UniProtKB - P0AB65 (ACYP_ECOLI)
Protein
Acylphosphatase
Gene
yccX
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
Has a considerably reduced catalytic efficiency compared to other mesophilic acylphosphatases. Shows a considerable resistance against urea denaturation since the full enzymatic activity is maintained in the presence of urea concentrations approaching 6.0 M and that only a slight decreae of 10-15 % was observed with higher urea concentrations.1 Publication
Catalytic activityi
- EC:3.6.1.7UniRule annotation1 Publication
Kineticsi
- KM=0.54 mM for benzoylphosphate1 Publication
pH dependencei
Optimum pH is 5.2-6.5.1 Publication
Temperature dependencei
Thermostable. Retains 85-90 % of its activity after 3 hours of incubation at 90 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 20 | UniRule annotation | 1 | |
Active sitei | 38 | UniRule annotation | 1 |
GO - Molecular functioni
- acylphosphatase activity Source: EcoCyc
GO - Biological processi
- response to heat Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6502-MONOMER MetaCyc:G6502-MONOMER |
BRENDAi | 3.6.1.7, 2026 |
SABIO-RKi | P0AB65 |
Names & Taxonomyi
Protein namesi | Recommended name: Acylphosphatase2 PublicationsUniRule annotation (EC:3.6.1.7UniRule annotation1 Publication)Alternative name(s): Acylphosphate phosphohydrolaseUniRule annotationCurated |
Gene namesi | Name:yccXUniRule annotation Ordered Locus Names:b0968, JW5131 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | C → A: No change in secondary structure. Nearly identical enzymatic properties. Loss of activity in 8.0 M urea. Reduced thermodynamic activity. Inactivated after 3 hours of incubation at 90 degrees Celsius. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000158553 | 1 – 92 | AcylphosphataseAdd BLAST | 92 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 5 ↔ 49 | UniRule annotation1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P0AB65 |
PaxDbi | P0AB65 |
PRIDEi | P0AB65 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260041, 6 interactors 849681, 3 interactors |
DIPi | DIP-11502N |
IntActi | P0AB65, 4 interactors |
STRINGi | 511145.b0968 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AB65 |
SMRi | P0AB65 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AB65 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 92 | Acylphosphatase-likeUniRule annotationAdd BLAST | 88 |
Sequence similaritiesi
Belongs to the acylphosphatase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1254, Bacteria |
HOGENOMi | CLU_141932_1_2_6 |
InParanoidi | P0AB65 |
PhylomeDBi | P0AB65 |
Family and domain databases
HAMAPi | MF_01450, Acylphosphatase_entero, 1 hit |
InterProi | View protein in InterPro IPR020456, Acylphosphatase IPR001792, Acylphosphatase-like_dom IPR036046, Acylphosphatase-like_dom_sf IPR028627, Acylphosphatase_bac IPR017968, Acylphosphatase_CS |
PANTHERi | PTHR47268, PTHR47268, 1 hit |
Pfami | View protein in Pfam PF00708, Acylphosphatase, 1 hit |
PRINTSi | PR00112, ACYLPHPHTASE |
SUPFAMi | SSF54975, SSF54975, 1 hit |
PROSITEi | View protein in PROSITE PS00150, ACYLPHOSPHATASE_1, 1 hit PS00151, ACYLPHOSPHATASE_2, 1 hit PS51160, ACYLPHOSPHATASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AB65-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKVCIIAWV YGRVQGVGFR YTTQYEAKRL GLTGYAKNLD DGSVEVVACG
60 70 80 90
EEGQVEKLMQ WLKSGGPRSA RVERVLSEPH HPSGELTDFR IR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74054.1 AP009048 Genomic DNA Translation: BAA35733.2 |
PIRi | G64837 |
RefSeqi | NP_415488.1, NC_000913.3 WP_000048252.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC74054; AAC74054; b0968 BAA35733; BAA35733; BAA35733 |
GeneIDi | 58346666 945304 |
KEGGi | ecj:JW5131 eco:b0968 |
PATRICi | fig|1411691.4.peg.1305 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74054.1 AP009048 Genomic DNA Translation: BAA35733.2 |
PIRi | G64837 |
RefSeqi | NP_415488.1, NC_000913.3 WP_000048252.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GV1 | NMR | - | A | 1-92 | [»] | |
BMRBi | P0AB65 | |||||
SMRi | P0AB65 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260041, 6 interactors 849681, 3 interactors |
DIPi | DIP-11502N |
IntActi | P0AB65, 4 interactors |
STRINGi | 511145.b0968 |
Proteomic databases
jPOSTi | P0AB65 |
PaxDbi | P0AB65 |
PRIDEi | P0AB65 |
Genome annotation databases
EnsemblBacteriai | AAC74054; AAC74054; b0968 BAA35733; BAA35733; BAA35733 |
GeneIDi | 58346666 945304 |
KEGGi | ecj:JW5131 eco:b0968 |
PATRICi | fig|1411691.4.peg.1305 |
Organism-specific databases
EchoBASEi | EB3490 |
Phylogenomic databases
eggNOGi | COG1254, Bacteria |
HOGENOMi | CLU_141932_1_2_6 |
InParanoidi | P0AB65 |
PhylomeDBi | P0AB65 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6502-MONOMER MetaCyc:G6502-MONOMER |
BRENDAi | 3.6.1.7, 2026 |
SABIO-RKi | P0AB65 |
Miscellaneous databases
EvolutionaryTracei | P0AB65 |
PROi | PR:P0AB65 |
Family and domain databases
HAMAPi | MF_01450, Acylphosphatase_entero, 1 hit |
InterProi | View protein in InterPro IPR020456, Acylphosphatase IPR001792, Acylphosphatase-like_dom IPR036046, Acylphosphatase-like_dom_sf IPR028627, Acylphosphatase_bac IPR017968, Acylphosphatase_CS |
PANTHERi | PTHR47268, PTHR47268, 1 hit |
Pfami | View protein in Pfam PF00708, Acylphosphatase, 1 hit |
PRINTSi | PR00112, ACYLPHPHTASE |
SUPFAMi | SSF54975, SSF54975, 1 hit |
PROSITEi | View protein in PROSITE PS00150, ACYLPHOSPHATASE_1, 1 hit PS00151, ACYLPHOSPHATASE_2, 1 hit PS51160, ACYLPHOSPHATASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACYP_ECOLI | |
Accessioni | P0AB65Primary (citable) accession number: P0AB65 Secondary accession number(s): P75877, Q9R7Q1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families