UniProtKB - P0AAB4 (UBID_ECOLI)
Protein
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
Gene
ubiD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol, an intermediate step in ubiquinone biosynthesis.UniRule annotation1 Publication
Catalytic activityi
- a 4-hydroxy-3-all-trans-polyprenylbenzoate + H+ = a 2-all-trans-polyprenylphenol + CO2UniRule annotation1 PublicationEC:4.1.1.98UniRule annotation1 Publication
Cofactori
Protein has several cofactor binding sites:- prenyl-FMNUniRule annotation1 PublicationNote: Binds 1 prenylated FMN (prenyl-FMN) per subunit.UniRule annotation1 Publication
- Mn2+UniRule annotation2 Publications
Activity regulationi
Requires phospholipid, a metal ion, dithiothreitol and at least one other so far unidentified soluble cofactor for maximal activity. Inhibited by EDTA.1 Publication
: ubiquinone biosynthesis Pathwayi
This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 175 | ManganeseUniRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 241 | ManganeseUniRule annotationCombined sources1 Publication | 1 | |
Active sitei | 290 | Proton acceptorUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity Source: EcoCyc
- carboxy-lyase activity Source: GO_Central
- identical protein binding Source: EcoCyc
- manganese ion binding Source: EcoCyc
GO - Biological processi
- protein hexamerization Source: EcoCyc
- ubiquinone biosynthetic process Source: EcoCyc
- ubiquinone biosynthetic process from chorismate Source: EcoCyc
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Ubiquinone biosynthesis |
Ligand | Flavoprotein, FMN, Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11396-MONOMER MetaCyc:EG11396-MONOMER |
UniPathwayi | UPA00232 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-octaprenyl-4-hydroxybenzoate carboxy-lyase1 PublicationUniRule annotation (EC:4.1.1.98UniRule annotation1 Publication)Alternative name(s): Polyprenyl p-hydroxybenzoate decarboxylaseUniRule annotation |
Gene namesi | Name:ubiDUniRule annotation Synonyms:yigC, yigY Ordered Locus Names:b3843, JW3819 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation1 Publication
GO - Cellular componenti
- cytoplasm Source: GO_Central
- cytosol Source: EcoCyc
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000157357 | 1 – 497 | 3-octaprenyl-4-hydroxybenzoate carboxy-lyaseAdd BLAST | 497 |
Proteomic databases
jPOSTi | P0AAB4 |
PaxDbi | P0AAB4 |
PRIDEi | P0AAB4 |
Interactioni
Subunit structurei
Homohexamer.
UniRule annotation2 Publications1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261466, 338 interactors 852623, 1 interactor |
DIPi | DIP-48249N |
IntActi | P0AAB4, 4 interactors |
STRINGi | 511145.b3843 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AAB4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AAB4 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 178 – 180 | Prenyl-FMN bindingUniRule annotationCombined sources1 Publication | 3 | |
Regioni | 192 – 194 | Prenyl-FMN bindingUniRule annotationCombined sources1 Publication | 3 | |
Regioni | 197 – 198 | Prenyl-FMN bindingUniRule annotationCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the UbiD family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0043, Bacteria |
HOGENOMi | CLU_023348_4_1_6 |
InParanoidi | P0AAB4 |
PhylomeDBi | P0AAB4 |
Family and domain databases
HAMAPi | MF_01636, UbiD, 1 hit |
InterProi | View protein in InterPro IPR002830, UbiD IPR023677, UbiD_bacteria |
PANTHERi | PTHR30108, PTHR30108, 1 hit |
Pfami | View protein in Pfam PF01977, UbiD, 1 hit |
TIGRFAMsi | TIGR00148, TIGR00148, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AAB4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDAMKYNDLR DFLTLLEQQG ELKRITLPVD PHLEITEIAD RTLRAGGPAL
60 70 80 90 100
LFENPKGYSM PVLCNLFGTP KRVAMGMGQE DVSALREVGK LLAFLKEPEP
110 120 130 140 150
PKGFRDLFDK LPQFKQVLNM PTKRLRGAPC QQKIVSGDDV DLNRIPIMTC
160 170 180 190 200
WPEDAAPLIT WGLTVTRGPH KERQNLGIYR QQLIGKNKLI MRWLSHRGGA
210 220 230 240 250
LDYQEWCAAH PGERFPVSVA LGADPATILG AVTPVPDTLS EYAFAGLLRG
260 270 280 290 300
TKTEVVKCIS NDLEVPASAE IVLEGYIEQG ETAPEGPYGD HTGYYNEVDS
310 320 330 340 350
FPVFTVTHIT QREDAIYHST YTGRPPDEPA VLGVALNEVF VPILQKQFPE
360 370 380 390 400
IVDFYLPPEG CSYRLAVVTI KKQYAGHAKR VMMGVWSFLR QFMYTKFVIV
410 420 430 440 450
CDDDVNARDW NDVIWAITTR MDPARDTVLV ENTPIDYLDF ASPVSGLGSK
460 470 480 490
MGLDATNKWP GETQREWGRP IKKDPDVVAH IDAIWDELAI FNNGKSA
Sequence cautioni
The sequence AAA67639 differs from that shown. Reason: Frameshift.Curated
The sequence CAA46146 differs from that shown. Reason: Frameshift.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 452 | G → R in AN66; inactive protein. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65013 Genomic DNA Translation: CAA46146.1 Frameshift. M87049 Genomic DNA Translation: AAA67639.1 Frameshift. M87049 Genomic DNA Translation: AAA67640.1 U00096 Genomic DNA Translation: AAC76846.1 AP009048 Genomic DNA Translation: BAE77460.1 |
PIRi | D65189 S20905 S30734 |
RefSeqi | NP_418285.1, NC_000913.3 WP_000339804.1, NZ_STEB01000021.1 |
Genome annotation databases
EnsemblBacteriai | AAC76846; AAC76846; b3843 BAE77460; BAE77460; BAE77460 |
GeneIDi | 49586873 948326 |
KEGGi | ecj:JW3819 eco:b3843 |
PATRICi | fig|1411691.4.peg.2867 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65013 Genomic DNA Translation: CAA46146.1 Frameshift. M87049 Genomic DNA Translation: AAA67639.1 Frameshift. M87049 Genomic DNA Translation: AAA67640.1 U00096 Genomic DNA Translation: AAC76846.1 AP009048 Genomic DNA Translation: BAE77460.1 |
PIRi | D65189 S20905 S30734 |
RefSeqi | NP_418285.1, NC_000913.3 WP_000339804.1, NZ_STEB01000021.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IDB | X-ray | 2.90 | A/B/C | 1-497 | [»] | |
5M1B | X-ray | 3.15 | A/B/C | 1-497 | [»] | |
5M1C | X-ray | 2.75 | A/B/C | 1-497 | [»] | |
5M1D | X-ray | 2.70 | A/B/C | 1-497 | [»] | |
5M1E | X-ray | 2.62 | A/B/C | 1-497 | [»] | |
SMRi | P0AAB4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261466, 338 interactors 852623, 1 interactor |
DIPi | DIP-48249N |
IntActi | P0AAB4, 4 interactors |
STRINGi | 511145.b3843 |
Proteomic databases
jPOSTi | P0AAB4 |
PaxDbi | P0AAB4 |
PRIDEi | P0AAB4 |
Genome annotation databases
EnsemblBacteriai | AAC76846; AAC76846; b3843 BAE77460; BAE77460; BAE77460 |
GeneIDi | 49586873 948326 |
KEGGi | ecj:JW3819 eco:b3843 |
PATRICi | fig|1411691.4.peg.2867 |
Organism-specific databases
EchoBASEi | EB1369 |
Phylogenomic databases
eggNOGi | COG0043, Bacteria |
HOGENOMi | CLU_023348_4_1_6 |
InParanoidi | P0AAB4 |
PhylomeDBi | P0AAB4 |
Enzyme and pathway databases
UniPathwayi | UPA00232 |
BioCyci | EcoCyc:EG11396-MONOMER MetaCyc:EG11396-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AAB4 |
PROi | PR:P0AAB4 |
Family and domain databases
HAMAPi | MF_01636, UbiD, 1 hit |
InterProi | View protein in InterPro IPR002830, UbiD IPR023677, UbiD_bacteria |
PANTHERi | PTHR30108, PTHR30108, 1 hit |
Pfami | View protein in Pfam PF01977, UbiD, 1 hit |
TIGRFAMsi | TIGR00148, TIGR00148, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBID_ECOLI | |
Accessioni | P0AAB4Primary (citable) accession number: P0AAB4 Secondary accession number(s): P26615 Q47714 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | September 13, 2005 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families