UniProtKB - P0AAL0 (NAPF_ECOLI)
Protein
Ferredoxin-type protein NapF
Gene
napF
Organism
Escherichia coli (strain K12)
Status
Functioni
Could be involved in the maturation of NapA, the catalytic subunit of the periplasmic nitrate reductase, before its export into the periplasm (PubMed:17074894). Is not involved in the electron transfer from menaquinol or ubiquinol to the periplasmic nitrate reductase (PubMed:11967083).2 Publications
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 3 [4Fe-4S] cluster.PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 37 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 40 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 43 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 47 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 69 | Iron-sulfur 2 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 72 | Iron-sulfur 2 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 75 | Iron-sulfur 2 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 79 | Iron-sulfur 2 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 141 | Iron-sulfur 3 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 144 | Iron-sulfur 3 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 147 | Iron-sulfur 3 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 151 | Iron-sulfur 3 (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- response to oxidative stress Source: EcoCyc
Keywordsi
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:NAPF-MONOMER |
Protein family/group databases
TCDBi | 3.D.11.1.1, the periplasmic nitrate reductase complex (nap) complex family |
Names & Taxonomyi
Protein namesi | Recommended name: Ferredoxin-type protein NapFUniRule annotationCurated |
Gene namesi | Name:napFUniRule annotation Synonyms:yojG Ordered Locus Names:b2208, JW2196 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication Note: Loosely attached to the inner side of the membrane.1 Publication
GO - Cellular componenti
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Deletion of the gene does not decrease the rate of electron transfer from physiological substrates to the Nap complex, but it has a significant effect on cell energetics.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 – 8 | RR → AA: No change in activity. 1 Publication | 2 | |
Mutagenesisi | 24 | P → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 25 | P → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 26 | W → Y: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159275 | 1 – 164 | Ferredoxin-type protein NapFAdd BLAST | 164 |
Proteomic databases
PaxDbi | P0AAL0 |
PRIDEi | P0AAL0 |
Interactioni
Subunit structurei
Interacts with the cytoplasmic NapA precursor.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4262219, 35 interactors |
STRINGi | 511145.b2208 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 57 | 4Fe-4S ferredoxin-type 1UniRule annotationAdd BLAST | 30 | |
Domaini | 58 – 89 | 4Fe-4S ferredoxin-type 2UniRule annotationAdd BLAST | 32 | |
Domaini | 132 – 161 | 4Fe-4S ferredoxin-type 3UniRule annotationAdd BLAST | 30 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1145, Bacteria COG1149, Bacteria |
HOGENOMi | CLU_077329_2_1_6 |
InParanoidi | P0AAL0 |
PhylomeDBi | P0AAL0 |
Family and domain databases
CDDi | cd10564, NapF_like, 1 hit |
HAMAPi | MF_02201, NapF, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR004496, NapF |
PANTHERi | PTHR24960:SF46, PTHR24960:SF46, 1 hit |
Pfami | View protein in Pfam PF00037, Fer4, 1 hit PF12800, Fer4_4, 1 hit PF13187, Fer4_9, 1 hit |
TIGRFAMsi | TIGR00402, napF, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 3 hits PS51379, 4FE4S_FER_2, 3 hits |
i Sequence
Sequence statusi: Complete.
P0AAL0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIDASRRGI LTGRWRKASN GIRPPWSGDE SHFLTHCTRC DACINACENN
60 70 80 90 100
ILQRGAGGYP SVNFKNNECS FCYACAQACP ESLFSPRHTR AWDLQFTIGD
110 120 130 140 150
ACLAYQSVEC RRCQDSCEPM AIIFRPTLSG IYQPQLNSQL CNGCGACAAS
160
CPVSAITAEY LHAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16401.1 U00096 Genomic DNA Translation: AAC75268.1 AP009048 Genomic DNA Translation: BAA15991.1 |
PIRi | F64990 |
RefSeqi | NP_416712.1, NC_000913.3 WP_000686723.1, NZ_SSZK01000027.1 |
Genome annotation databases
EnsemblBacteriai | AAC75268; AAC75268; b2208 BAA15991; BAA15991; BAA15991 |
GeneIDi | 49583973 946813 |
KEGGi | ecj:JW2196 eco:b2208 |
PATRICi | fig|1411691.4.peg.28 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16401.1 U00096 Genomic DNA Translation: AAC75268.1 AP009048 Genomic DNA Translation: BAA15991.1 |
PIRi | F64990 |
RefSeqi | NP_416712.1, NC_000913.3 WP_000686723.1, NZ_SSZK01000027.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262219, 35 interactors |
STRINGi | 511145.b2208 |
Protein family/group databases
TCDBi | 3.D.11.1.1, the periplasmic nitrate reductase complex (nap) complex family |
Proteomic databases
PaxDbi | P0AAL0 |
PRIDEi | P0AAL0 |
Genome annotation databases
EnsemblBacteriai | AAC75268; AAC75268; b2208 BAA15991; BAA15991; BAA15991 |
GeneIDi | 49583973 946813 |
KEGGi | ecj:JW2196 eco:b2208 |
PATRICi | fig|1411691.4.peg.28 |
Organism-specific databases
EchoBASEi | EB1995 |
Phylogenomic databases
eggNOGi | COG1145, Bacteria COG1149, Bacteria |
HOGENOMi | CLU_077329_2_1_6 |
InParanoidi | P0AAL0 |
PhylomeDBi | P0AAL0 |
Enzyme and pathway databases
BioCyci | EcoCyc:NAPF-MONOMER |
Miscellaneous databases
PROi | PR:P0AAL0 |
Family and domain databases
CDDi | cd10564, NapF_like, 1 hit |
HAMAPi | MF_02201, NapF, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR004496, NapF |
PANTHERi | PTHR24960:SF46, PTHR24960:SF46, 1 hit |
Pfami | View protein in Pfam PF00037, Fer4, 1 hit PF12800, Fer4_4, 1 hit PF13187, Fer4_9, 1 hit |
TIGRFAMsi | TIGR00402, napF, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 3 hits PS51379, 4FE4S_FER_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NAPF_ECOLI | |
Accessioni | P0AAL0Primary (citable) accession number: P0AAL0 Secondary accession number(s): P33939 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families