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UniProtKB - P0AAG5 (MDLB_ECOLI)
Protein
Multidrug resistance-like ATP-binding protein MdlB
Gene
mdlB
Organism
Escherichia coli (strain K12)
Status
Functioni
Caution
Was originally proposed to be fused with MdlA.1 Publication
Catalytic activityi
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2). EC:7.6.2.2
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 374 – 381 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type xenobiotic transporter activity Source: UniProtKB-EC
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: EcoCyc
GO - Biological processi
- transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MDLB-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.106.13, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mdlB Synonyms:mdl Ordered Locus Names:b0449, JW5061 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 25 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 26 – 46 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 47 – 62 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 63 – 83 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 84 – 140 | CytoplasmicSequence analysisAdd BLAST | 57 | |
Transmembranei | 141 – 161 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 162 – 164 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 165 – 185 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 186 – 254 | CytoplasmicSequence analysisAdd BLAST | 69 | |
Transmembranei | 255 – 275 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 276 – 278 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 279 – 299 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 300 – 593 | CytoplasmicSequence analysisAdd BLAST | 294 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092496 | 1 – 593 | Multidrug resistance-like ATP-binding protein MdlBAdd BLAST | 593 |
Proteomic databases
PaxDbi | P0AAG5 |
PRIDEi | P0AAG5 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261405, 185 interactors |
STRINGi | 511145.b0449 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 310 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 286 | |
Domaini | 341 – 574 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 234 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1132, Bacteria |
HOGENOMi | CLU_000604_84_3_6 |
InParanoidi | P0AAG5 |
OMAi | MSVMMAT |
PhylomeDBi | P0AAG5 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AAG5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRSFSQLWPT LKRLLAYGSP WRKPLGIAVL MMWVAAAAEV SGPLLISYFI
60 70 80 90 100
DNMVAKNNLP LKVVAGLAAA YVGLQLFAAG LHYAQSLLFN RAAVGVVQQL
110 120 130 140 150
RTDVMDAALR QPLSEFDTQP VGQVISRVTN DTEVIRDLYV TVVATVLRSA
160 170 180 190 200
ALVGAMLVAM FSLDWRMALV AIMIFPVVLV VMVIYQRYST PIVRRVRAYL
210 220 230 240 250
ADINDGFNEI INGMSVIQQF RQQARFGERM GEASRSHYMA RMQTLRLDGF
260 270 280 290 300
LLRPLLSLFS SLILCGLLML FGFSASGTIE VGVLYAFISY LGRLNEPLIE
310 320 330 340 350
LTTQQAMLQQ AVVAGERVFE LMDGPRQQYG NDDRPLQSGT IEVDNVSFAY
360 370 380 390 400
RDDNLVLKNI NLSVPSRNFV ALVGHTGSGK STLASLLMGY YPLTEGEIRL
410 420 430 440 450
DGRPLSSLSH SALRQGVAMV QQDPVVLADT FLANVTLGRD ISEERVWQAL
460 470 480 490 500
ETVQLAELAR SMSDGIYTPL GEQGNNLSVG QKQLLALARV LVETPQILIL
510 520 530 540 550
DEATASIDSG TEQAIQHALA AVREHTTLVV IAHRLSTIVD ADTILVLHRG
560 570 580 590
QAVEQGTHQQ LLAAQGRYWQ MYQLQLAGEE LAASVREEES LSA
Sequence cautioni
The sequence AAB40205 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAC36870 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 208 – 209 | NE → KQ in AAC36870 (PubMed:7904973).Curated | 2 | |
Sequence conflicti | 336 – 337 | LQ → FT in AAC36870 (PubMed:7904973).Curated | 2 | |
Sequence conflicti | 358 | K → N in AAC36870 (PubMed:7904973).Curated | 1 | |
Sequence conflicti | 411 – 414 | SALR → TRG in AAC36870 (PubMed:7904973).Curated | 4 | |
Sequence conflicti | 457 – 458 | EL → DV in AAC36870 (PubMed:7904973).Curated | 2 | |
Sequence conflicti | 542 | D → A in AAC36870 (PubMed:7904973).Curated | 1 | |
Sequence conflicti | 563 – 593 | AAQGR…ESLSA → RPRDVLADVSTATCGRRAGS QRA in AAC36870 (PubMed:7904973).CuratedAdd BLAST | 31 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08627 Unassigned DNA Translation: AAC36870.1 Different initiation. U82664 Genomic DNA Translation: AAB40205.1 Different initiation. U00096 Genomic DNA Translation: AAC73552.1 AP009048 Genomic DNA Translation: BAE76229.1 |
PIRi | A64775 |
RefSeqi | NP_414983.1, NC_000913.3 WP_001256201.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73552; AAC73552; b0449 BAE76229; BAE76229; BAE76229 |
GeneIDi | 945088 |
KEGGi | ecj:JW5061 eco:b0449 |
PATRICi | fig|1411691.4.peg.1827 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08627 Unassigned DNA Translation: AAC36870.1 Different initiation. U82664 Genomic DNA Translation: AAB40205.1 Different initiation. U00096 Genomic DNA Translation: AAC73552.1 AP009048 Genomic DNA Translation: BAE76229.1 |
PIRi | A64775 |
RefSeqi | NP_414983.1, NC_000913.3 WP_001256201.1, NZ_SSZK01000009.1 |
3D structure databases
AlphaFoldDBi | P0AAG5 |
SMRi | P0AAG5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261405, 185 interactors |
STRINGi | 511145.b0449 |
Protein family/group databases
TCDBi | 3.A.1.106.13, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P0AAG5 |
PRIDEi | P0AAG5 |
Genome annotation databases
EnsemblBacteriai | AAC73552; AAC73552; b0449 BAE76229; BAE76229; BAE76229 |
GeneIDi | 945088 |
KEGGi | ecj:JW5061 eco:b0449 |
PATRICi | fig|1411691.4.peg.1827 |
Organism-specific databases
EchoBASEi | EB4117 |
Phylogenomic databases
eggNOGi | COG1132, Bacteria |
HOGENOMi | CLU_000604_84_3_6 |
InParanoidi | P0AAG5 |
OMAi | MSVMMAT |
PhylomeDBi | P0AAG5 |
Enzyme and pathway databases
BioCyci | EcoCyc:MDLB-MONOMER |
Miscellaneous databases
PROi | PR:P0AAG5 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR027417, P-loop_NTPase IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MDLB_ECOLI | |
Accessioni | P0AAG5Primary (citable) accession number: P0AAG5 Secondary accession number(s): P30751 Q2MBX7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | May 25, 2022 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families