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Protein

Acylphosphatase

Gene

yccX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Has a considerably reduced catalytic efficiency compared to other mesophilic acylphosphatases. Shows a considerable resistance against urea denaturation since the full enzymatic activity is maintained in the presence of urea concentrations approaching 6.0 M and that only a slight decreae of 10-15 % was observed with higher urea concentrations.1 Publication

Catalytic activityi

An acylphosphate + H2O = a carboxylate + phosphate.UniRule annotation1 Publication

Kineticsi

  1. KM=0.54 mM for benzoylphosphate1 Publication

    pH dependencei

    Optimum pH is 5.2-6.5.1 Publication

    Temperature dependencei

    Thermostable. Retains 85-90 % of its activity after 3 hours of incubation at 90 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei20UniRule annotation1
    Active sitei38UniRule annotation1

    GO - Molecular functioni

    • acylphosphatase activity Source: EcoCyc

    GO - Biological processi

    • response to heat Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6502-MONOMER
    MetaCyc:G6502-MONOMER
    BRENDAi3.6.1.7 2026
    SABIO-RKiP0AB65

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acylphosphatase2 PublicationsUniRule annotation (EC:3.6.1.7UniRule annotation1 Publication)
    Alternative name(s):
    Acylphosphate phosphohydrolaseUniRule annotationCurated
    Gene namesi
    Name:yccXUniRule annotation
    Ordered Locus Names:b0968, JW5131
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13726 yccX

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi5C → A: No change in secondary structure. Nearly identical enzymatic properties. Loss of activity in 8.0 M urea. Reduced thermodynamic activity. Inactivated after 3 hours of incubation at 90 degrees Celsius. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001585531 – 92AcylphosphataseAdd BLAST92

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi5 ↔ 49UniRule annotation1 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP0AB65
    PRIDEiP0AB65

    Interactioni

    Protein-protein interaction databases

    BioGridi4260041, 6 interactors
    DIPiDIP-11502N
    IntActiP0AB65, 4 interactors
    STRINGi316385.ECDH10B_1038

    Structurei

    Secondary structure

    192
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 13Combined sources9
    Turni15 – 18Combined sources4
    Helixi23 – 30Combined sources8
    Beta strandi35 – 38Combined sources4
    Beta strandi40 – 42Combined sources3
    Beta strandi44 – 48Combined sources5
    Helixi52 – 64Combined sources13
    Beta strandi70 – 80Combined sources11

    3D structure databases

    ProteinModelPortaliP0AB65
    SMRiP0AB65
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AB65

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 92Acylphosphatase-likeUniRule annotationAdd BLAST88

    Sequence similaritiesi

    Belongs to the acylphosphatase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105VSY Bacteria
    COG1254 LUCA
    HOGENOMiHOG000292686
    InParanoidiP0AB65
    KOiK01512
    OMAiRGWVRNR
    PhylomeDBiP0AB65

    Family and domain databases

    HAMAPiMF_01450 Acylphosphatase_entero, 1 hit
    InterProiView protein in InterPro
    IPR020456 Acylphosphatase
    IPR001792 Acylphosphatase-like_dom
    IPR036046 Acylphosphatase-like_dom_sf
    IPR028627 Acylphosphatase_bac
    IPR017968 Acylphosphatase_CS
    PANTHERiPTHR10029 PTHR10029, 1 hit
    PfamiView protein in Pfam
    PF00708 Acylphosphatase, 1 hit
    PRINTSiPR00112 ACYLPHPHTASE
    SUPFAMiSSF54975 SSF54975, 1 hit
    PROSITEiView protein in PROSITE
    PS00150 ACYLPHOSPHATASE_1, 1 hit
    PS00151 ACYLPHOSPHATASE_2, 1 hit
    PS51160 ACYLPHOSPHATASE_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0AB65-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKVCIIAWV YGRVQGVGFR YTTQYEAKRL GLTGYAKNLD DGSVEVVACG
    60 70 80 90
    EEGQVEKLMQ WLKSGGPRSA RVERVLSEPH HPSGELTDFR IR
    Length:92
    Mass (Da):10,300
    Last modified:November 8, 2005 - v1
    Checksum:iC39D3D145598D873
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74054.1
    AP009048 Genomic DNA Translation: BAA35733.2
    PIRiG64837
    RefSeqiNP_415488.1, NC_000913.3
    WP_000048252.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74054; AAC74054; b0968
    BAA35733; BAA35733; BAA35733
    GeneIDi945304
    KEGGiecj:JW5131
    eco:b0968
    PATRICifig|1411691.4.peg.1305

    Similar proteinsi

    Entry informationi

    Entry nameiACYP_ECOLI
    AccessioniPrimary (citable) accession number: P0AB65
    Secondary accession number(s): P75877, Q9R7Q1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 8, 2005
    Last modified: March 28, 2018
    This is version 100 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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