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Protein

Sulfurtransferase TusE

Gene

tusE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Could accept sulfur from TusD.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108Cysteine persulfide intermediate1

GO - Molecular functioni

  • sulfur carrier activity Source: EcoCyc
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12876-MONOMER
MetaCyc:EG12876-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfurtransferase TusE (EC:2.8.1.-)
Alternative name(s):
tRNA 2-thiouridine synthesizing protein E
Gene namesi
Name:tusE
Synonyms:yccK
Ordered Locus Names:b0969, JW0952
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12876 tusE

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687891 – 109Sulfurtransferase TusEAdd BLAST109

Proteomic databases

PaxDbiP0AB18
PRIDEiP0AB18

Interactioni

Subunit structurei

Interacts with the TusBCD complex. Interacts with MnmA.1 Publication

Protein-protein interaction databases

BioGridi4260042, 10 interactors
ComplexPortaliCPX-2144 TusBCDE complex
CPX-2145 TusE-MnmA complex
DIPiDIP-48154N
IntActiP0AB18, 11 interactors
STRINGi316385.ECDH10B_1039

Structurei

3D structure databases

ProteinModelPortaliP0AB18
SMRiP0AB18
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrC/TusE family.Curated

Phylogenomic databases

eggNOGiENOG4108Z0T Bacteria
COG2920 LUCA
HOGENOMiHOG000279490
InParanoidiP0AB18
KOiK11179
OMAiLPKPTNC
PhylomeDBiP0AB18

Family and domain databases

InterProiView protein in InterPro
IPR025526 DsrC-like_dom_sf
IPR007453 DsrC/TusE
PANTHERiPTHR37010 PTHR37010, 1 hit
PfamiView protein in Pfam
PF04358 DsrC, 1 hit
PIRSFiPIRSF006223 DsrC_TusE, 1 hit
SUPFAMiSSF69721 SSF69721, 1 hit
TIGRFAMsiTIGR03342 dsrC_tusE_dsvC, 1 hit

Sequencei

Sequence statusi: Complete.

P0AB18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIFEGKEIE TDTEGYLKES SQWSEPLAVV IAENEGISLS PEHWEVVRFV
60 70 80 90 100
RDFYLEFNTS PAIRMLVKAM ANKFGEEKGN SRYLYRLFPK GPAKQATKIA

GLPKPVKCI
Length:109
Mass (Da):12,410
Last modified:October 11, 2005 - v1
Checksum:iFDB0CFF89114EBE7
GO

Sequence cautioni

The sequence X00547 differs from that shown. Reason: Frameshift at position 24.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74055.2
AP009048 Genomic DNA Translation: BAA35734.1
X00547 Genomic DNA No translation available.
PIRiH64837
RefSeqiNP_415489.4, NC_000913.3
WP_000904442.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74055; AAC74055; b0969
BAA35734; BAA35734; BAA35734
GeneIDi945023
KEGGiecj:JW0952
eco:b0969
PATRICifig|1411691.4.peg.1304

Similar proteinsi

Entry informationi

Entry nameiTUSE_ECOLI
AccessioniPrimary (citable) accession number: P0AB18
Secondary accession number(s): P45572, P75878
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 20, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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