pta

Functionⁱ

Involved in acetate metabolism. Catalyzes the reversible interconversion of acetyl-CoA and acetyl phosphate. The direction of the overall reaction changes depending on growth conditions. On minimal medium acetyl-CoA is generated. In rich medium acetyl-CoA is converted to acetate and allowing the cell to dump the excess of acetylation potential in exchange for energy in the form of ATP.1 Publication

Catalytic activityⁱ

Acetyl-CoA + phosphate = CoA + acetyl phosphate.

Activity regulationⁱ

Inhibited by NADH and ATP. Pyruvate and PEP act as activators of the acetyl phosphate forming reaction while inhibiting the formation of acetyl-CoA.1 Publication

Kineticsⁱ

Pathwayⁱ: acetyl-CoA biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes acetyl-CoA from acetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Acetate kinase (ackA)
Phosphate acetyltransferase (pta)

This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functionⁱ

phosphate acetyltransferase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 13535743
- 20236319
zinc ion binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 11985624

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

acetate biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 21941
acetate catabolic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 21941
acetate metabolic process
Source: CACAO
Inferred from mutant phenotypeⁱ
- 21941
acetyl-CoA biosynthetic process from acetate
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 21941
L-threonine catabolic process to propionate
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 9484901

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Acyltransferase, Transferase

Molecular function

Acyltransferase, Transferase

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PHOSACETYLTRANS-MONOMER MetaCyc:PHOSACETYLTRANS-MONOMER
BRENDAⁱ	2.3.1.8 2026
UniPathwayⁱ	UPA00340;UER00459

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phosphate acetyltransferase (EC:2.3.1.8) Alternative name(s): Phosphotransacetylase
Gene namesⁱ	Name:pta Ordered Locus Names:b2297, JW2294
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG20173 pta

EcoGeneⁱ

EG20173 pta

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Severe impairment of growth in anaerobic conditions, as well as in growth on minimal medium. Increased sensitivity to environmental changes. Poor starvation survival and slower growth on glucose, fructose, tryptone broth, or pyruvate, but normal growth on glycerol or succinate.2 Publications

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed2 Publications
ChainⁱPRO_0000179129	2 – 714	Phosphate acetyltransferaseAdd BLAST		713

Proteomic databases

EPDⁱ	P0A9M8
PaxDbⁱ	P0A9M8
PRIDEⁱ	P0A9M8

2D gel databases

SWISS-2DPAGEⁱ	P0A9M8

SWISS-2DPAGEⁱ

P0A9M8

Interactionⁱ

Subunit structureⁱ

Homohexamer.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
chpS	P08365	3	EBI-555015,EBI-556499
napD	P0A9I5	3	EBI-555015,EBI-554985
yjgH	P39332	2	EBI-555015,EBI-561380

Protein-protein interaction databases

BioGridⁱ	4261500, 351 interactors
Database of interacting proteins More...DIPⁱ	DIP-35815N
IntActⁱ	P0A9M8, 28 interactors
STRINGⁱ	316385.ECDH10B_2459

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0A9M8
SMRⁱ	P0A9M8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	391 – 714	Phosphate acetyltransferaseAdd BLAST		324

Domainⁱ

The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site.1 Publication

Sequence similaritiesⁱ

In the N-terminal section; belongs to the CobB/CobQ family.Curated

In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105C6K Bacteria COG0280 LUCA COG0857 LUCA
HOGENOMⁱ	HOG000053797
InParanoidⁱ	P0A9M8
KEGG Orthology (KO) More...KOⁱ	K13788
OMAⁱ	TPLMFEY
PhylomeDBⁱ	P0A9M8

Family and domain databases

Gene3Dⁱ	3.40.1390.20, 1 hit
InterProⁱ	View protein in InterPro IPR010766 DRTGG IPR016475 P-Actrans_bac IPR027417 P-loop_NTPase IPR004614 P_AcTrfase IPR002505 PTA_PTB IPR028979 Ser_kin/Pase_Hpr-like_N_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07085 DRTGG, 1 hit PF01515 PTA_PTB, 1 hit
PIRSFⁱ	PIRSF006107 PhpActrans_proteobac, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF75138 SSF75138, 1 hit
TIGRFAMsⁱ	TIGR00651 pta, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P0A9M8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSRIIMLIPT GTSVGLTSVS LGVIRAMERK GVRLSVFKPI AQPRTGGDAP 
        60         70         80         90        100
DQTTTIVRAN SSTTTAAEPL KMSYVEGLLS SNQKDVLMEE IVANYHANTK 
       110        120        130        140        150
DAEVVLVEGL VPTRKHQFAQ SLNYEIAKTL NAEIVFVMSQ GTDTPEQLKE 
       160        170        180        190        200
RIELTRNSFG GAKNTNITGV IVNKLNAPVD EQGRTRPDLS EIFDDSSKAK 
       210        220        230        240        250
VNNVDPAKLQ ESSPLPVLGA VPWSFDLIAT RAIDMARHLN ATIINEGDIN 
       260        270        280        290        300
TRRVKSVTFC ARSIPHMLEH FRAGSLLVTS ADRPDVLVAA CLAAMNGVEI 
       310        320        330        340        350
GALLLTGGYE MDARISKLCE RAFATGLPVF MVNTNTWQTS LSLQSFNLEV 
       360        370        380        390        400
PVDDHERIEK VQEYVANYIN ADWIESLTAT SERSRRLSPP AFRYQLTELA 
       410        420        430        440        450
RKAGKRIVLP EGDEPRTVKA AAICAERGIA TCVLLGNPAE INRVAASQGV 
       460        470        480        490        500
ELGAGIEIVD PEVVRESYVG RLVELRKNKG MTETVAREQL EDNVVLGTLM 
       510        520        530        540        550
LEQDEVDGLV SGAVHTTANT IRPPLQLIKT APGSSLVSSV FFMLLPEQVY 
       560        570        580        590        600
VYGDCAINPD PTAEQLAEIA IQSADSAAAF GIEPRVAMLS YSTGTSGAGS 
       610        620        630        640        650
DVEKVREATR LAQEKRPDLM IDGPLQYDAA VMADVAKSKA PNSPVAGRAT 
       660        670        680        690        700
VFIFPDLNTG NTTYKAVQRS ADLISIGPML QGMRKPVNDL SRGALVDDIV 
       710 
YTIALTAIQS AQQQ

Length:714

Mass (Da):77,172

Last modified:January 23, 2007 - v2

Checksum:ⁱB45AF0C93494A04C

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	20 – 50	SLGVI…GGDAP → AWRDPCNGTQRRSSERFQTY RSAAYRWRCA in BAA04663 (PubMed:7918659).CuratedAdd BLAST	31
Sequence conflictⁱ	208 – 209	KL → NV in BAA04502 (PubMed:7883769).Curated	2
Sequence conflictⁱ	208 – 209	KL → NV in BAA04663 (PubMed:7918659).Curated	2
Sequence conflictⁱ	263 – 271	SIPHMLEHF → QHSAHAGAL in BAA04663 (PubMed:7918659).Curated	9

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D17576 Genomic DNA Translation: BAA04502.1 D21123 Genomic DNA Translation: BAA04663.1 U00096 Genomic DNA Translation: AAC75357.1 AP009048 Genomic DNA Translation: BAA16136.1
PIRⁱ	G65001 JX0357 S50130
NCBI Reference Sequences More...RefSeqⁱ	NP_416800.1, NC_000913.3 WP_000086722.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75357; AAC75357; b2297 BAA16136; BAA16136; BAA16136
GeneIDⁱ	946778
KEGGⁱ	ecj:JW2294 eco:b2297
PATRICⁱ	fig\|1411691.4.peg.4437

Protein	Similar proteins		Species	Score	Length	Source
P0A9M8	Phosphate acetyltransferase		SHIFL		714	UniRef100_P0A9M8
Phosphate acetyltransferase		Escherichia coli NCCP15648		714
Phosphate acetyltransferase		Klebsiella pneumoniae IS22		714
Phosphate acetyltransferase		KLEPN		714
Phosphate acetyltransferase		Shigella flexneri 1235-66		714
+80

Protein	Similar proteins		Species	Score	Length	Source
P0A9M8	Phosphate acetyltransferase		SHIFL		714	UniRef90_P0A9M8
Phosphate acetyltransferase		SALTY		714
Phosphate acetyltransferase		Escherichia coli NCCP15648		714
Phosphate acetyltransferase		Klebsiella pneumoniae IS22		714
Phosphate acetyltransferase		KLEPN		714
+1393

Protein	Similar proteins		Species	Score	Length	Source
P0A9M8	Phosphate acetyltransferase		SHIFL		714	UniRef50_P0A9M8
Phosphate acetyltransferase		SALTY		714
Phosphate acetyltransferase		YERPE		717
Phosphate acetyltransferase		VIBCH		714
Phosphate acetyltransferase		Escherichia coli NCCP15648		714
+2427

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D17576 Genomic DNA Translation: BAA04502.1 D21123 Genomic DNA Translation: BAA04663.1 U00096 Genomic DNA Translation: AAC75357.1 AP009048 Genomic DNA Translation: BAA16136.1
PIRⁱ	G65001 JX0357 S50130
RefSeqⁱ	NP_416800.1, NC_000913.3 WP_000086722.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0A9M8
SMRⁱ	P0A9M8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261500, 351 interactors
DIPⁱ	DIP-35815N
IntActⁱ	P0A9M8, 28 interactors
STRINGⁱ	316385.ECDH10B_2459

2D gel databases

SWISS-2DPAGEⁱ	P0A9M8

SWISS-2DPAGEⁱ

P0A9M8

Proteomic databases

EPDⁱ	P0A9M8
PaxDbⁱ	P0A9M8
PRIDEⁱ	P0A9M8

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75357; AAC75357; b2297 BAA16136; BAA16136; BAA16136
GeneIDⁱ	946778
KEGGⁱ	ecj:JW2294 eco:b2297
PATRICⁱ	fig\|1411691.4.peg.4437

Organism-specific databases

EchoBASEⁱ	EB4147
EcoGeneⁱ	EG20173 pta

Phylogenomic databases

eggNOGⁱ	ENOG4105C6K Bacteria COG0280 LUCA COG0857 LUCA
HOGENOMⁱ	HOG000053797
InParanoidⁱ	P0A9M8
KOⁱ	K13788
OMAⁱ	TPLMFEY
PhylomeDBⁱ	P0A9M8

Enzyme and pathway databases

UniPathwayⁱ	UPA00340;UER00459
BioCycⁱ	EcoCyc:PHOSACETYLTRANS-MONOMER MetaCyc:PHOSACETYLTRANS-MONOMER
BRENDAⁱ	2.3.1.8 2026

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0A9M8

PROⁱ

PR:P0A9M8

Family and domain databases

Gene3Dⁱ	3.40.1390.20, 1 hit
InterProⁱ	View protein in InterPro IPR010766 DRTGG IPR016475 P-Actrans_bac IPR027417 P-loop_NTPase IPR004614 P_AcTrfase IPR002505 PTA_PTB IPR028979 Ser_kin/Pase_Hpr-like_N_sf
Pfamⁱ	View protein in Pfam PF07085 DRTGG, 1 hit PF01515 PTA_PTB, 1 hit
PIRSFⁱ	PIRSF006107 PhpActrans_proteobac, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF75138 SSF75138, 1 hit
TIGRFAMsⁱ	TIGR00651 pta, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PTA_ECOLI
Accessionⁱ	P0A9M8Primary (citable) accession number: P0A9M8 Secondary accession number(s): P39184 , P78091, P78189, P78190, Q9EUP2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
	Last sequence update:	January 23, 2007
	Last modified:	September 12, 2018
	This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P0A9M8 (PTA_ECOLI)

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Phosphate acetyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: acetyl-CoA biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

Pathwayⁱ: acetyl-CoA biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ