UniProtKB - P0AB91 (AROG_ECOLI)
Protein
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive
Gene
aroG
Organism
Escherichia coli (strain K12)
Status
Functioni
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Miscellaneous
There are 3 DAHP synthases, AroG is feedback-inhibited by Phe. The other 2 DAHP synthases are Tyr- and Trp-sensitive, respectively.
Catalytic activityi
- D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphateEC:2.5.1.54
: chorismate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.Proteins known to be involved in the 7 steps of the subpathway in this organism are:
- Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
- 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
- 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroD)
- Quinate/shikimate dehydrogenase (ydiB), Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
- Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK), Shikimate kinase 1 (aroK), Shikimate kinase 2 (aroL)
- 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
- Chorismate synthase (aroC), Chorismate synthase (aroC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.
GO - Molecular functioni
- 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: GO_Central
- chorismate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:AROG-MONOMER MetaCyc:AROG-MONOMER |
BRENDAi | 2.5.1.54, 2026 |
SABIO-RKi | P0AB91 |
UniPathwayi | UPA00053;UER00084 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (EC:2.5.1.54)Alternative name(s): 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase |
Gene namesi | Name:aroG Ordered Locus Names:b0754, JW0737 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
Pathology & Biotechi
Chemistry databases
DrugBanki | DB02726, 2-Phosphoglycolic Acid DB01819, Phosphoenolpyruvate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000140835 | 1 – 350 | Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitiveAdd BLAST | 350 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 244 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0AB91 |
PaxDbi | P0AB91 |
PRIDEi | P0AB91 |
2D gel databases
SWISS-2DPAGEi | P0AB91 |
PTM databases
iPTMneti | P0AB91 |
Interactioni
Subunit structurei
Homotetramer.
Binary interactionsi
P0AB91
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-1120055,EBI-1120055 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261709, 17 interactors |
DIPi | DIP-35898N |
IntActi | P0AB91, 3 interactors |
STRINGi | 511145.b0754 |
Chemistry databases
BindingDBi | P0AB91 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AB91 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AB91 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I DAHP synthase family.Curated
Phylogenomic databases
eggNOGi | COG0722, Bacteria |
HOGENOMi | CLU_030903_0_1_6 |
InParanoidi | P0AB91 |
PhylomeDBi | P0AB91 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006218, DAHP1/KDSA IPR006219, DHAP_synth_1 |
PANTHERi | PTHR21225, PTHR21225, 1 hit |
Pfami | View protein in Pfam PF00793, DAHP_synth_1, 1 hit |
PIRSFi | PIRSF001361, DAHP_synthase, 1 hit |
TIGRFAMsi | TIGR00034, aroFGH, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AB91-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNYQNDDLRI KEIKELLPPV ALLEKFPATE NAANTVAHAR KAIHKILKGN
60 70 80 90 100
DDRLLVVIGP CSIHDPVAAK EYATRLLALR EELKDELEIV MRVYFEKPRT
110 120 130 140 150
TVGWKGLIND PHMDNSFQIN DGLRIARKLL LDINDSGLPA AGEFLDMITP
160 170 180 190 200
QYLADLMSWG AIGARTTESQ VHRELASGLS CPVGFKNGTD GTIKVAIDAI
210 220 230 240 250
NAAGAPHCFL SVTKWGHSAI VNTSGNGDCH IILRGGKEPN YSAKHVAEVK
260 270 280 290 300
EGLNKAGLPA QVMIDFSHAN SSKQFKKQMD VCADVCQQIA GGEKAIIGVM
310 320 330 340 350
VESHLVEGNQ SLESGEPLAY GKSITDACIG WEDTDALLRQ LANAVKARRG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01591 Genomic DNA Translation: AAA23492.1 U00096 Genomic DNA Translation: AAC73841.1 AP009048 Genomic DNA Translation: BAA35416.1 |
PIRi | A01106, ADECHF |
RefSeqi | NP_415275.1, NC_000913.3 WP_001109196.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73841; AAC73841; b0754 BAA35416; BAA35416; BAA35416 |
GeneIDi | 945605 |
KEGGi | ecj:JW0737 eco:b0754 |
PATRICi | fig|1411691.4.peg.1525 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01591 Genomic DNA Translation: AAA23492.1 U00096 Genomic DNA Translation: AAC73841.1 AP009048 Genomic DNA Translation: BAA35416.1 |
PIRi | A01106, ADECHF |
RefSeqi | NP_415275.1, NC_000913.3 WP_001109196.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GG1 | X-ray | 2.00 | A/B/C/D | 1-350 | [»] | |
1KFL | X-ray | 2.80 | A/B/C/D/E/F/G/H | 2-350 | [»] | |
1N8F | X-ray | 1.75 | A/B/C/D | 1-350 | [»] | |
1QR7 | X-ray | 2.60 | A/B/C/D | 1-350 | [»] | |
5CKS | X-ray | 2.12 | A/B/C/D | 1-350 | [»] | |
SMRi | P0AB91 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261709, 17 interactors |
DIPi | DIP-35898N |
IntActi | P0AB91, 3 interactors |
STRINGi | 511145.b0754 |
Chemistry databases
BindingDBi | P0AB91 |
DrugBanki | DB02726, 2-Phosphoglycolic Acid DB01819, Phosphoenolpyruvate |
PTM databases
iPTMneti | P0AB91 |
2D gel databases
SWISS-2DPAGEi | P0AB91 |
Proteomic databases
jPOSTi | P0AB91 |
PaxDbi | P0AB91 |
PRIDEi | P0AB91 |
Genome annotation databases
EnsemblBacteriai | AAC73841; AAC73841; b0754 BAA35416; BAA35416; BAA35416 |
GeneIDi | 945605 |
KEGGi | ecj:JW0737 eco:b0754 |
PATRICi | fig|1411691.4.peg.1525 |
Organism-specific databases
EchoBASEi | EB0077 |
Phylogenomic databases
eggNOGi | COG0722, Bacteria |
HOGENOMi | CLU_030903_0_1_6 |
InParanoidi | P0AB91 |
PhylomeDBi | P0AB91 |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00084 |
BioCyci | EcoCyc:AROG-MONOMER MetaCyc:AROG-MONOMER |
BRENDAi | 2.5.1.54, 2026 |
SABIO-RKi | P0AB91 |
Miscellaneous databases
EvolutionaryTracei | P0AB91 |
PROi | PR:P0AB91 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006218, DAHP1/KDSA IPR006219, DHAP_synth_1 |
PANTHERi | PTHR21225, PTHR21225, 1 hit |
Pfami | View protein in Pfam PF00793, DAHP_synth_1, 1 hit |
PIRSFi | PIRSF001361, DAHP_synthase, 1 hit |
TIGRFAMsi | TIGR00034, aroFGH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROG_ECOLI | |
Accessioni | P0AB91Primary (citable) accession number: P0AB91 Secondary accession number(s): P00886 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 10, 2021 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families