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Protein

Iron-binding protein IscA

Gene

iscA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB.

Miscellaneous

2 iron atoms may bind between dimers.

Cofactori

Fe cationNote: Binds 2 iron ions per dimer. The dimer may bind additional iron ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35IronCurated1
Metal bindingi99IronCurated1
Metal bindingi101IronCurated1

GO - Molecular functioni

GO - Biological processi

  • iron-sulfur cluster assembly Source: UniProtKB
  • protein maturation by iron-sulfur cluster transfer Source: GO_Central

Keywordsi

LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12132-MONOMER
MetaCyc:EG12132-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-binding protein IscA
Alternative name(s):
Iron-sulfur cluster assembly protein
Gene namesi
Name:iscA
Synonyms:yfhF
Ordered Locus Names:b2528, JW2512
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12132 iscA

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35C → S: Decrease of iron binding activity. 1 Publication1
Mutagenesisi99C → S: Loss of iron binding activity. 1 Publication1
Mutagenesisi101C → S: Loss of iron binding activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769971 – 107Iron-binding protein IscAAdd BLAST107

Proteomic databases

EPDiP0AAC8
PaxDbiP0AAC8
PRIDEiP0AAC8

Expressioni

Inductioni

Repressed by IscR.

Interactioni

Subunit structurei

Homodimer; may form tetramers and higher multimers.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
fdxP0A9R44EBI-767026,EBI-767037

Protein-protein interaction databases

BioGridi4260603, 33 interactors
ComplexPortaliCPX-2132 IscA complex
DIPiDIP-35854N
IntActiP0AAC8, 5 interactors
STRINGi316385.ECDH10B_2695

Structurei

Secondary structure

1107
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AAC8
SMRiP0AAC8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAC8

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

eggNOGiENOG4108Z4V Bacteria
COG0316 LUCA
HOGENOMiHOG000228314
InParanoidiP0AAC8
KOiK13628
PhylomeDBiP0AAC8

Family and domain databases

Gene3Di2.60.300.12, 1 hit
HAMAPiMF_01429 Fe_S_insert_IscA, 1 hit
InterProiView protein in InterPro
IPR000361 FeS_biogenesis
IPR016092 FeS_cluster_insertion
IPR017870 FeS_cluster_insertion_CS
IPR035903 HesB-like_dom_sf
IPR011302 IscA_proteobacteria
PfamiView protein in Pfam
PF01521 Fe-S_biosyn, 1 hit
SUPFAMiSSF89360 SSF89360, 1 hit
TIGRFAMsiTIGR02011 IscA, 1 hit
TIGR00049 TIGR00049, 1 hit
PROSITEiView protein in PROSITE
PS01152 HESB, 1 hit

Sequencei

Sequence statusi: Complete.

P0AAC8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSITLSDSAA ARVNTFLANR GKGFGLRLGV RTSGCSGMAY VLEFVDEPTP
60 70 80 90 100
EDIVFEDKGV KVVVDGKSLQ FLDGTQLDFV KEGLNEGFKF TNPNVKDECG

CGESFHV
Length:107
Mass (Da):11,556
Last modified:September 13, 2005 - v1
Checksum:iCBA945AD547E77DD
GO

Sequence cautioni

The sequence U01827 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA No translation available.
U00096 Genomic DNA Translation: AAC75581.1
AP009048 Genomic DNA Translation: BAA16422.1
PIRiG65029
RefSeqiNP_417023.1, NC_000913.3
WP_000028953.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75581; AAC75581; b2528
BAA16422; BAA16422; BAA16422
GeneIDi946999
KEGGiecj:JW2512
eco:b2528
PATRICifig|1411691.4.peg.4206

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA No translation available.
U00096 Genomic DNA Translation: AAC75581.1
AP009048 Genomic DNA Translation: BAA16422.1
PIRiG65029
RefSeqiNP_417023.1, NC_000913.3
WP_000028953.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R94X-ray2.30A/B1-105[»]
1R95X-ray2.65A/B1-105[»]
1S98X-ray2.30A/B1-107[»]
ProteinModelPortaliP0AAC8
SMRiP0AAC8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260603, 33 interactors
ComplexPortaliCPX-2132 IscA complex
DIPiDIP-35854N
IntActiP0AAC8, 5 interactors
STRINGi316385.ECDH10B_2695

Proteomic databases

EPDiP0AAC8
PaxDbiP0AAC8
PRIDEiP0AAC8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75581; AAC75581; b2528
BAA16422; BAA16422; BAA16422
GeneIDi946999
KEGGiecj:JW2512
eco:b2528
PATRICifig|1411691.4.peg.4206

Organism-specific databases

EchoBASEiEB2053
EcoGeneiEG12132 iscA

Phylogenomic databases

eggNOGiENOG4108Z4V Bacteria
COG0316 LUCA
HOGENOMiHOG000228314
InParanoidiP0AAC8
KOiK13628
PhylomeDBiP0AAC8

Enzyme and pathway databases

BioCyciEcoCyc:EG12132-MONOMER
MetaCyc:EG12132-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AAC8
PROiPR:P0AAC8

Family and domain databases

Gene3Di2.60.300.12, 1 hit
HAMAPiMF_01429 Fe_S_insert_IscA, 1 hit
InterProiView protein in InterPro
IPR000361 FeS_biogenesis
IPR016092 FeS_cluster_insertion
IPR017870 FeS_cluster_insertion_CS
IPR035903 HesB-like_dom_sf
IPR011302 IscA_proteobacteria
PfamiView protein in Pfam
PF01521 Fe-S_biosyn, 1 hit
SUPFAMiSSF89360 SSF89360, 1 hit
TIGRFAMsiTIGR02011 IscA, 1 hit
TIGR00049 TIGR00049, 1 hit
PROSITEiView protein in PROSITE
PS01152 HESB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISCA_ECOLI
AccessioniPrimary (citable) accession number: P0AAC8
Secondary accession number(s): P36539, P77691
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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