UniProtKB - P0AAP1 (DGCC_ECOLI)
Protein
Probable diguanylate cyclase DgcC
Gene
dgcC
Organism
Escherichia coli (strain K12)
Status
Functioni
A probable diguanylate cyclase. The last member of a cascade of expressed proteins, its expression requires DgcM. DgcC production induces biosynthesis of cellulose in some E.coli isolates, but not in K12 strains. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.2 Publications
Catalytic activityi
- EC:2.7.7.651 Publication
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.Curated
: 3',5'-cyclic di-GMP biosynthesis Pathwayi
This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 248 | MagnesiumBy similarity | 1 | |
Metal bindingi | 249 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Sitei | 253 | Transition state stabilizerSequence analysis | 1 | |
Binding sitei | 256 | SubstrateBy similarity | 1 | |
Binding sitei | 265 | SubstrateBy similarity | 1 | |
Metal bindingi | 291 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- diguanylate cyclase activity Source: EcoCyc
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cell adhesion involved in single-species biofilm formation Source: GO_Central
- negative regulation of bacterial-type flagellum-dependent cell motility Source: GO_Central
Keywordsi
Molecular function | Transferase |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11257-MONOMER MetaCyc:EG11257-MONOMER |
UniPathwayi | UPA00599 |
Protein family/group databases
TCDBi | 9.B.34.1.2, the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dgcC1 Publication Synonyms:adrA, yaiC Ordered Locus Names:b0385, JW0376 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane Curated; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 46 – 66 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 68 – 88 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 112 – 132 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 143 – 163 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 171 – 191 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: GO_Central
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 291 – 292 | DE → AA: No c-di-GMP produced in an overexpressing strain. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168588 | 1 – 371 | Probable diguanylate cyclase DgcCAdd BLAST | 371 |
Proteomic databases
PaxDbi | P0AAP1 |
PRIDEi | P0AAP1 |
Expressioni
Inductioni
Expressed at 28 degrees Celsius in late stationary phase, more highly expressed on plates than in liquid medium. Expression is RpoS-, CsgD- and DgcM-dependent.3 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262033, 19 interactors |
STRINGi | 511145.b0385 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 240 – 371 | GGDEFPROSITE-ProRule annotationAdd BLAST | 132 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3706, Bacteria |
HOGENOMi | CLU_000445_11_1_6 |
InParanoidi | P0AAP1 |
PhylomeDBi | P0AAP1 |
Family and domain databases
CDDi | cd01949, GGDEF, 1 hit |
Gene3Di | 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR000160, GGDEF_dom IPR007894, MASE2 IPR029787, Nucleotide_cyclase IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00990, GGDEF, 1 hit PF05230, MASE2, 1 hit |
SMARTi | View protein in SMART SM00267, GGDEF, 1 hit |
SUPFAMi | SSF55073, SSF55073, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit |
PROSITEi | View protein in PROSITE PS50887, GGDEF, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AAP1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFPKIMNDEN FFKKAAAHGE EPPLTPQNEH QRSGLRFARR VRLPRAVGLA
60 70 80 90 100
GMFLPIASTL VSHPPPGWWW LVLVGWAFVW PHLAWQIASR AVDPLSREIY
110 120 130 140 150
NLKTDAVLAG MWVGVMGVNV LPSTAMLMIM CLNLMGAGGP RLFVAGLVLM
160 170 180 190 200
VVSCLVTLEL TGITVSFNSA PLEWWLSLPI IVIYPLLFGW VSYQTATKLA
210 220 230 240 250
EHKRRLQVMS TRDGMTGVYN RRHWETMLRN EFDNCRRHNR DATLLIIDID
260 270 280 290 300
HFKSINDTWG HDVGDEAIVA LTRQLQITLR GSDVIGRFGG DEFAVIMSGT
310 320 330 340 350
PAESAITAML RVHEGLNTLR LPNTPQVTLR ISVGVAPLNP QMSHYREWLK
360 370
SADLALYKAK KAGRNRTEVA A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73857 Genomic DNA Translation: AAB18109.1 U00096 Genomic DNA Translation: AAC73488.1 AP009048 Genomic DNA Translation: BAE76166.1 M13345 Genomic DNA Translation: AAA83895.1 |
PIRi | A64767 |
RefSeqi | NP_414919.1, NC_000913.3 WP_000484048.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73488; AAC73488; b0385 BAE76166; BAE76166; BAE76166 |
GeneIDi | 58460372 945037 |
KEGGi | ecj:JW0376 eco:b0385 |
PATRICi | fig|1411691.4.peg.1893 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73857 Genomic DNA Translation: AAB18109.1 U00096 Genomic DNA Translation: AAC73488.1 AP009048 Genomic DNA Translation: BAE76166.1 M13345 Genomic DNA Translation: AAA83895.1 |
PIRi | A64767 |
RefSeqi | NP_414919.1, NC_000913.3 WP_000484048.1, NZ_STEB01000007.1 |
3D structure databases
SMRi | P0AAP1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262033, 19 interactors |
STRINGi | 511145.b0385 |
Protein family/group databases
TCDBi | 9.B.34.1.2, the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family |
Proteomic databases
PaxDbi | P0AAP1 |
PRIDEi | P0AAP1 |
Genome annotation databases
EnsemblBacteriai | AAC73488; AAC73488; b0385 BAE76166; BAE76166; BAE76166 |
GeneIDi | 58460372 945037 |
KEGGi | ecj:JW0376 eco:b0385 |
PATRICi | fig|1411691.4.peg.1893 |
Organism-specific databases
EchoBASEi | EB1237 |
Phylogenomic databases
eggNOGi | COG3706, Bacteria |
HOGENOMi | CLU_000445_11_1_6 |
InParanoidi | P0AAP1 |
PhylomeDBi | P0AAP1 |
Enzyme and pathway databases
UniPathwayi | UPA00599 |
BioCyci | EcoCyc:EG11257-MONOMER MetaCyc:EG11257-MONOMER |
Miscellaneous databases
PROi | PR:P0AAP1 |
Family and domain databases
CDDi | cd01949, GGDEF, 1 hit |
Gene3Di | 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR000160, GGDEF_dom IPR007894, MASE2 IPR029787, Nucleotide_cyclase IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00990, GGDEF, 1 hit PF05230, MASE2, 1 hit |
SMARTi | View protein in SMART SM00267, GGDEF, 1 hit |
SUPFAMi | SSF55073, SSF55073, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit |
PROSITEi | View protein in PROSITE PS50887, GGDEF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DGCC_ECOLI | |
Accessioni | P0AAP1Primary (citable) accession number: P0AAP1 Secondary accession number(s): P21830, P77719, Q2MC40 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | February 10, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways