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Protein

Probable diguanylate cyclase DgcC

Gene

dgcC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A probable diguanylate cyclase. The last member of a cascade of expressed proteins, its expression requires DgcM. DgcC production induces biosynthesis of cellulose in some E.coli isolates, but not in K12 strains. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.2 Publications

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.Curated

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi248MagnesiumBy similarity1
Metal bindingi249Magnesium; via carbonyl oxygenBy similarity1
Sitei253Transition state stabilizerSequence analysis1
Binding sitei256SubstrateBy similarity1
Binding sitei265SubstrateBy similarity1
Metal bindingi291MagnesiumBy similarity1

GO - Molecular functioni

  • diguanylate cyclase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11257-MONOMER
MetaCyc:EG11257-MONOMER
UniPathwayiUPA00599

Protein family/group databases

TCDBi9.B.34.1.2 the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase DgcCCurated (EC:2.7.7.651 Publication)
Short name:
DGC
Gene namesi
Name:dgcC1 Publication
Synonyms:adrA, yaiC
Ordered Locus Names:b0385, JW0376
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11257 adrA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Transmembranei68 – 88HelicalSequence analysisAdd BLAST21
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291 – 292DE → AA: No c-di-GMP produced in an overexpressing strain. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685881 – 371Probable diguanylate cyclase DgcCAdd BLAST371

Proteomic databases

PaxDbiP0AAP1
PRIDEiP0AAP1

Expressioni

Inductioni

Expressed at 28 degrees Celsius in late stationary phase, more highly expressed on plates than in liquid medium. Expression is RpoS-, CsgD- and DgcM-dependent.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262033, 19 interactors
STRINGi316385.ECDH10B_0342

Structurei

3D structure databases

ProteinModelPortaliP0AAP1
SMRiP0AAP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini240 – 371GGDEFPROSITE-ProRule annotationAdd BLAST132

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000117511
InParanoidiP0AAP1
KOiK18968
OMAiHGAWKDL
PhylomeDBiP0AAP1

Family and domain databases

CDDicd01949 GGDEF, 1 hit
InterProiView protein in InterPro
IPR000160 GGDEF_dom
IPR007894 MASE2
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00990 GGDEF, 1 hit
PF05230 MASE2, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

P0AAP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPKIMNDEN FFKKAAAHGE EPPLTPQNEH QRSGLRFARR VRLPRAVGLA
60 70 80 90 100
GMFLPIASTL VSHPPPGWWW LVLVGWAFVW PHLAWQIASR AVDPLSREIY
110 120 130 140 150
NLKTDAVLAG MWVGVMGVNV LPSTAMLMIM CLNLMGAGGP RLFVAGLVLM
160 170 180 190 200
VVSCLVTLEL TGITVSFNSA PLEWWLSLPI IVIYPLLFGW VSYQTATKLA
210 220 230 240 250
EHKRRLQVMS TRDGMTGVYN RRHWETMLRN EFDNCRRHNR DATLLIIDID
260 270 280 290 300
HFKSINDTWG HDVGDEAIVA LTRQLQITLR GSDVIGRFGG DEFAVIMSGT
310 320 330 340 350
PAESAITAML RVHEGLNTLR LPNTPQVTLR ISVGVAPLNP QMSHYREWLK
360 370
SADLALYKAK KAGRNRTEVA A
Length:371
Mass (Da):41,537
Last modified:October 11, 2005 - v1
Checksum:i1F5111C43481F32B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18109.1
U00096 Genomic DNA Translation: AAC73488.1
AP009048 Genomic DNA Translation: BAE76166.1
M13345 Genomic DNA Translation: AAA83895.1
PIRiA64767
RefSeqiNP_414919.1, NC_000913.3
WP_000484048.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73488; AAC73488; b0385
BAE76166; BAE76166; BAE76166
GeneIDi945037
KEGGiecj:JW0376
eco:b0385
PATRICifig|1411691.4.peg.1893

Similar proteinsi

Entry informationi

Entry nameiDGCC_ECOLI
AccessioniPrimary (citable) accession number: P0AAP1
Secondary accession number(s): P21830, P77719, Q2MC40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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