UniProtKB - P0AB67 (PNTB_ECOLI)
Protein
NAD(P) transhydrogenase subunit beta
Gene
pntB
Organism
Escherichia coli (strain K12)
Status
Functioni
The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Catalytic activityi
- EC:7.1.1.1
GO - Molecular functioni
- NAD(P)+ transhydrogenase (AB-specific) activity Source: EcoCyc
- NAD(P)+ transhydrogenase activity Source: EcoCyc
- NADP binding Source: EcoCyc
GO - Biological processi
- NADPH regeneration Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Ligand | NAD, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:PNTB-MONOMER MetaCyc:PNTB-MONOMER |
Protein family/group databases
TCDBi | 3.D.2.1.1, the proton-translocating transhydrogenase (pth) family |
Names & Taxonomyi
Protein namesi | Recommended name: NAD(P) transhydrogenase subunit beta (EC:7.1.1.1)Alternative name(s): Nicotinamide nucleotide transhydrogenase subunit beta Pyridine nucleotide transhydrogenase subunit beta |
Gene namesi | Name:pntB Ordered Locus Names:b1602, JW1594 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 3 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 4 – 24 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 25 – 45 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 46 – 66 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 67 – 82 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 83 – 103 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 104 – 115 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 116 – 136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 164 | PeriplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 165 – 185 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 186 – 188 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 210 – 215 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 216 – 236 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 237 – 239 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 240 – 260 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 261 – 308 | PeriplasmicSequence analysisAdd BLAST | 48 | |
Transmembranei | 309 – 329 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 330 – 462 | CytoplasmicSequence analysisAdd BLAST | 133 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199023 | 1 – 462 | NAD(P) transhydrogenase subunit betaAdd BLAST | 462 |
Proteomic databases
jPOSTi | P0AB67 |
PaxDbi | P0AB67 |
PRIDEi | P0AB67 |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta chain.
Protein-protein interaction databases
BioGRIDi | 4259125, 34 interactors |
ComplexPortali | CPX-5623, NAD(P) transhydrogenase complex |
DIPi | DIP-367N |
STRINGi | 511145.b1602 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AB67 |
SMRi | P0AB67 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AB67 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PNT beta subunit family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1282, Bacteria |
HOGENOMi | CLU_007866_4_0_6 |
InParanoidi | P0AB67 |
PhylomeDBi | P0AB67 |
Family and domain databases
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR012136, NADH_DH_b IPR034300, PNTB-like |
Pfami | View protein in Pfam PF02233, PNTB, 1 hit |
PIRSFi | PIRSF000204, PNTB, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AB67-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGGLVTAAY IVAAILFIFS LAGLSKHETS RQGNNFGIAG MAIALIATIF
60 70 80 90 100
GPDTGNVGWI LLAMVIGGAI GIRLAKKVEM TEMPELVAIL HSFVGLAAVL
110 120 130 140 150
VGFNSYLHHD AGMAPILVNI HLTEVFLGIF IGAVTFTGSV VAFGKLCGKI
160 170 180 190 200
SSKPLMLPNR HKMNLAALVV SFLLLIVFVR TDSVGLQVLA LLIMTAIALV
210 220 230 240 250
FGWHLVASIG GADMPVVVSM LNSYSGWAAA AAGFMLSNDL LIVTGALVGS
260 270 280 290 300
SGAILSYIMC KAMNRSFISV IAGGFGTDGS STGDDQEVGE HREITAEETA
310 320 330 340 350
ELLKNSHSVI ITPGYGMAVA QAQYPVAEIT EKLRARGINV RFGIHPVAGR
360 370 380 390 400
LPGHMNVLLA EAKVPYDIVL EMDEINDDFA DTDTVLVIGA NDTVNPAAQD
410 420 430 440 450
DPKSPIAGMP VLEVWKAQNV IVFKRSMNTG YAGVQNPLFF KENTHMLFGD
460
AKASVDAILK AL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04195 Genomic DNA Translation: CAB37090.1 X66086 Genomic DNA Translation: CAA46885.1 U00096 Genomic DNA Translation: AAC74674.1 AP009048 Genomic DNA Translation: BAA15336.1 |
PIRi | S24381, DEECXB |
RefSeqi | NP_416119.1, NC_000913.3 WP_000014036.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74674; AAC74674; b1602 BAA15336; BAA15336; BAA15336 |
GeneIDi | 58459787 946144 |
KEGGi | ecj:JW1594 eco:b1602 |
PATRICi | fig|1411691.4.peg.660 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04195 Genomic DNA Translation: CAB37090.1 X66086 Genomic DNA Translation: CAA46885.1 U00096 Genomic DNA Translation: AAC74674.1 AP009048 Genomic DNA Translation: BAA15336.1 |
PIRi | S24381, DEECXB |
RefSeqi | NP_416119.1, NC_000913.3 WP_000014036.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BRU | NMR | - | C | 286-462 | [»] | |
BMRBi | P0AB67 | |||||
SMRi | P0AB67 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259125, 34 interactors |
ComplexPortali | CPX-5623, NAD(P) transhydrogenase complex |
DIPi | DIP-367N |
STRINGi | 511145.b1602 |
Protein family/group databases
TCDBi | 3.D.2.1.1, the proton-translocating transhydrogenase (pth) family |
Proteomic databases
jPOSTi | P0AB67 |
PaxDbi | P0AB67 |
PRIDEi | P0AB67 |
Genome annotation databases
EnsemblBacteriai | AAC74674; AAC74674; b1602 BAA15336; BAA15336; BAA15336 |
GeneIDi | 58459787 946144 |
KEGGi | ecj:JW1594 eco:b1602 |
PATRICi | fig|1411691.4.peg.660 |
Organism-specific databases
EchoBASEi | EB0738 |
Phylogenomic databases
eggNOGi | COG1282, Bacteria |
HOGENOMi | CLU_007866_4_0_6 |
InParanoidi | P0AB67 |
PhylomeDBi | P0AB67 |
Enzyme and pathway databases
BioCyci | EcoCyc:PNTB-MONOMER MetaCyc:PNTB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AB67 |
PROi | PR:P0AB67 |
Family and domain databases
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR012136, NADH_DH_b IPR034300, PNTB-like |
Pfami | View protein in Pfam PF02233, PNTB, 1 hit |
PIRSFi | PIRSF000204, PNTB, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PNTB_ECOLI | |
Accessioni | P0AB67Primary (citable) accession number: P0AB67 Secondary accession number(s): P07002, P76890 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 10, 2021 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families