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UniProtKB - P0A9R4 (FER_ECOLI)
Protein
2Fe-2S ferredoxin
Gene
fdx
Organism
Escherichia coli (strain K12)
Status
Functioni
Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.
Cofactori
[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 42 | Iron-sulfur (2Fe-2S) | 1 | |
Metal bindingi | 48 | Iron-sulfur (2Fe-2S) | 1 | |
Metal bindingi | 51 | Iron-sulfur (2Fe-2S) | 1 | |
Metal bindingi | 87 | Iron-sulfur (2Fe-2S) | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: EcoCyc
- electron transfer activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- electron transport chain Source: GO_Central
- iron-sulfur cluster assembly Source: EcoCyc
- P450-containing electron transport chain Source: InterPro
Keywordsi
Biological process | Electron transport, Transport |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FERREDOXIN-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 2Fe-2S ferredoxin |
Gene namesi | Name:fdx Ordered Locus Names:b2525, JW2509 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000201168 | 2 – 111 | 2Fe-2S ferredoxinAdd BLAST | 110 |
Proteomic databases
jPOSTi | P0A9R4 |
PaxDbi | P0A9R4 |
PRIDEi | P0A9R4 |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4260584, 52 interactors 851492, 4 interactors |
DIPi | DIP-48512N |
IntActi | P0A9R4, 14 interactors |
STRINGi | 511145.b2525 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A9R4 |
BMRBi | P0A9R4 |
SMRi | P0A9R4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A9R4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 104 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 103 |
Sequence similaritiesi
Belongs to the adrenodoxin/putidaredoxin family.Curated
Phylogenomic databases
eggNOGi | COG0633, Bacteria |
HOGENOMi | CLU_082632_5_2_6 |
InParanoidi | P0A9R4 |
OMAi | SACGGVC |
PhylomeDBi | P0A9R4 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR001055, Adrenodoxin IPR018298, Adrenodoxin_Fe-S_BS IPR012675, Beta-grasp_dom_sf IPR011536, Fdx_isc |
PANTHERi | PTHR23426, PTHR23426, 1 hit |
Pfami | View protein in Pfam PF00111, Fer2, 1 hit |
PRINTSi | PR00355, ADRENODOXIN |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR02007, fdx_isc, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS00814, ADX, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A9R4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKIVILPHQ DLCPDGAVLE ANSGETILDA ALRNGIEIEH ACEKSCACTT
60 70 80 90 100
CHCIVREGFD SLPESSEQED DMLDKAWGLE PESRLSCQAR VTDEDLVVEI
110
PRYTINHARE H
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | P → Y AA sequence (PubMed:4375562).Curated | 1 | |
Sequence conflicti | 63 | P → Q in U01827 (PubMed:8300516).Curated | 1 | |
Sequence conflicti | 68 – 69 | QE → HQ in U01827 (PubMed:8300516).Curated | 2 | |
Sequence conflicti | 74 | D → N in U01827 (PubMed:8300516).Curated | 1 | |
Sequence conflicti | 77 | W → R in U01827 (PubMed:8300516).Curated | 1 | |
Sequence conflicti | 91 – 93 | VTD → SYH in U01827 (PubMed:8300516).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88654 Genomic DNA Translation: AAA23755.1 U05338 Genomic DNA Translation: AAD13474.1 U01827 Unassigned DNA No translation available. U00096 Genomic DNA Translation: AAC75578.1 AP009048 Genomic DNA Translation: BAA16415.1 |
PIRi | JC1110 |
RefSeqi | NP_417020.1, NC_000913.3 WP_001124469.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75578; AAC75578; b2525 BAA16415; BAA16415; BAA16415 |
GeneIDi | 67416909 947160 |
KEGGi | ecj:JW2509 eco:b2525 |
PATRICi | fig|1411691.4.peg.4209 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88654 Genomic DNA Translation: AAA23755.1 U05338 Genomic DNA Translation: AAD13474.1 U01827 Unassigned DNA No translation available. U00096 Genomic DNA Translation: AAC75578.1 AP009048 Genomic DNA Translation: BAA16415.1 |
PIRi | JC1110 |
RefSeqi | NP_417020.1, NC_000913.3 WP_001124469.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I7H | X-ray | 1.70 | A/B/C | 1-111 | [»] | |
AlphaFoldDBi | P0A9R4 | |||||
BMRBi | P0A9R4 | |||||
SMRi | P0A9R4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260584, 52 interactors 851492, 4 interactors |
DIPi | DIP-48512N |
IntActi | P0A9R4, 14 interactors |
STRINGi | 511145.b2525 |
Proteomic databases
jPOSTi | P0A9R4 |
PaxDbi | P0A9R4 |
PRIDEi | P0A9R4 |
Genome annotation databases
EnsemblBacteriai | AAC75578; AAC75578; b2525 BAA16415; BAA16415; BAA16415 |
GeneIDi | 67416909 947160 |
KEGGi | ecj:JW2509 eco:b2525 |
PATRICi | fig|1411691.4.peg.4209 |
Organism-specific databases
EchoBASEi | EB1304 |
Phylogenomic databases
eggNOGi | COG0633, Bacteria |
HOGENOMi | CLU_082632_5_2_6 |
InParanoidi | P0A9R4 |
OMAi | SACGGVC |
PhylomeDBi | P0A9R4 |
Enzyme and pathway databases
BioCyci | EcoCyc:FERREDOXIN-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A9R4 |
PROi | PR:P0A9R4 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR001055, Adrenodoxin IPR018298, Adrenodoxin_Fe-S_BS IPR012675, Beta-grasp_dom_sf IPR011536, Fdx_isc |
PANTHERi | PTHR23426, PTHR23426, 1 hit |
Pfami | View protein in Pfam PF00111, Fer2, 1 hit |
PRINTSi | PR00355, ADRENODOXIN |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR02007, fdx_isc, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS00814, ADX, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FER_ECOLI | |
Accessioni | P0A9R4Primary (citable) accession number: P0A9R4 Secondary accession number(s): P25528 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families