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Protein

Endonuclease III

Gene

nth

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation3 Publications

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotation2 PublicationsNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in DNA-binding and proper positioning of the enzyme along the DNA strand.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi187Iron-sulfur (4Fe-4S)Combined sources1 Publication1
Metal bindingi194Iron-sulfur (4Fe-4S)Combined sources1 Publication1
Metal bindingi197Iron-sulfur (4Fe-4S)Combined sources1 Publication1
Metal bindingi203Iron-sulfur (4Fe-4S)Combined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10662-MONOMER
MetaCyc:EG10662-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:b1633, JW1625
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10662 nth

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120K → Q: 100000-fold decrease in activity and slight decrease in substrate affinity. 1 Publication1
Mutagenesisi138D → N: 100-fold decrease in activity and 4-fold increase in substrate affinity. 1 Publication1
Mutagenesisi191K → E: Slight decrease in activity and 130-fold increase in substrate affinity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022101 – 211Endonuclease IIIAdd BLAST211

Proteomic databases

PaxDbiP0AB83
PRIDEiP0AB83

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG83EBI-555213,EBI-542683

Protein-protein interaction databases

BioGridi4262186, 123 interactors
DIPiDIP-48071N
IntActiP0AB83, 6 interactors
STRINGi316385.ECDH10B_1767

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AB83
SMRiP0AB83
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AB83

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 127HhHUniRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSM Bacteria
COG0177 LUCA
HOGENOMiHOG000252206
InParanoidiP0AB83
KOiK10773
OMAiKAKNPLC
PhylomeDBiP0AB83

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR01083 nth, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0AB83-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA
60 70 80 90 100
TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH
110 120 130 140 150
NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF
160 170 180 190 200
APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE
210
DLCEYKEKVD I
Length:211
Mass (Da):23,562
Last modified:November 8, 2005 - v1
Checksum:iFF4245823B7F902B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02857 Genomic DNA Translation: AAA24227.1
U00096 Genomic DNA Translation: AAC74705.1
AP009048 Genomic DNA Translation: BAA15387.1
PIRiA32412
RefSeqiNP_416150.1, NC_000913.3
WP_001030339.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74705; AAC74705; b1633
BAA15387; BAA15387; BAA15387
GeneIDi947122
KEGGiecj:JW1625
eco:b1633
PATRICifig|1411691.4.peg.628

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02857 Genomic DNA Translation: AAA24227.1
U00096 Genomic DNA Translation: AAC74705.1
AP009048 Genomic DNA Translation: BAA15387.1
PIRiA32412
RefSeqiNP_416150.1, NC_000913.3
WP_001030339.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ABKX-ray1.85A1-211[»]
ProteinModelPortaliP0AB83
SMRiP0AB83
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262186, 123 interactors
DIPiDIP-48071N
IntActiP0AB83, 6 interactors
STRINGi316385.ECDH10B_1767

Proteomic databases

PaxDbiP0AB83
PRIDEiP0AB83

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74705; AAC74705; b1633
BAA15387; BAA15387; BAA15387
GeneIDi947122
KEGGiecj:JW1625
eco:b1633
PATRICifig|1411691.4.peg.628

Organism-specific databases

EchoBASEiEB0656
EcoGeneiEG10662 nth

Phylogenomic databases

eggNOGiENOG4105CSM Bacteria
COG0177 LUCA
HOGENOMiHOG000252206
InParanoidiP0AB83
KOiK10773
OMAiKAKNPLC
PhylomeDBiP0AB83

Enzyme and pathway databases

BioCyciEcoCyc:EG10662-MONOMER
MetaCyc:EG10662-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AB83
PROiPR:P0AB83

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR01083 nth, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEND3_ECOLI
AccessioniPrimary (citable) accession number: P0AB83
Secondary accession number(s): P20625
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: October 10, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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