UniProtKB - P0A9X4 (MREB_ECOLI)
Cell shape-determining protein MreB
mreB
Functioni
Forms membrane-associated dynamic filaments that are essential for cell shape determination (PubMed:15612918, PubMed:21903929).
Acts by regulating cell wall synthesis and cell elongation, and thus cell shape (PubMed:21903929).
A feedback loop between cell geometry and MreB localization maintains elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature (PubMed:24550515).
Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on MreB polymerization (PubMed:21903929).
Rotation may contribute to the robust maintenance of rod shape (PubMed:21903929).
3 PublicationsActivity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 19 – 21 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 168 – 170 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 216 – 219 | ATPUniRule annotation | 4 | |
Nucleotide bindingi | 296 – 299 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: EcoCyc
GO - Biological processi
- cell morphogenesis Source: InterPro
- FtsZ-dependent cytokinesis Source: EcoCyc
- negative regulation of cell division Source: CACAO
- regulation of cell shape Source: EcoCyc
- regulation of chromosome segregation Source: EcoliWiki
Keywordsi
Biological process | Cell shape |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10608-MONOMER |
Protein family/group databases
TCDBi | 9.B.157.1.3, the cell shape-determining mrebcd (mrebcd) family |
Names & Taxonomyi
Protein namesi | Recommended name: Cell shape-determining protein MreBUniRule annotationCuratedAlternative name(s): Actin-like MreB proteinCurated Rod shape-determining protein MreBCurated |
Gene namesi | Name:mreB1 Publication Synonyms:envB, rodY Ordered Locus Names:b3251, JW3220 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Membrane-associated (PubMed:15612918). Preferentially localizes to regions of negative curvature (PubMed:24550515). May associate with the membrane via MreC (PubMed:15612918). Localization depends on MreC, MreD and RodA (PubMed:15612918).2 Publications
Cytoskeleton
- cytoskeleton Source: EcoCyc
- polymeric cytoskeletal fiber Source: EcoCyc
Plasma Membrane
- intrinsic component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000062759 | 1 – 347 | Cell shape-determining protein MreBAdd BLAST | 347 |
Proteomic databases
jPOSTi | P0A9X4 |
PaxDbi | P0A9X4 |
PRIDEi | P0A9X4 |
Interactioni
Subunit structurei
Forms polymers in the presence of ATP or GTP (PubMed:23235161).
Forms an essential membrane-bound complex with MreC and MreD.
Interacts directly with MreC but not with MreD (PubMed:15612918).
Interacts with FtsZ, toxins CbtA and CptA, and cytoskeleton bundling-enhancing protein CbeA (PubMed:21166897, PubMed:22239607, PubMed:22515815).
5 PublicationsBinary interactionsi
P0A9X4
With | #Exp. | IntAct |
---|---|---|
cbeA [P76364] | 2 | EBI-371008,EBI-1126877 |
ftsA [P0ABH0] | 3 | EBI-371008,EBI-550562 |
ftsZ [P0A9A6] | 7 | EBI-371008,EBI-370963 |
itself | 3 | EBI-371008,EBI-371008 |
rodZ [P27434] | 3 | EBI-371008,EBI-1129185 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261940, 383 interactors |
ComplexPortali | CPX-5718, Elongasome complex |
DIPi | DIP-31874N |
IntActi | P0A9X4, 82 interactors |
MINTi | P0A9X4 |
STRINGi | 511145.b3251 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1077, Bacteria |
HOGENOMi | CLU_052037_0_0_6 |
InParanoidi | P0A9X4 |
PhylomeDBi | P0A9X4 |
Family and domain databases
CDDi | cd10225, MreB_like, 1 hit |
HAMAPi | MF_02207, MreB, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR004753, MreB |
PRINTSi | PR01652, SHAPEPROTEIN |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR00904, mreB, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLKKFRGMFS NDLSIDLGTA NTLIYVKGQG IVLNEPSVVA IRQDRAGSPK
60 70 80 90 100
SVAAVGHDAK QMLGRTPGNI AAIRPMKDGV IADFFVTEKM LQHFIKQVHS
110 120 130 140 150
NSFMRPSPRV LVCVPVGATQ VERRAIRESA QGAGAREVFL IEEPMAAAIG
160 170 180 190 200
AGLPVSEATG SMVVDIGGGT TEVAVISLNG VVYSSSVRIG GDRFDEAIIN
210 220 230 240 250
YVRRNYGSLI GEATAERIKH EIGSAYPGDE VREIEVRGRN LAEGVPRGFT
260 270 280 290 300
LNSNEILEAL QEPLTGIVSA VMVALEQCPP ELASDISERG MVLTGGGALL
310 320 330 340
RNLDRLLMEE TGIPVVVAED PLTCVARGGG KALEMIDMHG GDLFSEE
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 – 61 | KQ → NE in AAA83891 (PubMed:3049542).Curated | 2 | |
Sequence conflicti | 265 | T → I in AAA83891 (PubMed:3049542).Curated | 1 | |
Sequence conflicti | 277 – 278 | QC → HT in AAA83891 (PubMed:3049542).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22055 Genomic DNA Translation: AAA83891.1 U18997 Genomic DNA Translation: AAA58054.1 Different initiation. U00096 Genomic DNA Translation: AAC76283.2 AP009048 Genomic DNA Translation: BAE77293.1 M31792 Genomic DNA Translation: AAA24154.1 |
RefSeqi | NP_417717.2, NC_000913.3 WP_000913396.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76283; AAC76283; b3251 BAE77293; BAE77293; BAE77293 |
GeneIDi | 64728064 67517865 948588 |
KEGGi | ecj:JW3220 eco:b3251 |
PATRICi | fig|511145.12.peg.3348 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22055 Genomic DNA Translation: AAA83891.1 U18997 Genomic DNA Translation: AAA58054.1 Different initiation. U00096 Genomic DNA Translation: AAC76283.2 AP009048 Genomic DNA Translation: BAE77293.1 M31792 Genomic DNA Translation: AAA24154.1 |
RefSeqi | NP_417717.2, NC_000913.3 WP_000913396.1, NZ_STEB01000012.1 |
3D structure databases
SMRi | P0A9X4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261940, 383 interactors |
ComplexPortali | CPX-5718, Elongasome complex |
DIPi | DIP-31874N |
IntActi | P0A9X4, 82 interactors |
MINTi | P0A9X4 |
STRINGi | 511145.b3251 |
Protein family/group databases
TCDBi | 9.B.157.1.3, the cell shape-determining mrebcd (mrebcd) family |
Proteomic databases
jPOSTi | P0A9X4 |
PaxDbi | P0A9X4 |
PRIDEi | P0A9X4 |
Genome annotation databases
EnsemblBacteriai | AAC76283; AAC76283; b3251 BAE77293; BAE77293; BAE77293 |
GeneIDi | 64728064 67517865 948588 |
KEGGi | ecj:JW3220 eco:b3251 |
PATRICi | fig|511145.12.peg.3348 |
Organism-specific databases
EchoBASEi | EB0603 |
Phylogenomic databases
eggNOGi | COG1077, Bacteria |
HOGENOMi | CLU_052037_0_0_6 |
InParanoidi | P0A9X4 |
PhylomeDBi | P0A9X4 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10608-MONOMER |
Miscellaneous databases
PROi | PR:P0A9X4 |
Family and domain databases
CDDi | cd10225, MreB_like, 1 hit |
HAMAPi | MF_02207, MreB, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR004753, MreB |
PRINTSi | PR01652, SHAPEPROTEIN |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR00904, mreB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MREB_ECOLI | |
Accessioni | P0A9X4Primary (citable) accession number: P0A9X4 Secondary accession number(s): P13519, P76678, Q2M8W3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | September 13, 2005 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families