |
HOGENOMi | CLU_079215_4_5_6 |
InParanoidi | P0ABA0 |
PhylomeDBi | P0ABA0 |
Enzyme and pathway databases
BioCyci | EcoCyc:ATPF-MONOMER MetaCyc:ATPF-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0ABA0 |
PROi | PR:P0ABA0 |
Family and domain databases
HAMAPi | MF_01398, ATP_synth_b_bprime, 1 hit |
InterProi | View protein in InterPro IPR028987, ATP_synth_B-like_membr_sf IPR002146, ATP_synth_b/b'su_bac/chlpt IPR005864, ATP_synth_F0_bsu_bac |
Pfami | View protein in Pfam PF00430, ATP-synt_B, 1 hit |
SUPFAMi | SSF81573, SSF81573, 1 hit |
TIGRFAMsi | TIGR01144, ATP_synt_b, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATPF_ECOLI | |
Accessioni | P0ABA0Primary (citable) accession number: P0ABA0 Secondary accession number(s): P00859, Q2M854 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 10, 2021 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families