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UniProtKB - P0AA37 (RLUA_ECOLI)
Protein
Dual-specificity RNA pseudouridine synthase RluA
Gene
rluA
Organism
Escherichia coli (strain K12)
Status
Functioni
Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs.
2 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 64 | 1 Publication | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: EcoCyc
- RNA binding Source: InterPro
- rRNA pseudouridine synthase activity Source: EcoCyc
- tRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
- pseudouridine synthesis Source: EcoliWiki
- RNA modification Source: EcoliWiki
- rRNA processing Source: EcoliWiki
- rRNA pseudouridine synthesis Source: EcoliWiki
- tRNA processing Source: EcoliWiki
- tRNA pseudouridine synthesis Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | rRNA processing, tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12609-MONOMER |
BRENDAi | 5.4.99.28, 2026 5.4.99.29, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Dual-specificity RNA pseudouridine synthase RluACurated (EC:5.4.99.281 Publication, EC:5.4.99.291 Publication)Alternative name(s): 23S rRNA pseudouridine(746) synthaseCurated Ribosomal large subunit pseudouridine synthase ACurated rRNA pseudouridylate synthase ACurated rRNA-uridine isomerase ACurated tRNA pseudouridine(32) synthaseCurated |
Gene namesi | Name:rluA Synonyms:yabO Ordered Locus Names:b0058, JW0057 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 28 | K → M or R: Forms inclusion bodies, indicating precipitation even within the cell. 1 Publication | 1 | |
Mutagenesisi | 29 | P → G or L: Reduced structural stability and decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 64 | D → N or T: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 117 | C → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 128 | C → A: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000162651 | 2 – 219 | Dual-specificity RNA pseudouridine synthase RluAAdd BLAST | 218 |
Proteomic databases
jPOSTi | P0AA37 |
PaxDbi | P0AA37 |
PRIDEi | P0AA37 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262040, 44 interactors 850621, 3 interactors |
DIPi | DIP-47988N |
IntActi | P0AA37, 4 interactors |
STRINGi | 511145.b0058 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0AA37 |
SMRi | P0AA37 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AA37 |
Family & Domainsi
Sequence similaritiesi
Belongs to the pseudouridine synthase RluA family.Curated
Phylogenomic databases
eggNOGi | COG0564, Bacteria |
HOGENOMi | CLU_016902_11_1_6 |
InParanoidi | P0AA37 |
OMAi | HVSQSRM |
PhylomeDBi | P0AA37 |
Family and domain databases
CDDi | cd02869, PseudoU_synth_RluA_like, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR006225, PsdUridine_synth_RluC/D IPR006224, PsdUridine_synth_RluC/D_CS IPR006145, PsdUridine_synth_RsuA/RluA |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00005, rluA_subfam, 1 hit |
PROSITEi | View protein in PROSITE PS01129, PSI_RLU, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AA37-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI
60 70 80 90 100
QRDYPQAESV HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV
110 120 130 140 150
WGHPSPAEGL VDLPLICDWP NRPKQKVCYE TGKPAQTEYE VVEYAADNTA
160 170 180 190 200
RVVLKPITGR SHQLRVHMLA LGHPILGDRF YASPEARAMA PRLLLHAEML
210
TITHPAYGNS MTFKAPADF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73169.1 AP009048 Genomic DNA Translation: BAE76039.1 |
PIRi | B64727 |
RefSeqi | NP_414600.1, NC_000913.3 WP_000525176.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73169; AAC73169; b0058 BAE76039; BAE76039; BAE76039 |
GeneIDi | 66671653 946262 |
KEGGi | ecj:JW0057 eco:b0058 |
PATRICi | fig|1411691.4.peg.2225 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73169.1 AP009048 Genomic DNA Translation: BAE76039.1 |
PIRi | B64727 |
RefSeqi | NP_414600.1, NC_000913.3 WP_000525176.1, NZ_STEB01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2I82 | X-ray | 2.05 | A/B/C/D | 3-219 | [»] | |
AlphaFoldDBi | P0AA37 | |||||
SMRi | P0AA37 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262040, 44 interactors 850621, 3 interactors |
DIPi | DIP-47988N |
IntActi | P0AA37, 4 interactors |
STRINGi | 511145.b0058 |
Proteomic databases
jPOSTi | P0AA37 |
PaxDbi | P0AA37 |
PRIDEi | P0AA37 |
Genome annotation databases
EnsemblBacteriai | AAC73169; AAC73169; b0058 BAE76039; BAE76039; BAE76039 |
GeneIDi | 66671653 946262 |
KEGGi | ecj:JW0057 eco:b0058 |
PATRICi | fig|1411691.4.peg.2225 |
Organism-specific databases
EchoBASEi | EB2493 |
Phylogenomic databases
eggNOGi | COG0564, Bacteria |
HOGENOMi | CLU_016902_11_1_6 |
InParanoidi | P0AA37 |
OMAi | HVSQSRM |
PhylomeDBi | P0AA37 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12609-MONOMER |
BRENDAi | 5.4.99.28, 2026 5.4.99.29, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AA37 |
PROi | PR:P0AA37 |
Family and domain databases
CDDi | cd02869, PseudoU_synth_RluA_like, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR006225, PsdUridine_synth_RluC/D IPR006224, PsdUridine_synth_RluC/D_CS IPR006145, PsdUridine_synth_RsuA/RluA |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00005, rluA_subfam, 1 hit |
PROSITEi | View protein in PROSITE PS01129, PSI_RLU, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RLUA_ECOLI | |
Accessioni | P0AA37Primary (citable) accession number: P0AA37 Secondary accession number(s): P39219, Q2MCG7, Q83MG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families