UniProtKB - P0AA63 (YQJA_ECOLI)
Protein
Inner membrane protein YqjA
Gene
yqjA
Organism
Escherichia coli (strain K12)
Status
Functioni
May be a membrane transporter required for proton motive force (PMF)-dependent drug efflux. Required, with YghB, for the proper export of certain periplasmic amidases and, possibly, other Tat substrates. May play a role in determining membrane lipid composition.3 Publications
GO - Molecular functioni
- transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- FtsZ-dependent cytokinesis Source: EcoCyc
- transmembrane transport Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:G7609-MONOMER |
Protein family/group databases
TCDBi | 9.B.27.2.2, the death effector domain a (deda) family |
Names & Taxonomyi
Protein namesi | Recommended name: Inner membrane protein YqjA |
Gene namesi | Name:yqjA Ordered Locus Names:b3095, JW3066 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 27 | PeriplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 28 – 48 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 49 – 52 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 74 – 94 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 95 – 154 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 155 – 175 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 176 – 191 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 192 – 212 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 213 – 220 | CytoplasmicSequence analysis | 8 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Double mutants lacking both yghB and yqjA show incomplete cell division, temperature sensitivity and altered phospholipid levels. They are also hypersensitive to several compounds known to be exported by other drug efflux proteins, including ethidium bromide, methyl viologen, acriflavine and beta-lactam antibiotics. Expression of either yghB or yqjA can restore the wild-type phenotype, suggesting that these proteins have redundant functions. Both individual null mutant strains grow normally at all temperatures.3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 39 | E → A or Q: Abolishes the ability to restore growth, cell division or drug resistance in double mutant. 1 Publication | 1 | |
Mutagenesisi | 51 | D → A or N: Abolishes the ability to restore growth, cell division or drug resistance in double mutant. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161417 | 1 – 220 | Inner membrane protein YqjAAdd BLAST | 220 |
Proteomic databases
PaxDbi | P0AA63 |
PRIDEi | P0AA63 |
Expressioni
Inductioni
Regulated by the CpxA/CpxR two-component system.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262411, 70 interactors |
STRINGi | 511145.b3095 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DedA family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0586, Bacteria |
HOGENOMi | CLU_044208_6_2_6 |
InParanoidi | P0AA63 |
PhylomeDBi | P0AA63 |
Family and domain databases
InterProi | View protein in InterPro IPR032818, DedA IPR032816, SNARE_assoc |
PANTHERi | PTHR30353, PTHR30353, 1 hit |
Pfami | View protein in Pfam PF09335, SNARE_assoc, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AA63-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELLTQLLQA LWAQDFETLA NPSMIGMLYF VLFVILFLEN GLLPAAFLPG
60 70 80 90 100
DSLLVLVGVL IAKGAMGYPQ TILLLTVAAS LGCWVSYIQG RWLGNTRTVQ
110 120 130 140 150
NWLSHLPAHY HQRAHHLFHK HGLSALLIGR FIAFVRTLLP TIAGLSGLNN
160 170 180 190 200
ARFQFFNWMS GLLWVLILTT LGYMLGKTPV FLKYEDQLMS CLMLLPVVLL
210 220
VFGLAGSLVV LWKKKYGNRG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57899.1 U00096 Genomic DNA Translation: AAC76130.1 AP009048 Genomic DNA Translation: BAE77145.1 D13328 Genomic DNA No translation available. |
PIRi | D65098 |
RefSeqi | NP_417566.1, NC_000913.3 WP_000422149.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76130; AAC76130; b3095 BAE77145; BAE77145; BAE77145 |
GeneIDi | 58461268 947643 |
KEGGi | ecj:JW3066 eco:b3095 |
PATRICi | fig|1411691.4.peg.3633 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57899.1 U00096 Genomic DNA Translation: AAC76130.1 AP009048 Genomic DNA Translation: BAE77145.1 D13328 Genomic DNA No translation available. |
PIRi | D65098 |
RefSeqi | NP_417566.1, NC_000913.3 WP_000422149.1, NZ_STEB01000001.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262411, 70 interactors |
STRINGi | 511145.b3095 |
Protein family/group databases
TCDBi | 9.B.27.2.2, the death effector domain a (deda) family |
Proteomic databases
PaxDbi | P0AA63 |
PRIDEi | P0AA63 |
Genome annotation databases
EnsemblBacteriai | AAC76130; AAC76130; b3095 BAE77145; BAE77145; BAE77145 |
GeneIDi | 58461268 947643 |
KEGGi | ecj:JW3066 eco:b3095 |
PATRICi | fig|1411691.4.peg.3633 |
Organism-specific databases
EchoBASEi | EB2596 |
Phylogenomic databases
eggNOGi | COG0586, Bacteria |
HOGENOMi | CLU_044208_6_2_6 |
InParanoidi | P0AA63 |
PhylomeDBi | P0AA63 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7609-MONOMER |
Miscellaneous databases
PROi | PR:P0AA63 |
Family and domain databases
InterProi | View protein in InterPro IPR032818, DedA IPR032816, SNARE_assoc |
PANTHERi | PTHR30353, PTHR30353, 1 hit |
Pfami | View protein in Pfam PF09335, SNARE_assoc, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YQJA_ECOLI | |
Accessioni | P0AA63Primary (citable) accession number: P0AA63 Secondary accession number(s): P42614, Q2M9B1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | September 13, 2005 | |
Last modified: | February 10, 2021 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families