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Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi34Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi37Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygen1 Publication1
Binding sitei203S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen1 Publication1

GO - Molecular functioni

  • [formate-C-acetyltransferase]-activating enzyme activity Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • enzyme active site formation Source: EcoCyc
  • glucose metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:PFLACTENZ-MONOMER
BRENDAi1.97.1.4 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Synonyms:act
Ordered Locus Names:b0902, JW0885
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10028 pflA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002005222 – 246Pyruvate formate-lyase 1-activating enzymeAdd BLAST245

Proteomic databases

EPDiP0A9N4
PaxDbiP0A9N4
PRIDEiP0A9N4

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ydcYP644554EBI-1114060,EBI-9129853

Protein-protein interaction databases

BioGridi4259353, 13 interactors
DIPiDIP-35915N
IntActiP0A9N4, 19 interactors
STRINGi316385.ECDH10B_0972

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 14Combined sources10
Beta strandi18 – 28Combined sources11
Helixi40 – 42Combined sources3
Beta strandi48 – 51Combined sources4
Helixi53 – 60Combined sources8
Helixi61 – 63Combined sources3
Helixi64 – 67Combined sources4
Beta strandi73 – 79Combined sources7
Helixi81 – 84Combined sources4
Helixi85 – 96Combined sources12
Turni97 – 99Combined sources3
Beta strandi102 – 106Combined sources5
Helixi115 – 122Combined sources8
Beta strandi125 – 130Combined sources6
Helixi136 – 143Combined sources8
Helixi148 – 160Combined sources13
Beta strandi164 – 170Combined sources7
Turni172 – 174Combined sources3
Helixi178 – 191Combined sources14
Beta strandi194 – 201Combined sources8
Helixi207 – 212Combined sources6
Turni218 – 221Combined sources4
Helixi227 – 238Combined sources12
Turni239 – 241Combined sources3

3D structure databases

ProteinModelPortaliP0A9N4
SMRiP0A9N4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9N4

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 38S-adenosyl-L-methionine binding1 Publication3
Regioni130 – 132S-adenosyl-L-methionine binding1 Publication3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A Bacteria
COG1180 LUCA
HOGENOMiHOG000011458
InParanoidiP0A9N4
KOiK04069
OMAiIGVPNKR
PhylomeDBiP0A9N4

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR034457 Organic_radical-activating
IPR012839 Organic_radical_activase
IPR012838 PFL1_activating
IPR034465 Pyruvate_for-lyase_activase
IPR001989 Radical_activat_CS
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000371 PFL_act_enz, 1 hit
SFLDiSFLDF00278 pyruvate_formate-lyase_activas, 1 hit
SFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR02493 PFLA, 1 hit
PROSITEiView protein in PROSITE
PS01087 RADICAL_ACTIVATING, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIGRIHSF ESCGTVDGPG IRFITFFQGC LMRCLYCHNR DTWDTHGGKE
60 70 80 90 100
VTVEDLMKEV VTYRHFMNAS GGGVTASGGE AILQAEFVRD WFRACKKEGI
110 120 130 140 150
HTCLDTNGFV RRYDPVIDEL LEVTDLVMLD LKQMNDEIHQ NLVGVSNHRT
160 170 180 190 200
LEFAKYLANK NVKVWIRYVV VPGWSDDDDS AHRLGEFTRD MGNVEKIELL
210 220 230 240
PYHELGKHKW VAMGEEYKLD GVKPPKKETM ERVKGILEQY GHKVMF
Length:246
Mass (Da):28,204
Last modified:January 23, 2007 - v2
Checksum:i486E06A9CE97BF37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08035 Genomic DNA Translation: CAA30829.1
U00096 Genomic DNA Translation: AAC73988.1
AP009048 Genomic DNA Translation: BAA35637.1
PIRiS01789
RefSeqiNP_415422.1, NC_000913.3
WP_000111043.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73988; AAC73988; b0902
BAA35637; BAA35637; BAA35637
GeneIDi945517
KEGGiecj:JW0885
eco:b0902
PATRICifig|1411691.4.peg.1374

Entry informationi

Entry nameiPFLA_ECOLI
AccessioniPrimary (citable) accession number: P0A9N4
Secondary accession number(s): P09374
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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