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Entry version 109 (02 Dec 2020)
Sequence version 1 (11 Oct 2005)
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Protein

Ferredoxin-type protein NapF

Gene

napF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Could be involved in the maturation of NapA, the catalytic subunit of the periplasmic nitrate reductase, before its export into the periplasm (PubMed:17074894). Is not involved in the electron transfer from menaquinol or ubiquinol to the periplasmic nitrate reductase (PubMed:11967083).2 Publications

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 3 [4Fe-4S] cluster.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi37Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi40Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi43Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi69Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi72Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi75Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi141Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi144Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi147Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)UniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • response to oxidative stress Source: EcoCyc

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:NAPF-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
3.D.11.1.1, the periplasmic nitrate reductase complex (nap) complex family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ferredoxin-type protein NapFUniRule annotationCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:napFUniRule annotation
Synonyms:yojG
Ordered Locus Names:b2208, JW2196
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Deletion of the gene does not decrease the rate of electron transfer from physiological substrates to the Nap complex, but it has a significant effect on cell energetics.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi7 – 8RR → AA: No change in activity. 1 Publication2
Mutagenesisi24P → A: No change in activity. 1 Publication1
Mutagenesisi25P → A: No change in activity. 1 Publication1
Mutagenesisi26W → Y: No change in activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001592751 – 164Ferredoxin-type protein NapFAdd BLAST164

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0AAL0

PRoteomics IDEntifications database

More...
PRIDEi
P0AAL0

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with the cytoplasmic NapA precursor.

UniRule annotation1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4262219, 35 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2208

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini28 – 574Fe-4S ferredoxin-type 1UniRule annotationAdd BLAST30
Domaini58 – 894Fe-4S ferredoxin-type 2UniRule annotationAdd BLAST32
Domaini132 – 1614Fe-4S ferredoxin-type 3UniRule annotationAdd BLAST30

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NapF family.UniRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1145, Bacteria
COG1149, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_077329_2_1_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0AAL0

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0AAL0

Family and domain databases

Conserved Domains Database

More...
CDDi
cd10564, NapF_like, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02201, NapF, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR017896, 4Fe4S_Fe-S-bd
IPR017900, 4Fe4S_Fe_S_CS
IPR004496, NapF

The PANTHER Classification System

More...
PANTHERi
PTHR24960:SF46, PTHR24960:SF46, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00037, Fer4, 1 hit
PF12800, Fer4_4, 1 hit
PF13187, Fer4_9, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00402, napF, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00198, 4FE4S_FER_1, 3 hits
PS51379, 4FE4S_FER_2, 3 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AAL0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIDASRRGI LTGRWRKASN GIRPPWSGDE SHFLTHCTRC DACINACENN
60 70 80 90 100
ILQRGAGGYP SVNFKNNECS FCYACAQACP ESLFSPRHTR AWDLQFTIGD
110 120 130 140 150
ACLAYQSVEC RRCQDSCEPM AIIFRPTLSG IYQPQLNSQL CNGCGACAAS
160
CPVSAITAEY LHAH
Length:164
Mass (Da):18,047
Last modified:October 11, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3832B3D78805FAC1
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16401.1
U00096 Genomic DNA Translation: AAC75268.1
AP009048 Genomic DNA Translation: BAA15991.1

Protein sequence database of the Protein Information Resource

More...
PIRi
F64990

NCBI Reference Sequences

More...
RefSeqi
NP_416712.1, NC_000913.3
WP_000686723.1, NZ_SSZK01000027.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75268; AAC75268; b2208
BAA15991; BAA15991; BAA15991

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
49583973
946813

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2196
eco:b2208

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.28

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16401.1
U00096 Genomic DNA Translation: AAC75268.1
AP009048 Genomic DNA Translation: BAA15991.1
PIRiF64990
RefSeqiNP_416712.1, NC_000913.3
WP_000686723.1, NZ_SSZK01000027.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4262219, 35 interactors
STRINGi511145.b2208

Protein family/group databases

TCDBi3.D.11.1.1, the periplasmic nitrate reductase complex (nap) complex family

Proteomic databases

PaxDbiP0AAL0
PRIDEiP0AAL0

Genome annotation databases

EnsemblBacteriaiAAC75268; AAC75268; b2208
BAA15991; BAA15991; BAA15991
GeneIDi49583973
946813
KEGGiecj:JW2196
eco:b2208
PATRICifig|1411691.4.peg.28

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1995

Phylogenomic databases

eggNOGiCOG1145, Bacteria
COG1149, Bacteria
HOGENOMiCLU_077329_2_1_6
InParanoidiP0AAL0
PhylomeDBiP0AAL0

Enzyme and pathway databases

BioCyciEcoCyc:NAPF-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P0AAL0

Family and domain databases

CDDicd10564, NapF_like, 1 hit
HAMAPiMF_02201, NapF, 1 hit
InterProiView protein in InterPro
IPR017896, 4Fe4S_Fe-S-bd
IPR017900, 4Fe4S_Fe_S_CS
IPR004496, NapF
PANTHERiPTHR24960:SF46, PTHR24960:SF46, 1 hit
PfamiView protein in Pfam
PF00037, Fer4, 1 hit
PF12800, Fer4_4, 1 hit
PF13187, Fer4_9, 1 hit
TIGRFAMsiTIGR00402, napF, 1 hit
PROSITEiView protein in PROSITE
PS00198, 4FE4S_FER_1, 3 hits
PS51379, 4FE4S_FER_2, 3 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNAPF_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AAL0
Secondary accession number(s): P33939
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: December 2, 2020
This is version 109 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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