dgcC

Functionⁱ

A probable diguanylate cyclase. The last member of a cascade of expressed proteins, its expression requires DgcM. DgcC production induces biosynthesis of cellulose in some E.coli isolates, but not in K12 strains. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.2 Publications

Catalytic activityⁱ

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.1 Publication

Cofactorⁱ

Mg²⁺By similarityNote: Binds 1 Mg²⁺ ion per monomer.Curated

Pathwayⁱ: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	248	MagnesiumBy similarity	1
Metal bindingⁱ	249	Magnesium; via carbonyl oxygenBy similarity	1
Siteⁱ	253	Transition state stabilizerSequence analysis	1
Binding siteⁱ	256	SubstrateBy similarity	1
Binding siteⁱ	265	SubstrateBy similarity	1
Metal bindingⁱ	291	MagnesiumBy similarity	1

GO - Molecular functionⁱ

diguanylate cyclase activity
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 19707751
- 20576684
GTP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Ligand	GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11257-MONOMER MetaCyc:EG11257-MONOMER
UniPathwayⁱ	UPA00599

Protein family/group databases

TCDBⁱ	9.B.34.1.2 the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family

TCDBⁱ

9.B.34.1.2 the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable diguanylate cyclase DgcCCurated (EC:2.7.7.651 Publication) Short name: DGC
Gene namesⁱ	Name:dgcC1 Publication Synonyms:adrA, yaiC Ordered Locus Names:b0385, JW0376
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11257 adrA

EcoGeneⁱ

EG11257 adrA

Subcellular locationⁱ

Cell inner membrane Curated; Multi-pass membrane protein Sequence analysis

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	46 – 66	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	68 – 88	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	112 – 132	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	143 – 163	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	171 – 191	HelicalSequence analysisAdd BLAST	21

GO - Cellular componentⁱ

integral component of membrane Source: UniProtKB-KW
plasma membrane Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	291 – 292	DE → AA: No c-di-GMP produced in an overexpressing strain. 1 Publication		2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000168588	1 – 371	Probable diguanylate cyclase DgcCAdd BLAST		371

Proteomic databases

PaxDbⁱ	P0AAP1
PRIDEⁱ	P0AAP1

Expressionⁱ

Inductionⁱ

Expressed at 28 degrees Celsius in late stationary phase, more highly expressed on plates than in liquid medium. Expression is RpoS-, CsgD- and DgcM-dependent.3 Publications

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4262033, 19 interactors
STRINGⁱ	316385.ECDH10B_0342

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AAP1
SMRⁱ	P0AAP1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	240 – 371	GGDEFPROSITE-ProRule annotationAdd BLAST		132

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105BZU Bacteria ENOG410XNMH LUCA
HOGENOMⁱ	HOG000117511
InParanoidⁱ	P0AAP1
KEGG Orthology (KO) More...KOⁱ	K18968
OMAⁱ	HGAWKDL
PhylomeDBⁱ	P0AAP1

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01949 GGDEF, 1 hit
InterProⁱ	View protein in InterPro IPR000160 GGDEF_dom IPR007894 MASE2 IPR029787 Nucleotide_cyclase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00990 GGDEF, 1 hit PF05230 MASE2, 1 hit
SMARTⁱ	View protein in SMART SM00267 GGDEF, 1 hit
SUPFAMⁱ	SSF55073 SSF55073, 1 hit
TIGRFAMsⁱ	TIGR00254 GGDEF, 1 hit
PROSITEⁱ	View protein in PROSITE PS50887 GGDEF, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AAP1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFPKIMNDEN FFKKAAAHGE EPPLTPQNEH QRSGLRFARR VRLPRAVGLA 
        60         70         80         90        100
GMFLPIASTL VSHPPPGWWW LVLVGWAFVW PHLAWQIASR AVDPLSREIY 
       110        120        130        140        150
NLKTDAVLAG MWVGVMGVNV LPSTAMLMIM CLNLMGAGGP RLFVAGLVLM 
       160        170        180        190        200
VVSCLVTLEL TGITVSFNSA PLEWWLSLPI IVIYPLLFGW VSYQTATKLA 
       210        220        230        240        250
EHKRRLQVMS TRDGMTGVYN RRHWETMLRN EFDNCRRHNR DATLLIIDID 
       260        270        280        290        300
HFKSINDTWG HDVGDEAIVA LTRQLQITLR GSDVIGRFGG DEFAVIMSGT 
       310        320        330        340        350
PAESAITAML RVHEGLNTLR LPNTPQVTLR ISVGVAPLNP QMSHYREWLK 
       360        370 
SADLALYKAK KAGRNRTEVA A

Length:371

Mass (Da):41,537

Last modified:October 11, 2005 - v1

Checksum:ⁱ1F5111C43481F32B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U73857 Genomic DNA Translation: AAB18109.1 U00096 Genomic DNA Translation: AAC73488.1 AP009048 Genomic DNA Translation: BAE76166.1 M13345 Genomic DNA Translation: AAA83895.1
PIRⁱ	A64767
NCBI Reference Sequences More...RefSeqⁱ	NP_414919.1, NC_000913.3 WP_000484048.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73488; AAC73488; b0385 BAE76166; BAE76166; BAE76166
GeneIDⁱ	945037
KEGGⁱ	ecj:JW0376 eco:b0385
PATRICⁱ	fig\|1411691.4.peg.1893

Protein	Similar proteins		Species	Score	Length
P0AAP1	Probable diguanylate cyclase DgcC		ECOL6		371
Protein YaiC		Klebsiella pneumoniae IS22		371
Diguanylate cyclase		ECOLX		371
Diguanylate cyclase domain protein		Escherichia coli 907672		371
Diguanylate cyclase (GGDEF) domain-containing protein		Escherichia coli KTE108		371
+74

Protein	Similar proteins		Species	Score	Length
P0AAP1	Probable diguanylate cyclase DgcC		ECOL6		371
Protein YaiC		Klebsiella pneumoniae IS22		371
Diguanylate cyclase		ECOLX		371
Diguanylate cyclase domain protein		Escherichia coli 907672		371
Diguanylate cyclase (GGDEF) domain-containing protein		Escherichia coli KTE108		371
+546

Protein	Similar proteins		Species	Score	Length
P0AAP1	Probable diguanylate cyclase DgcC		ECOL6		371
Protein YaiC		Klebsiella pneumoniae IS22		371
Diguanylate cyclase		ECOLX		371
Diguanylate cyclase domain protein		Escherichia coli 907672		371
Diguanylate cyclase (GGDEF) domain-containing protein		Escherichia coli KTE108		371
+1186

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U73857 Genomic DNA Translation: AAB18109.1 U00096 Genomic DNA Translation: AAC73488.1 AP009048 Genomic DNA Translation: BAE76166.1 M13345 Genomic DNA Translation: AAA83895.1
PIRⁱ	A64767
NCBI Reference Sequences More...RefSeqⁱ	NP_414919.1, NC_000913.3 WP_000484048.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0AAP1
SMRⁱ	P0AAP1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262033, 19 interactors
STRINGⁱ	316385.ECDH10B_0342

Protein family/group databases

TCDBⁱ	9.B.34.1.2 the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family

TCDBⁱ

9.B.34.1.2 the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family

Proteomic databases

PaxDbⁱ	P0AAP1
PRIDEⁱ	P0AAP1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73488; AAC73488; b0385 BAE76166; BAE76166; BAE76166
GeneIDⁱ	945037
KEGGⁱ	ecj:JW0376 eco:b0385
PATRICⁱ	fig\|1411691.4.peg.1893

Organism-specific databases

EchoBASEⁱ	EB1237
EcoGeneⁱ	EG11257 adrA

Phylogenomic databases

eggNOGⁱ	ENOG4105BZU Bacteria ENOG410XNMH LUCA
HOGENOMⁱ	HOG000117511
InParanoidⁱ	P0AAP1
KEGG Orthology (KO) More...KOⁱ	K18968
OMAⁱ	HGAWKDL
PhylomeDBⁱ	P0AAP1

Enzyme and pathway databases

UniPathwayⁱ	UPA00599
BioCycⁱ	EcoCyc:EG11257-MONOMER MetaCyc:EG11257-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AAP1

PROⁱ

PR:P0AAP1

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01949 GGDEF, 1 hit
InterProⁱ	View protein in InterPro IPR000160 GGDEF_dom IPR007894 MASE2 IPR029787 Nucleotide_cyclase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00990 GGDEF, 1 hit PF05230 MASE2, 1 hit
SMARTⁱ	View protein in SMART SM00267 GGDEF, 1 hit
SUPFAMⁱ	SSF55073 SSF55073, 1 hit
TIGRFAMsⁱ	TIGR00254 GGDEF, 1 hit
PROSITEⁱ	View protein in PROSITE PS50887 GGDEF, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DGCC_ECOLI
Accessionⁱ	P0AAP1Primary (citable) accession number: P0AAP1 Secondary accession number(s): P21830, P77719, Q2MC40
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
	Last sequence update:	October 11, 2005
	Last modified:	March 28, 2018
	This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AAP1 (DGCC_ECOLI)

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Probable diguanylate cyclase DgcC

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: 3',5'-cyclic di-GMP biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: 3',5'-cyclic di-GMP biosynthesis

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ