hpt

Functionⁱ

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine.1 Publication

Catalytic activityⁱ

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Cofactorⁱ

Mg²⁺By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayⁱ: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:

Hypoxanthine phosphoribosyltransferase (hpt)

This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	103	Proton acceptorCurated	1
Binding siteⁱ	131	IMP	1
Binding siteⁱ	153	IMP; via carbonyl oxygen	1
Metal bindingⁱ	159	MagnesiumBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	99 – 108	IMP		10
Nucleotide bindingⁱ	158 – 159	IMP		2

GO - Molecular functionⁱ

guanine phosphoribosyltransferase activity Source: UniProtKB-EC
hypoxanthine phosphoribosyltransferase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 12070315
magnesium ion binding
Source: EcoCyc
Inferred from direct assayⁱ
- 692401
nucleotide binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

GMP salvage
Source: EcoliWiki
Inferred from direct assayⁱ
- 12070315
IMP salvage
Source: EcoliWiki
Inferred from direct assayⁱ
- 12070315

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Purine salvage
Ligand	Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER
UniPathwayⁱ	UPA00591; UER00648

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8) Short name: HPRT
Gene namesⁱ	Name:hpt Ordered Locus Names:b0125, JW5009
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG20098 hpt

EcoGeneⁱ

EG20098 hpt

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB04566 Inosinic Acid

DrugBankⁱ

DB04566 Inosinic Acid

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000139632	1 – 178	Hypoxanthine phosphoribosyltransferaseAdd BLAST		178

Proteomic databases

EPDⁱ	P0A9M2
PaxDbⁱ	P0A9M2
PRIDEⁱ	P0A9M2

2D gel databases

SWISS-2DPAGEⁱ	P0A9M2

SWISS-2DPAGEⁱ

P0A9M2

Interactionⁱ

Subunit structureⁱ

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridⁱ	4261679, 26 interactors
Database of interacting proteins More...DIPⁱ	DIP-47994N
IntActⁱ	P0A9M2, 5 interactors
STRINGⁱ	316385.ECDH10B_0105

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	3 – 9	Combined sources	7
Helixⁱ	11 – 29	Combined sources	19
Turnⁱ	30 – 32	Combined sources	3
Beta strandⁱ	36 – 41	Combined sources	6
Turnⁱ	42 – 45	Combined sources	4
Helixⁱ	46 – 54	Combined sources	9
Beta strandⁱ	61 – 67	Combined sources	7
Turnⁱ	73 – 76	Combined sources	4
Beta strandⁱ	82 – 84	Combined sources	3
Beta strandⁱ	93 – 104	Combined sources	12
Helixⁱ	106 – 116	Combined sources	11
Beta strandⁱ	121 – 130	Combined sources	10
Helixⁱ	132 – 134	Combined sources	3
Beta strandⁱ	142 – 147	Combined sources	6
Beta strandⁱ	153 – 155	Combined sources	3
Beta strandⁱ	160 – 162	Combined sources	3
Beta strandⁱ	168 – 175	Combined sources	8

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1G9S	X-ray	2.80	A/B	1-178	[»]
	1G9T	X-ray	2.80	A/B	1-178	[»]
	1GRV	X-ray	2.90	A/B	1-178	[»]
	5KNR	X-ray	2.86	A/B	1-178	[»]
	5KNS	X-ray	2.79	A/B	1-178	[»]
	5KNT	X-ray	2.55	A/B	1-178	[»]
	5KNU	X-ray	2.81	A/B	1-178	[»]
	5KNV	X-ray	2.86	A/B	1-178	[»]
	5KNX	X-ray	2.40	A/B	1-178	[»]
ProteinModelPortalⁱ	P0A9M2
SMRⁱ	P0A9M2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0A9M2

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108UGV Bacteria COG0634 LUCA
HOGENOMⁱ	HOG000236520
InParanoidⁱ	P0A9M2
KEGG Orthology (KO) More...KOⁱ	K00760
OMAⁱ	TMDWMAV
PhylomeDBⁱ	P0A9M2

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06223 PRTases_typeI, 1 hit
InterProⁱ	View protein in InterPro IPR005904 Hxn_phspho_trans IPR000836 PRibTrfase_dom IPR029057 PRTase-like
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00156 Pribosyltran, 1 hit
SUPFAMⁱ	SSF53271 SSF53271, 1 hit
TIGRFAMsⁱ	TIGR01203 HGPRTase, 1 hit
PROSITEⁱ	View protein in PROSITE PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0A9M2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSDMVLVG LLRGSFMFMA 
        60         70         80         90        100
DLCREVQVSH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED 
       110        120        130        140        150
IIDSGNTLSK VREILSLREP KSLAICTLLD KPSRREVNVP VEFIGFSIPD 
       160        170 
EFVVGYGIDY AQRYRHLPYI GKVILLDE

Length:178

Mass (Da):20,115

Last modified:July 19, 2005 - v1

Checksum:ⁱE1A75EB68231DC32

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC73236.2 AP009048 Genomic DNA Translation: BAB96700.2
NCBI Reference Sequences More...RefSeqⁱ	NP_414667.4, NC_000913.3 WP_000683335.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73236; AAC73236; b0125 BAB96700; BAB96700; BAB96700
GeneIDⁱ	946624
KEGGⁱ	ecj:JW5009 eco:b0125
PATRICⁱ	fig\|1411691.4.peg.2157

Protein	Similar proteins		Species	Score	Length
P0A9M2	Hypoxanthine phosphoribosyltransferase	)	SALTY		178
Hypoxanthine phosphoribosyltransferase		Salmonella enterica subsp. enterica serovar Concord		178
Hypoxanthine phosphoribosyltransferase		Salmonella enterica subsp. enterica serovar Heidelberg		178
Hypoxanthine phosphoribosyltransferase		SALET		178
Hypoxanthine phosphoribosyltransferase		SALCE		178
+524

Protein	Similar proteins		Species	Score	Length
P0A9M2	Hypoxanthine phosphoribosyltransferase	)	SALTY		178
Hypoxanthine phosphoribosyltransferase		Salmonella enterica subsp. enterica serovar Concord		178
Hypoxanthine phosphoribosyltransferase		Salmonella enterica subsp. enterica serovar Heidelberg		178
Hypoxanthine phosphoribosyltransferase		SALET		178
Hypoxanthine phosphoribosyltransferase		SALCE		178
+1431

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC73236.2 AP009048 Genomic DNA Translation: BAB96700.2
NCBI Reference Sequences More...RefSeqⁱ	NP_414667.4, NC_000913.3 WP_000683335.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1G9S	X-ray	2.80	A/B	1-178	[»]
	1G9T	X-ray	2.80	A/B	1-178	[»]
	1GRV	X-ray	2.90	A/B	1-178	[»]
	5KNR	X-ray	2.86	A/B	1-178	[»]
	5KNS	X-ray	2.79	A/B	1-178	[»]
	5KNT	X-ray	2.55	A/B	1-178	[»]
	5KNU	X-ray	2.81	A/B	1-178	[»]
	5KNV	X-ray	2.86	A/B	1-178	[»]
	5KNX	X-ray	2.40	A/B	1-178	[»]
ProteinModelPortalⁱ	P0A9M2
SMRⁱ	P0A9M2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261679, 26 interactors
Database of interacting proteins More...DIPⁱ	DIP-47994N
IntActⁱ	P0A9M2, 5 interactors
STRINGⁱ	316385.ECDH10B_0105

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB04566 Inosinic Acid

DrugBankⁱ

DB04566 Inosinic Acid

2D gel databases

SWISS-2DPAGEⁱ	P0A9M2

SWISS-2DPAGEⁱ

P0A9M2

Proteomic databases

EPDⁱ	P0A9M2
PaxDbⁱ	P0A9M2
PRIDEⁱ	P0A9M2

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73236; AAC73236; b0125 BAB96700; BAB96700; BAB96700
GeneIDⁱ	946624
KEGGⁱ	ecj:JW5009 eco:b0125
PATRICⁱ	fig\|1411691.4.peg.2157

Organism-specific databases

EchoBASEⁱ	EB4143
EcoGeneⁱ	EG20098 hpt

Phylogenomic databases

eggNOGⁱ	ENOG4108UGV Bacteria COG0634 LUCA
HOGENOMⁱ	HOG000236520
InParanoidⁱ	P0A9M2
KEGG Orthology (KO) More...KOⁱ	K00760
OMAⁱ	TMDWMAV
PhylomeDBⁱ	P0A9M2

Enzyme and pathway databases

UniPathwayⁱ	UPA00591; UER00648
BioCycⁱ	EcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P0A9M2
Protein Ontology More...PROⁱ	PR:P0A9M2

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06223 PRTases_typeI, 1 hit
InterProⁱ	View protein in InterPro IPR005904 Hxn_phspho_trans IPR000836 PRibTrfase_dom IPR029057 PRTase-like
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00156 Pribosyltran, 1 hit
SUPFAMⁱ	SSF53271 SSF53271, 1 hit
TIGRFAMsⁱ	TIGR01203 HGPRTase, 1 hit
PROSITEⁱ	View protein in PROSITE PS00103 PUR_PYR_PR_TRANSFER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HPRT_ECOLI
Accessionⁱ	P0A9M2Primary (citable) accession number: P0A9M2 Secondary accession number(s): P36766
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
	Last sequence update:	July 19, 2005
	Last modified:	March 28, 2018
	This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0A9M2 (HPRT_ECOLI)

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Hypoxanthine phosphoribosyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: IMP biosynthesis via salvage pathway

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: IMP biosynthesis via salvage pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ