UniProtKB - P0A9S1 (FUCO_ECOLI)
Protein
Lactaldehyde reductase
Gene
fucO
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
Cofactori
Fe cation1 Publication
Activity regulationi
Inhibited by Zn2+.1 Publication
: L-fucose degradation Pathwayi
This protein is involved in the pathway L-fucose degradation, which is part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the pathway L-fucose degradation and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | NAD | 1 | |
Binding sitei | 70 | NAD | 1 | |
Binding sitei | 150 | NAD | 1 | |
Binding sitei | 161 | NAD | 1 | |
Metal bindingi | 195 | Iron | 1 | |
Metal bindingi | 199 | Iron; via tele nitrogen | 1 | |
Metal bindingi | 262 | Iron; via tele nitrogen | 1 | |
Metal bindingi | 276 | Iron; via tele nitrogen | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 97 – 98 | NAD | 2 | |
Nucleotide bindingi | 139 – 143 | NAD | 5 | |
Nucleotide bindingi | 180 – 184 | NAD | 5 |
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: GO_Central
- ferrous iron binding Source: EcoCyc
- lactaldehyde reductase activity Source: EcoCyc
GO - Biological processi
- glycol catabolic process Source: EcoCyc
- L-fucose catabolic process Source: EcoCyc
- propanediol metabolic process Source: EcoCyc
- rhamnose catabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Carbohydrate metabolism, Fucose metabolism |
Ligand | Iron, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:LACTALDREDUCT-MONOMER MetaCyc:LACTALDREDUCT-MONOMER |
UniPathwayi | UPA00563 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fucO Ordered Locus Names:b2799, JW2770 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 9 | MANRMILNE → M: Loss of enzyme activity, loss of dimerization. 1 Publication | 9 | |
Mutagenesisi | 16 | G → D: No effect on enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 38 | D → G: Enzyme can now use NADP. 1 Publication | 1 | |
Mutagenesisi | 96 | G → E: Loss of NAD binding and enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 195 | D → L: Complete loss of iron-binding. 1 Publication | 1 | |
Mutagenesisi | 199 | H → A or F: Complete loss of iron-binding. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB02059, Adenosine-5-Diphosphoribose |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087824 | 1 – 382 | Lactaldehyde reductaseAdd BLAST | 382 |
Proteomic databases
jPOSTi | P0A9S1 |
PaxDbi | P0A9S1 |
PRIDEi | P0A9S1 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259223, 91 interactors 851603, 2 interactors |
DIPi | DIP-48076N |
IntActi | P0A9S1, 5 interactors |
STRINGi | 511145.b2799 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A9S1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A9S1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1454, Bacteria |
HOGENOMi | CLU_007207_0_0_6 |
InParanoidi | P0A9S1 |
Family and domain databases
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR018211, ADH_Fe_CS IPR039697, Alcohol_dehydrogenase_Fe IPR013460, Lactal_redase |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
TIGRFAMsi | TIGR02638, lactal_redase, 1 hit |
PROSITEi | View protein in PROSITE PS00913, ADH_IRON_1, 1 hit PS00060, ADH_IRON_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9S1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANRMILNET AWFGRGAVGA LTDEVKRRGY QKALIVTDKT LVQCGVVAKV
60 70 80 90 100
TDKMDAAGLA WAIYDGVVPN PTITVVKEGL GVFQNSGADY LIAIGGGSPQ
110 120 130 140 150
DTCKAIGIIS NNPEFADVRS LEGLSPTNKP SVPILAIPTT AGTAAEVTIN
160 170 180 190 200
YVITDEEKRR KFVCVDPHDI PQVAFIDADM MDGMPPALKA ATGVDALTHA
210 220 230 240 250
IEGYITRGAW ALTDALHIKA IEIIAGALRG SVAGDKDAGE EMALGQYVAG
260 270 280 290 300
MGFSNVGLGL VHGMAHPLGA FYNTPHGVAN AILLPHVMRY NADFTGEKYR
310 320 330 340 350
DIARVMGVKV EGMSLEEARN AAVEAVFALN RDVGIPPHLR DVGVRKEDIP
360 370 380
ALAQAALDDV CTGGNPREAT LEDIVELYHT AW
Sequence cautioni
The sequence AAA23824 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAA23825 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAB40449 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAE76871 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA33124 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 273 | N → T in CAA33124 (PubMed:2664711).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31059 Genomic DNA Translation: AAA23824.1 Different initiation. X15025 Genomic DNA Translation: CAA33124.1 Different initiation. M27177 Genomic DNA Translation: AAA23825.1 Different initiation. U29581 Genomic DNA Translation: AAB40449.1 Different initiation. U00096 Genomic DNA Translation: AAC75841.2 AP009048 Genomic DNA Translation: BAE76871.1 Different initiation. L07763 Genomic DNA No translation available. |
PIRi | A32883, RDECLA |
RefSeqi | NP_417279.2, NC_000913.3 WP_000013588.1, NZ_STEB01000030.1 |
Genome annotation databases
EnsemblBacteriai | AAC75841; AAC75841; b2799 BAE76871; BAE76871; BAE76871 |
GeneIDi | 947273 |
KEGGi | ecj:JW2770 eco:b2799 |
PATRICi | fig|1411691.4.peg.3934 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31059 Genomic DNA Translation: AAA23824.1 Different initiation. X15025 Genomic DNA Translation: CAA33124.1 Different initiation. M27177 Genomic DNA Translation: AAA23825.1 Different initiation. U29581 Genomic DNA Translation: AAB40449.1 Different initiation. U00096 Genomic DNA Translation: AAC75841.2 AP009048 Genomic DNA Translation: BAE76871.1 Different initiation. L07763 Genomic DNA No translation available. |
PIRi | A32883, RDECLA |
RefSeqi | NP_417279.2, NC_000913.3 WP_000013588.1, NZ_STEB01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RRM | X-ray | 1.60 | A/B | 1-382 | [»] | |
2BI4 | X-ray | 2.85 | A/B | 1-382 | [»] | |
2BL4 | X-ray | 2.85 | A/B | 1-382 | [»] | |
5BR4 | X-ray | 0.91 | A/B | 1-382 | [»] | |
SMRi | P0A9S1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259223, 91 interactors 851603, 2 interactors |
DIPi | DIP-48076N |
IntActi | P0A9S1, 5 interactors |
STRINGi | 511145.b2799 |
Chemistry databases
DrugBanki | DB02059, Adenosine-5-Diphosphoribose |
Proteomic databases
jPOSTi | P0A9S1 |
PaxDbi | P0A9S1 |
PRIDEi | P0A9S1 |
Genome annotation databases
EnsemblBacteriai | AAC75841; AAC75841; b2799 BAE76871; BAE76871; BAE76871 |
GeneIDi | 947273 |
KEGGi | ecj:JW2770 eco:b2799 |
PATRICi | fig|1411691.4.peg.3934 |
Organism-specific databases
EchoBASEi | EB0347 |
Phylogenomic databases
eggNOGi | COG1454, Bacteria |
HOGENOMi | CLU_007207_0_0_6 |
InParanoidi | P0A9S1 |
Enzyme and pathway databases
UniPathwayi | UPA00563 |
BioCyci | EcoCyc:LACTALDREDUCT-MONOMER MetaCyc:LACTALDREDUCT-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A9S1 |
PROi | PR:P0A9S1 |
Family and domain databases
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR018211, ADH_Fe_CS IPR039697, Alcohol_dehydrogenase_Fe IPR013460, Lactal_redase |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
TIGRFAMsi | TIGR02638, lactal_redase, 1 hit |
PROSITEi | View protein in PROSITE PS00913, ADH_IRON_1, 1 hit PS00060, ADH_IRON_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FUCO_ECOLI | |
Accessioni | P0A9S1Primary (citable) accession number: P0A9S1 Secondary accession number(s): P11549, Q2MA35 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | November 16, 2011 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families