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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive

Gene

aroG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Miscellaneous

There are 3 DAHP synthases, AroG is feedback-inhibited by Phe. The other 2 DAHP synthases are Tyr- and Trp-sensitive, respectively.

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Quinate/shikimate dehydrogenase (ydiB)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: GO_Central
  • chorismate biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:AROG-MONOMER
MetaCyc:AROG-MONOMER
BRENDAi2.5.1.54 2026
SABIO-RKiP0AB91
UniPathwayi
UPA00053;UER00084

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroG
Ordered Locus Names:b0754, JW0737
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10079 aroG

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB02726 2-Phosphoglycolic Acid
DB01819 Phosphoenolpyruvate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408351 – 350Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitiveAdd BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei244N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0AB91
PaxDbiP0AB91
PRIDEiP0AB91

2D gel databases

SWISS-2DPAGEiP0AB91

PTM databases

iPTMnetiP0AB91

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-1120055,EBI-1120055

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261709, 17 interactors
DIPiDIP-35898N
IntActiP0AB91, 3 interactors
STRINGi316385.ECDH10B_0821

Chemistry databases

BindingDBiP0AB91

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AB91
SMRiP0AB91
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AB91

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105E99 Bacteria
COG0722 LUCA
HOGENOMiHOG000220501
InParanoidiP0AB91
KOiK01626
PhylomeDBiP0AB91

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit

Sequencei

Sequence statusi: Complete.

P0AB91-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNYQNDDLRI KEIKELLPPV ALLEKFPATE NAANTVAHAR KAIHKILKGN
60 70 80 90 100
DDRLLVVIGP CSIHDPVAAK EYATRLLALR EELKDELEIV MRVYFEKPRT
110 120 130 140 150
TVGWKGLIND PHMDNSFQIN DGLRIARKLL LDINDSGLPA AGEFLDMITP
160 170 180 190 200
QYLADLMSWG AIGARTTESQ VHRELASGLS CPVGFKNGTD GTIKVAIDAI
210 220 230 240 250
NAAGAPHCFL SVTKWGHSAI VNTSGNGDCH IILRGGKEPN YSAKHVAEVK
260 270 280 290 300
EGLNKAGLPA QVMIDFSHAN SSKQFKKQMD VCADVCQQIA GGEKAIIGVM
310 320 330 340 350
VESHLVEGNQ SLESGEPLAY GKSITDACIG WEDTDALLRQ LANAVKARRG
Length:350
Mass (Da):38,010
Last modified:July 21, 1986 - v1
Checksum:i7477D361962E8710
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01591 Genomic DNA Translation: AAA23492.1
U00096 Genomic DNA Translation: AAC73841.1
AP009048 Genomic DNA Translation: BAA35416.1
PIRiA01106 ADECHF
RefSeqiNP_415275.1, NC_000913.3
WP_001109196.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73841; AAC73841; b0754
BAA35416; BAA35416; BAA35416
GeneIDi945605
KEGGiecj:JW0737
eco:b0754
PATRICifig|1411691.4.peg.1525

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01591 Genomic DNA Translation: AAA23492.1
U00096 Genomic DNA Translation: AAC73841.1
AP009048 Genomic DNA Translation: BAA35416.1
PIRiA01106 ADECHF
RefSeqiNP_415275.1, NC_000913.3
WP_001109196.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GG1X-ray2.00A/B/C/D1-350[»]
1KFLX-ray2.80A/B/C/D/E/F/G/H2-350[»]
1N8FX-ray1.75A/B/C/D1-350[»]
1QR7X-ray2.60A/B/C/D1-350[»]
5CKSX-ray2.12A/B/C/D1-350[»]
ProteinModelPortaliP0AB91
SMRiP0AB91
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261709, 17 interactors
DIPiDIP-35898N
IntActiP0AB91, 3 interactors
STRINGi316385.ECDH10B_0821

Chemistry databases

BindingDBiP0AB91
DrugBankiDB02726 2-Phosphoglycolic Acid
DB01819 Phosphoenolpyruvate

PTM databases

iPTMnetiP0AB91

2D gel databases

SWISS-2DPAGEiP0AB91

Proteomic databases

EPDiP0AB91
PaxDbiP0AB91
PRIDEiP0AB91

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73841; AAC73841; b0754
BAA35416; BAA35416; BAA35416
GeneIDi945605
KEGGiecj:JW0737
eco:b0754
PATRICifig|1411691.4.peg.1525

Organism-specific databases

EchoBASEiEB0077
EcoGeneiEG10079 aroG

Phylogenomic databases

eggNOGiENOG4105E99 Bacteria
COG0722 LUCA
HOGENOMiHOG000220501
InParanoidiP0AB91
KOiK01626
PhylomeDBiP0AB91

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00084

BioCyciEcoCyc:AROG-MONOMER
MetaCyc:AROG-MONOMER
BRENDAi2.5.1.54 2026
SABIO-RKiP0AB91

Miscellaneous databases

EvolutionaryTraceiP0AB91
PROiPR:P0AB91

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAROG_ECOLI
AccessioniPrimary (citable) accession number: P0AB91
Secondary accession number(s): P00886
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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