UniProtKB - P0AB83 (END3_ECOLI)
Protein
Endonuclease III
Gene
nth
Organism
Escherichia coli (strain K12)
Status
Functioni
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation3 Publications
Catalytic activityi
- The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation1 Publication EC:4.2.99.18
Cofactori
[4Fe-4S] clusterUniRule annotation2 PublicationsNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in DNA-binding and proper positioning of the enzyme along the DNA strand.UniRule annotation2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 187 | Iron-sulfur (4Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 194 | Iron-sulfur (4Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 197 | Iron-sulfur (4Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 203 | Iron-sulfur (4Fe-4S)Combined sources1 Publication | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- class I DNA-(apurinic or apyrimidinic site) endonuclease activity Source: UniProtKB-EC
- DNA-(apurinic or apyrimidinic site) endonuclease activity Source: EcoCyc
- DNA binding Source: UniProtKB-UniRule
- DNA N-glycosylase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: EcoCyc
- uracil DNA N-glycosylase activity Source: EcoCyc
GO - Biological processi
- base-excision repair, AP site formation Source: EcoCyc
- base-excision repair, AP site formation via deaminated base removal Source: EcoCyc
- cellular response to UV Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Glycosidase, Hydrolase, Lyase |
Biological process | DNA damage, DNA repair |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10662-MONOMER MetaCyc:EG10662-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)Alternative name(s): DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation |
Gene namesi | Name:nthUniRule annotation Ordered Locus Names:b1633, JW1625 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 120 | K → Q: 100000-fold decrease in activity and slight decrease in substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 138 | D → N: 100-fold decrease in activity and 4-fold increase in substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 191 | K → E: Slight decrease in activity and 130-fold increase in substrate affinity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102210 | 1 – 211 | Endonuclease IIIAdd BLAST | 211 |
Proteomic databases
jPOSTi | P0AB83 |
PaxDbi | P0AB83 |
PRIDEi | P0AB83 |
Interactioni
Subunit structurei
Monomer.
1 PublicationBinary interactionsi
P0AB83
With | #Exp. | IntAct |
---|---|---|
aceE [P0AFG8] | 3 | EBI-555213,EBI-542683 |
Protein-protein interaction databases
BioGRIDi | 4262186, 123 interactors |
DIPi | DIP-48071N |
IntActi | P0AB83, 6 interactors |
STRINGi | 511145.b1633 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AB83 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AB83 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 127 | HhHUniRule annotationAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the Nth/MutY family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0177, Bacteria |
HOGENOMi | CLU_012862_3_0_6 |
InParanoidi | P0AB83 |
PhylomeDBi | P0AB83 |
Family and domain databases
CDDi | cd00056, ENDO3c, 1 hit |
Gene3Di | 1.10.1670.10, 1 hit |
HAMAPi | MF_00942, Nth, 1 hit |
InterProi | View protein in InterPro IPR011257, DNA_glycosylase IPR004036, Endonuclease-III-like_CS2 IPR003651, Endonuclease3_FeS-loop_motif IPR004035, Endouclease-III_FeS-bd_BS IPR003265, HhH-GPD_domain IPR000445, HhH_motif IPR023170, HTH_base_excis_C IPR005759, Nth |
Pfami | View protein in Pfam PF10576, EndIII_4Fe-2S, 1 hit PF00633, HHH, 1 hit PF00730, HhH-GPD, 1 hit |
SMARTi | View protein in SMART SM00478, ENDO3c, 1 hit SM00525, FES, 1 hit |
SUPFAMi | SSF48150, SSF48150, 1 hit |
TIGRFAMsi | TIGR01083, nth, 1 hit |
PROSITEi | View protein in PROSITE PS00764, ENDONUCLEASE_III_1, 1 hit PS01155, ENDONUCLEASE_III_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AB83-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA
60 70 80 90 100
TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH
110 120 130 140 150
NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF
160 170 180 190 200
APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE
210
DLCEYKEKVD I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02857 Genomic DNA Translation: AAA24227.1 U00096 Genomic DNA Translation: AAC74705.1 AP009048 Genomic DNA Translation: BAA15387.1 |
PIRi | A32412 |
RefSeqi | NP_416150.1, NC_000913.3 WP_001030339.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74705; AAC74705; b1633 BAA15387; BAA15387; BAA15387 |
GeneIDi | 57731028 947122 |
KEGGi | ecj:JW1625 eco:b1633 |
PATRICi | fig|1411691.4.peg.628 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02857 Genomic DNA Translation: AAA24227.1 U00096 Genomic DNA Translation: AAC74705.1 AP009048 Genomic DNA Translation: BAA15387.1 |
PIRi | A32412 |
RefSeqi | NP_416150.1, NC_000913.3 WP_001030339.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ABK | X-ray | 1.85 | A | 1-211 | [»] | |
SMRi | P0AB83 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262186, 123 interactors |
DIPi | DIP-48071N |
IntActi | P0AB83, 6 interactors |
STRINGi | 511145.b1633 |
Proteomic databases
jPOSTi | P0AB83 |
PaxDbi | P0AB83 |
PRIDEi | P0AB83 |
Genome annotation databases
EnsemblBacteriai | AAC74705; AAC74705; b1633 BAA15387; BAA15387; BAA15387 |
GeneIDi | 57731028 947122 |
KEGGi | ecj:JW1625 eco:b1633 |
PATRICi | fig|1411691.4.peg.628 |
Organism-specific databases
EchoBASEi | EB0656 |
Phylogenomic databases
eggNOGi | COG0177, Bacteria |
HOGENOMi | CLU_012862_3_0_6 |
InParanoidi | P0AB83 |
PhylomeDBi | P0AB83 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10662-MONOMER MetaCyc:EG10662-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AB83 |
PROi | PR:P0AB83 |
Family and domain databases
CDDi | cd00056, ENDO3c, 1 hit |
Gene3Di | 1.10.1670.10, 1 hit |
HAMAPi | MF_00942, Nth, 1 hit |
InterProi | View protein in InterPro IPR011257, DNA_glycosylase IPR004036, Endonuclease-III-like_CS2 IPR003651, Endonuclease3_FeS-loop_motif IPR004035, Endouclease-III_FeS-bd_BS IPR003265, HhH-GPD_domain IPR000445, HhH_motif IPR023170, HTH_base_excis_C IPR005759, Nth |
Pfami | View protein in Pfam PF10576, EndIII_4Fe-2S, 1 hit PF00633, HHH, 1 hit PF00730, HhH-GPD, 1 hit |
SMARTi | View protein in SMART SM00478, ENDO3c, 1 hit SM00525, FES, 1 hit |
SUPFAMi | SSF48150, SSF48150, 1 hit |
TIGRFAMsi | TIGR01083, nth, 1 hit |
PROSITEi | View protein in PROSITE PS00764, ENDONUCLEASE_III_1, 1 hit PS01155, ENDONUCLEASE_III_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | END3_ECOLI | |
Accessioni | P0AB83Primary (citable) accession number: P0AB83 Secondary accession number(s): P20625 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families