UniProtKB - P0AA43 (RSUA_ECOLI)
Protein
Ribosomal small subunit pseudouridine synthase A
Gene
rsuA
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA.2 Publications
Catalytic activityi
- EC:5.4.99.192 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 102 | Nucleophile1 Publication | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: GO_Central
- RNA binding Source: UniProtKB-KW
- rRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Keywordsi
Molecular function | Isomerase, RNA-binding |
Biological process | rRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12044-MONOMER MetaCyc:EG12044-MONOMER |
BRENDAi | 5.4.99.19, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal small subunit pseudouridine synthase A (EC:5.4.99.19)Alternative name(s): 16S pseudouridylate 516 synthase 16S rRNA pseudouridine(516) synthase rRNA pseudouridylate synthase A rRNA-uridine isomerase A |
Gene namesi | Name:rsuA Synonyms:yejD Ordered Locus Names:b2183, JW2171 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 102 | D → N or T: Loss of activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB03419, Uracil DB03685, Uridine monophosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000099966 | 1 – 231 | Ribosomal small subunit pseudouridine synthase AAdd BLAST | 231 |
Proteomic databases
jPOSTi | P0AA43 |
PaxDbi | P0AA43 |
PRIDEi | P0AA43 |
2D gel databases
SWISS-2DPAGEi | P0AA43 |
Interactioni
Subunit structurei
Monomer.
Binary interactionsi
P0AA43
With | #Exp. | IntAct |
---|---|---|
rpsJ [P0A7R5] | 3 | EBI-557810,EBI-544602 |
Protein-protein interaction databases
BioGRIDi | 4260474, 48 interactors |
DIPi | DIP-35902N |
IntActi | P0AA43, 40 interactors |
STRINGi | 511145.b2183 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AA43 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AA43 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 68 | S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST | 68 |
Sequence similaritiesi
Belongs to the pseudouridine synthase RsuA family.Curated
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_1_2_6 |
InParanoidi | P0AA43 |
PhylomeDBi | P0AA43 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit 3.30.70.1560, 1 hit |
InterProi | View protein in InterPro IPR042092, PsdUridine_s_RsuA/RluB/E/F_cat IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AA43-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GEIVRNAAFK LLPEHDVAYD
60 70 80 90 100
GNPLAQQHGP RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAWKLHAAGR
110 120 130 140 150
LDIDTTGLVL MTDDGQWSHR ITSPRHHCEK TYLVTLESPV ADDTAEQFAK
160 170 180 190 200
GVQLHNEKDL TKPAVLEVIT PTQVRLTISE GRYHQVKRMF AAVGNHVVEL
210 220 230
HRERIGGITL DADLAPGEYR PLTEEEIASV V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16377.1 U00096 Genomic DNA Translation: AAC75244.1 AP009048 Genomic DNA Translation: BAE76648.1 |
PIRi | F64987 |
RefSeqi | NP_416688.1, NC_000913.3 WP_001234850.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75244; AAC75244; b2183 BAE76648; BAE76648; BAE76648 |
GeneIDi | 58462714 945378 |
KEGGi | ecj:JW2171 eco:b2183 |
PATRICi | fig|1411691.4.peg.53 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16377.1 U00096 Genomic DNA Translation: AAC75244.1 AP009048 Genomic DNA Translation: BAE76648.1 |
PIRi | F64987 |
RefSeqi | NP_416688.1, NC_000913.3 WP_001234850.1, NZ_STEB01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KSK | X-ray | 2.00 | A | 1-231 | [»] | |
1KSL | X-ray | 2.10 | A | 1-231 | [»] | |
1KSV | X-ray | 2.65 | A | 1-231 | [»] | |
SMRi | P0AA43 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260474, 48 interactors |
DIPi | DIP-35902N |
IntActi | P0AA43, 40 interactors |
STRINGi | 511145.b2183 |
Chemistry databases
DrugBanki | DB03419, Uracil DB03685, Uridine monophosphate |
2D gel databases
SWISS-2DPAGEi | P0AA43 |
Proteomic databases
jPOSTi | P0AA43 |
PaxDbi | P0AA43 |
PRIDEi | P0AA43 |
Genome annotation databases
EnsemblBacteriai | AAC75244; AAC75244; b2183 BAE76648; BAE76648; BAE76648 |
GeneIDi | 58462714 945378 |
KEGGi | ecj:JW2171 eco:b2183 |
PATRICi | fig|1411691.4.peg.53 |
Organism-specific databases
EchoBASEi | EB1978 |
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_1_2_6 |
InParanoidi | P0AA43 |
PhylomeDBi | P0AA43 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12044-MONOMER MetaCyc:EG12044-MONOMER |
BRENDAi | 5.4.99.19, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AA43 |
PROi | PR:P0AA43 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit 3.30.70.1560, 1 hit |
InterProi | View protein in InterPro IPR042092, PsdUridine_s_RsuA/RluB/E/F_cat IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit PS50889, S4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSUA_ECOLI | |
Accessioni | P0AA43Primary (citable) accession number: P0AA43 Secondary accession number(s): P33918, Q2MAQ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | September 13, 2005 | |
Last modified: | February 10, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families