UniProtKB - P0AAF1 (POTE_ECOLI)
Protein
Putrescine transporter PotE
Gene
potE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes both the uptake and excretion of putrescine. The uptake of putrescine is dependent on the membrane potential and the excretion involves putrescine-ornithine antiporter activity.UniRule annotation3 Publications
Activity regulationi
Uptake activity, but not antiporter activity, is inhibited by CCCP and N-ethylmaleimide (NEM). Uptake of putrescine is inhibited by high concentrations of ornithine.2 Publications
Kineticsi
- KM=1.8 µM for putrescine (for uptake activity)1 Publication
- KM=73 µM for putrescine (for antiporter activity)2 Publications
- KM=108 µM for ornithine (for antiporter activity)1 Publication
- Vmax=0.82 nmol/min/mg enzyme for antiporter activity1 Publication
pH dependencei
Optimum pH is 6.5 for uptake activity. Optimum pH is 9.2 for antiporter activity.1 Publication
GO - Molecular functioni
- putrescine:ornithine antiporter activity Source: EcoCyc
- symporter activity Source: EcoCyc
GO - Biological processi
- putrescine transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Antiport, Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:POTE-MONOMER MetaCyc:POTE-MONOMER |
SABIO-RKi | P0AAF1 |
Protein family/group databases
TCDBi | 2.A.3.2.1, the amino acid-polyamine-organocation (apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Putrescine transporter PotEUniRule annotationCuratedAlternative name(s): Putrescine-proton symporter / putrescine-ornithine antiporterUniRule annotationCurated |
Gene namesi | Name:potE2 PublicationsUniRule annotation Ordered Locus Names:b0692, JW0679 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation1 Publication1 Publication; Multi-pass membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 10 – 30 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 31 – 39 | Periplasmic1 Publication | 9 | |
Transmembranei | 40 – 60 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 61 – 90 | Cytoplasmic1 PublicationAdd BLAST | 30 | |
Transmembranei | 91 – 111 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 112 – 113 | Periplasmic1 Publication | 2 | |
Transmembranei | 114 – 134 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 135 – 151 | Cytoplasmic1 PublicationAdd BLAST | 17 | |
Transmembranei | 152 – 172 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 173 – 185 | Periplasmic1 PublicationAdd BLAST | 13 | |
Transmembranei | 186 – 206 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 207 – 224 | Cytoplasmic1 PublicationAdd BLAST | 18 | |
Transmembranei | 225 – 245 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 246 – 275 | Periplasmic1 PublicationAdd BLAST | 30 | |
Transmembranei | 276 – 296 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 297 – 320 | Cytoplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 321 – 341 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 342 – 353 | Periplasmic1 PublicationAdd BLAST | 12 | |
Transmembranei | 354 – 374 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 375 – 386 | Cytoplasmic1 PublicationAdd BLAST | 12 | |
Transmembranei | 387 – 407 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 408 – 409 | Periplasmic1 Publication | 2 | |
Transmembranei | 410 – 430 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 431 – 439 | Cytoplasmic2 Publications | 9 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 62 | C → A or T: Strong decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 62 | C → S: Moderate decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 68 | K → A: Slight decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 77 | E → A, D, N or Q: Strong decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 78 | Y → L: Uptake activity decreases more than excretion activity. 1 Publication | 1 | |
Mutagenesisi | 82 | K → A: Slight decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 90 | Y → L: Uptake activity decreases more than excretion activity. 1 Publication | 1 | |
Mutagenesisi | 92 | Y → L: Moderate decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 201 | W → F, L or Y: Strong decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 207 | E → A, D, N or Q: Lack of both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 210 | C → A: Moderate decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 285 | C → A: Moderate decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 286 | C → A: Moderate decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 292 | W → F, L or Y: Strong decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 301 | K → A: Excretion activity decreases more than uptake activity. 1 Publication | 1 | |
Mutagenesisi | 308 | Y → L: Excretion activity decreases more than uptake activity. 1 Publication | 1 | |
Mutagenesisi | 422 | W → L: Uptake activity decreases more than excretion activity. 1 Publication | 1 | |
Mutagenesisi | 425 | Y → F: Moderate decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 425 | Y → L: Strong decrease in both uptake and excretion activities. 1 Publication | 1 | |
Mutagenesisi | 433 | E → A, D, N or Q: Strong decrease in both uptake and excretion activities. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054250 | 1 – 439 | Putrescine transporter PotEAdd BLAST | 439 |
Proteomic databases
PaxDbi | P0AAF1 |
PRIDEi | P0AAF1 |
Expressioni
Inductioni
Induced at low environmental pH. Part of the speFL-speF-potE operon.1 Publication
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261131, 16 interactors |
IntActi | P0AAF1, 1 interactor |
STRINGi | 511145.b0692 |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid-polyamine-organocation (APC) superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2) family. [View classification]UniRule annotationCurated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0531, Bacteria |
HOGENOMi | CLU_007946_1_0_6 |
InParanoidi | P0AAF1 |
PhylomeDBi | P0AAF1 |
Family and domain databases
HAMAPi | MF_02073, Putrescine_transp, 1 hit |
InterProi | View protein in InterPro IPR002293, AA/rel_permease1 IPR004754, Amino_acid_antiprt IPR027566, Symport/antiport_PotE |
Pfami | View protein in Pfam PF13520, AA_permease_2, 1 hit |
TIGRFAMsi | TIGR00905, 2A0302, 1 hit TIGR04299, antiport_PotE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AAF1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQAKSNKMG VVQLTILTMV NMMGSGIIML PTKLAEVGTI SIISWLVTAV
60 70 80 90 100
GSMALAWAFA KCGMFSRKSG GMGGYAEYAF GKSGNFMANY TYGVSLLIAN
110 120 130 140 150
VAIAISAVGY GTELLGASLS PVQIGLATIG VLWICTVANF GGARITGQIS
160 170 180 190 200
SITVWGVIIP VVGLCIIGWF WFSPTLYVDS WNPHHAPFFS AVGSSIAMTL
210 220 230 240 250
WAFLGLESAC ANTDVVENPE RNVPIAVLGG TLGAAVIYIV STNVIAGIVP
260 270 280 290 300
NMELANSTAP FGLAFAQMFT PEVGKVIMAL MVMSCCGSLL GWQFTIAQVF
310 320 330 340 350
KSSSDEGYFP KIFSRVTKVD APVQGMLTIV IIQSGLALMT ISPSLNSQFN
360 370 380 390 400
VLVNLAVVTN IIPYILSMAA LVIIQKVANV PPSKAKVANF VAFVGAMYSF
410 420 430
YALYSSGEEA MLYGSIVTFL GWTLYGLVSP RFELKNKHG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64495 Genomic DNA Translation: AAA62786.1 U00096 Genomic DNA Translation: AAC73786.1 AP009048 Genomic DNA Translation: BAA35348.1 |
PIRi | B40839 |
RefSeqi | NP_415219.1, NC_000913.3 WP_000075845.1, NZ_STEB01000044.1 |
Genome annotation databases
EnsemblBacteriai | AAC73786; AAC73786; b0692 BAA35348; BAA35348; BAA35348 |
GeneIDi | 945422 |
KEGGi | ecj:JW0679 eco:b0692 |
PATRICi | fig|1411691.4.peg.1584 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64495 Genomic DNA Translation: AAA62786.1 U00096 Genomic DNA Translation: AAC73786.1 AP009048 Genomic DNA Translation: BAA35348.1 |
PIRi | B40839 |
RefSeqi | NP_415219.1, NC_000913.3 WP_000075845.1, NZ_STEB01000044.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261131, 16 interactors |
IntActi | P0AAF1, 1 interactor |
STRINGi | 511145.b0692 |
Protein family/group databases
TCDBi | 2.A.3.2.1, the amino acid-polyamine-organocation (apc) family |
Proteomic databases
PaxDbi | P0AAF1 |
PRIDEi | P0AAF1 |
Genome annotation databases
EnsemblBacteriai | AAC73786; AAC73786; b0692 BAA35348; BAA35348; BAA35348 |
GeneIDi | 945422 |
KEGGi | ecj:JW0679 eco:b0692 |
PATRICi | fig|1411691.4.peg.1584 |
Organism-specific databases
EchoBASEi | EB0746 |
Phylogenomic databases
eggNOGi | COG0531, Bacteria |
HOGENOMi | CLU_007946_1_0_6 |
InParanoidi | P0AAF1 |
PhylomeDBi | P0AAF1 |
Enzyme and pathway databases
BioCyci | EcoCyc:POTE-MONOMER MetaCyc:POTE-MONOMER |
SABIO-RKi | P0AAF1 |
Miscellaneous databases
PROi | PR:P0AAF1 |
Family and domain databases
HAMAPi | MF_02073, Putrescine_transp, 1 hit |
InterProi | View protein in InterPro IPR002293, AA/rel_permease1 IPR004754, Amino_acid_antiprt IPR027566, Symport/antiport_PotE |
Pfami | View protein in Pfam PF13520, AA_permease_2, 1 hit |
TIGRFAMsi | TIGR00905, 2A0302, 1 hit TIGR04299, antiport_PotE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | POTE_ECOLI | |
Accessioni | P0AAF1Primary (citable) accession number: P0AAF1 Secondary accession number(s): P24170 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families