UniProtKB - P0AA86 (DSBE_ECOLI)
Protein
Thiol:disulfide interchange protein DsbE
Gene
dsbE
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme lyase. DsbE is maintained in a reduced state by DsbD.
GO - Molecular functioni
- disulfide oxidoreductase activity Source: EcoCyc
GO - Biological processi
- cytochrome complex assembly Source: EcoCyc
- oxidation-reduction process Source: EcoCyc
Keywordsi
Biological process | Cytochrome c-type biogenesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12053-MONOMER MetaCyc:EG12053-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Thiol:disulfide interchange protein DsbEAlternative name(s): Cytochrome c biogenesis protein CcmG |
Gene namesi | Name:dsbE Synonyms:ccmG, yejQ Ordered Locus Names:b2195, JW2183 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 5 – 25 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 26 – 185 | PeriplasmicSequence analysisAdd BLAST | 160 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- intrinsic component of periplasmic side of plasma membrane Source: EcoCyc
- outer membrane-bounded periplasmic space Source: InterPro
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 80 | C → S: Drastic decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 83 | C → S: Drastic decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201293 | 1 – 185 | Thiol:disulfide interchange protein DsbEAdd BLAST | 185 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 80 ↔ 83 | Redox-activeCurated |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P0AA86 |
PaxDbi | P0AA86 |
PRIDEi | P0AA86 |
Interactioni
Binary interactionsi
P0AA86
With | #Exp. | IntAct |
---|---|---|
dsbD [P36655] | 4 | EBI-9014059,EBI-9014057 |
Protein-protein interaction databases
BioGRIDi | 4263405, 34 interactors |
DIPi | DIP-48446N |
IntActi | P0AA86, 2 interactors |
STRINGi | 511145.b2195 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AA86 |
SMRi | P0AA86 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AA86 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 177 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 139 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0526, Bacteria |
HOGENOMi | CLU_042529_19_1_6 |
InParanoidi | P0AA86 |
PhylomeDBi | P0AA86 |
Family and domain databases
CDDi | cd03010, TlpA_like_DsbE, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR004799, Periplasmic_diS_OxRdtase_DsbE IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00385, dsbE, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AA86-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRKVLLIPL IIFLAIAAAL LWQLARNAEG DDPTNLESAL IGKPVPKFRL
60 70 80 90 100
ESLDNPGQFY QADVLTQGKP VLLNVWATWC PTCRAEHQYL NQLSAQGIRV
110 120 130 140 150
VGMNYKDDRQ KAISWLKELG NPYALSLFDG DGMLGLDLGV YGAPETFLID
160 170 180
GNGIIRYRHA GDLNPRVWEE EIKPLWEKYS KEAAQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16387.1 U00096 Genomic DNA Translation: AAC75255.1 AP009048 Genomic DNA Translation: BAE76658.1 |
PIRi | A64989 |
RefSeqi | NP_416699.1, NC_000913.3 WP_000824439.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75255; AAC75255; b2195 BAE76658; BAE76658; BAE76658 |
GeneIDi | 49583986 949073 |
KEGGi | ecj:JW2183 eco:b2195 |
PATRICi | fig|511145.12.peg.2284 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16387.1 U00096 Genomic DNA Translation: AAC75255.1 AP009048 Genomic DNA Translation: BAE76658.1 |
PIRi | A64989 |
RefSeqi | NP_416699.1, NC_000913.3 WP_000824439.1, NZ_STEB01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Z5Y | X-ray | 1.94 | E | 43-185 | [»] | |
2B1K | X-ray | 1.90 | A | 19-185 | [»] | |
2B1L | X-ray | 1.90 | A/B | 58-185 | [»] | |
2G0F | X-ray | 2.20 | A | 19-185 | [»] | |
3K8N | X-ray | 2.30 | A | 1-185 | [»] | |
BMRBi | P0AA86 | |||||
SMRi | P0AA86 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263405, 34 interactors |
DIPi | DIP-48446N |
IntActi | P0AA86, 2 interactors |
STRINGi | 511145.b2195 |
Proteomic databases
jPOSTi | P0AA86 |
PaxDbi | P0AA86 |
PRIDEi | P0AA86 |
Genome annotation databases
EnsemblBacteriai | AAC75255; AAC75255; b2195 BAE76658; BAE76658; BAE76658 |
GeneIDi | 49583986 949073 |
KEGGi | ecj:JW2183 eco:b2195 |
PATRICi | fig|511145.12.peg.2284 |
Organism-specific databases
EchoBASEi | EB1984 |
Phylogenomic databases
eggNOGi | COG0526, Bacteria |
HOGENOMi | CLU_042529_19_1_6 |
InParanoidi | P0AA86 |
PhylomeDBi | P0AA86 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12053-MONOMER MetaCyc:EG12053-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AA86 |
PROi | PR:P0AA86 |
Family and domain databases
CDDi | cd03010, TlpA_like_DsbE, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR004799, Periplasmic_diS_OxRdtase_DsbE IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00385, dsbE, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DSBE_ECOLI | |
Accessioni | P0AA86Primary (citable) accession number: P0AA86 Secondary accession number(s): P33926, Q2MAP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | September 13, 2005 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families