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UniProtKB - P0AA25 (THIO_ECOLI)
Protein
Thioredoxin 1
Gene
trxA
Organism
Escherichia coli (strain K12)
Status
Functioni
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 27 | Deprotonates C-terminal active site Cys | 1 | |
Active sitei | 33 | Nucleophile | 1 | |
Sitei | 34 | Contributes to redox potential value | 1 | |
Sitei | 35 | Contributes to redox potential value | 1 | |
Active sitei | 36 | Nucleophile | 1 |
GO - Molecular functioni
- DNA polymerase processivity factor activity Source: FlyBase
- oxidoreductase activity Source: GO_Central
- protein-disulfide reductase activity Source: EcoCyc
GO - Biological processi
- cell redox homeostasis Source: EcoCyc
- glycerol ether metabolic process Source: InterPro
- positive regulation of DNA-directed DNA polymerase activity Source: FlyBase
Keywordsi
Biological process | Electron transport, Host-virus interaction, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:RED-THIOREDOXIN-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Thioredoxin 1Short name: Trx-1 |
Gene namesi | Name:trxA Synonyms:fipA, tsnC Ordered Locus Names:b3781, JW5856 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000120096 | 2 – 109 | Thioredoxin 1Add BLAST | 108 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 33 ↔ 36 | Redox-activePROSITE-ProRule annotation1 Publication | ||
Modified residuei | 70 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
Acetylation, Disulfide bondProteomic databases
jPOSTi | P0AA25 |
PaxDbi | P0AA25 |
PRIDEi | P0AA25 |
2D gel databases
SWISS-2DPAGEi | P0AA25 |
PTM databases
CarbonylDBi | P0AA25 |
iPTMneti | P0AA25 |
Interactioni
Subunit structurei
Monomer.
Interacts with bacteriophage T3 DNA polymerase.
1 PublicationBinary interactionsi
P0AA25
Protein-protein interaction databases
BioGRIDi | 4263316, 300 interactors 852588, 8 interactors |
DIPi | DIP-31856N |
IntActi | P0AA25, 99 interactors |
MINTi | P0AA25 |
STRINGi | 511145.b3781 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AA25 |
SASBDBi | P0AA25 |
SMRi | P0AA25 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AA25 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 109 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 108 |
Sequence similaritiesi
Belongs to the thioredoxin family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG3118, Bacteria |
HOGENOMi | CLU_090389_10_2_6 |
InParanoidi | P0AA25 |
PhylomeDBi | P0AA25 |
Family and domain databases
InterProi | View protein in InterPro IPR005746, Thioredoxin IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 1 hit |
PIRSFi | PIRSF000077, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR01068, thioredoxin, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AA25-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY
60 70 80 90 100
QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL
KEFLDANLA
Sequence cautioni
The sequence AAA24534 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAA67582 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 – 73 | GI → IG AA sequence (PubMed:4883076).Curated | 2 | |
Sequence conflicti | 88 | A → AS in AAA24696 (PubMed:1379743).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26133 Genomic DNA Translation: AAA24693.1 K02845 Genomic DNA Translation: AAA24534.1 Different initiation. M10424 Genomic DNA Translation: AAA24533.1 M54881 Genomic DNA Translation: AAA24696.1 M12779 Genomic DNA Translation: AAA24694.1 M87049 Genomic DNA Translation: AAA67582.1 Different initiation. U00096 Genomic DNA Translation: AAC76786.2 AP009048 Genomic DNA Translation: BAE77517.1 |
PIRi | A91519, TXEC |
RefSeqi | NP_418228.2, NC_000913.3 WP_001280776.1, NZ_STEB01000021.1 |
Genome annotation databases
EnsemblBacteriai | AAC76786; AAC76786; b3781 BAE77517; BAE77517; BAE77517 |
GeneIDi | 64173577 67514182 948289 |
KEGGi | ecj:JW5856 eco:b3781 |
PATRICi | fig|511145.12.peg.3896 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M26133 Genomic DNA Translation: AAA24693.1 K02845 Genomic DNA Translation: AAA24534.1 Different initiation. M10424 Genomic DNA Translation: AAA24533.1 M54881 Genomic DNA Translation: AAA24696.1 M12779 Genomic DNA Translation: AAA24694.1 M87049 Genomic DNA Translation: AAA67582.1 Different initiation. U00096 Genomic DNA Translation: AAC76786.2 AP009048 Genomic DNA Translation: BAE77517.1 |
PIRi | A91519, TXEC |
RefSeqi | NP_418228.2, NC_000913.3 WP_001280776.1, NZ_STEB01000021.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F6M | X-ray | 2.95 | C/D/G/H | 2-109 | [»] | |
1KEB | X-ray | 1.80 | A/B | 2-109 | [»] | |
1M7T | NMR | - | A | 32-108 | [»] | |
1OAZ | X-ray | 2.78 | A/B | 2-109 | [»] | |
1SKR | X-ray | 2.40 | B | 2-109 | [»] | |
1SKS | X-ray | 2.30 | B | 2-109 | [»] | |
1SKW | X-ray | 2.30 | B | 2-109 | [»] | |
1SL0 | X-ray | 3.20 | B/D | 2-109 | [»] | |
1SL1 | X-ray | 2.20 | B | 2-109 | [»] | |
1SL2 | X-ray | 2.30 | B | 2-109 | [»] | |
1SRX | X-ray | 2.80 | A | 2-109 | [»] | |
1T7P | X-ray | 2.20 | B | 2-109 | [»] | |
1T8E | X-ray | 2.54 | B | 2-109 | [»] | |
1THO | X-ray | 2.30 | A | 2-109 | [»] | |
1TK0 | X-ray | 2.30 | B | 2-109 | [»] | |
1TK5 | X-ray | 2.20 | B | 2-109 | [»] | |
1TK8 | X-ray | 2.50 | B | 2-109 | [»] | |
1TKD | X-ray | 2.49 | B | 2-109 | [»] | |
1TXX | X-ray | 2.20 | A | 2-109 | [»] | |
1X9M | X-ray | 2.10 | B | 2-109 | [»] | |
1X9S | X-ray | 2.70 | B | 2-109 | [»] | |
1X9W | X-ray | 2.30 | B | 2-109 | [»] | |
1XOA | NMR | - | A | 2-109 | [»] | |
1XOB | NMR | - | A | 2-109 | [»] | |
1ZCP | X-ray | 2.30 | A/B/C/D | 2-109 | [»] | |
1ZYQ | X-ray | 2.70 | B | 2-109 | [»] | |
1ZZY | X-ray | 2.50 | A/B | 2-109 | [»] | |
2AJQ | X-ray | 2.60 | B/I | 2-109 | [»] | |
2BTO | X-ray | 2.50 | T | 2-109 | [»] | |
2EIO | X-ray | 2.60 | A/B/C/D | 2-109 | [»] | |
2EIQ | X-ray | 1.90 | A/B | 2-109 | [»] | |
2EIR | X-ray | 2.50 | A/B/C/D | 2-109 | [»] | |
2FCH | X-ray | 2.60 | A/B/C/D/E/F/G | 2-109 | [»] | |
2FD3 | X-ray | 2.45 | A/B | 2-109 | [»] | |
2H6X | X-ray | 2.60 | A/B | 2-109 | [»] | |
2H6Y | X-ray | 2.40 | A/B | 2-109 | [»] | |
2H6Z | X-ray | 2.25 | A/B | 2-109 | [»] | |
2H70 | X-ray | 2.70 | A/B | 2-109 | [»] | |
2H71 | X-ray | 2.20 | A/B | 2-109 | [»] | |
2H72 | X-ray | 2.25 | A/B | 2-109 | [»] | |
2H73 | X-ray | 2.45 | A/B | 2-109 | [»] | |
2H74 | X-ray | 2.40 | A/B | 4-109 | [»] | |
2H75 | X-ray | 2.20 | A/B | 2-109 | [»] | |
2H76 | X-ray | 2.25 | A/B | 2-109 | [»] | |
2O8V | X-ray | 3.00 | B | 2-109 | [»] | |
2TIR | X-ray | 2.00 | A | 2-109 | [»] | |
2TRX | X-ray | 1.68 | A/B | 2-109 | [»] | |
3DYR | X-ray | 2.00 | A/B | 2-109 | [»] | |
4HU7 | X-ray | 1.40 | A/B | 2-109 | [»] | |
4HU9 | X-ray | 1.55 | A | 2-109 | [»] | |
4HUA | X-ray | 1.10 | A | 2-109 | [»] | |
4X43 | X-ray | 1.65 | A/B/C | 2-109 | [»] | |
5HR0 | X-ray | 1.31 | A/B | 1-109 | [»] | |
5HR1 | X-ray | 2.14 | A/B/C/D/E/F/G | 1-107 | [»] | |
5HR2 | X-ray | 1.20 | A | 1-109 | [»] | |
5HR3 | X-ray | 1.10 | A/B | 1-109 | [»] | |
5XOC | X-ray | 2.40 | B | 2-109 | [»] | |
6GD1 | X-ray | 2.01 | A/B | 1-109 | [»] | |
6GDG | electron microscopy | 4.11 | A | 2-109 | [»] | |
6H7J | X-ray | 2.80 | E/F | 2-109 | [»] | |
6H7L | X-ray | 2.70 | E/F | 2-109 | [»] | |
6H7M | X-ray | 2.76 | E/F | 2-109 | [»] | |
6H7N | X-ray | 2.50 | E/F | 2-109 | [»] | |
6H7O | X-ray | 2.80 | E/F | 2-109 | [»] | |
6IBL | X-ray | 2.70 | A/B | 2-109 | [»] | |
6LUR | X-ray | 2.00 | A/B/C/D/E/F/G/H | 1-109 | [»] | |
6N7W | electron microscopy | 4.50 | I | 1-109 | [»] | |
6P7E | X-ray | 3.00 | E/F/G/H | 1-109 | [»] | |
6Y4Y | X-ray | 1.75 | A/B/C/D | 1-109 | [»] | |
6Y4Z | X-ray | 1.90 | A/B/C/D | 1-109 | [»] | |
6YEV | X-ray | 2.94 | E/F/G | 1-109 | [»] | |
BMRBi | P0AA25 | |||||
SASBDBi | P0AA25 | |||||
SMRi | P0AA25 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263316, 300 interactors 852588, 8 interactors |
DIPi | DIP-31856N |
IntActi | P0AA25, 99 interactors |
MINTi | P0AA25 |
STRINGi | 511145.b3781 |
PTM databases
CarbonylDBi | P0AA25 |
iPTMneti | P0AA25 |
2D gel databases
SWISS-2DPAGEi | P0AA25 |
Proteomic databases
jPOSTi | P0AA25 |
PaxDbi | P0AA25 |
PRIDEi | P0AA25 |
Genome annotation databases
EnsemblBacteriai | AAC76786; AAC76786; b3781 BAE77517; BAE77517; BAE77517 |
GeneIDi | 64173577 67514182 948289 |
KEGGi | ecj:JW5856 eco:b3781 |
PATRICi | fig|511145.12.peg.3896 |
Organism-specific databases
EchoBASEi | EB1024 |
Phylogenomic databases
eggNOGi | COG3118, Bacteria |
HOGENOMi | CLU_090389_10_2_6 |
InParanoidi | P0AA25 |
PhylomeDBi | P0AA25 |
Enzyme and pathway databases
BioCyci | EcoCyc:RED-THIOREDOXIN-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AA25 |
PROi | PR:P0AA25 |
Family and domain databases
InterProi | View protein in InterPro IPR005746, Thioredoxin IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 1 hit |
PIRSFi | PIRSF000077, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR01068, thioredoxin, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | THIO_ECOLI | |
Accessioni | P0AA25Primary (citable) accession number: P0AA25 Secondary accession number(s): P00274 Q8XAT2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families