UniProtKB - P0A9L8 (P5CR_ECOLI)
Protein
Pyrroline-5-carboxylate reductase
Gene
proC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. Does not catalyze the reverse reaction.UniRule annotation2 Publications
Miscellaneous
Can use either NADPH or NADH, but the activity observed with NADPH is severalfold greater.1 Publication
Catalytic activityi
Activity regulationi
Inhibited by p-chloromercuribenzoate.1 Publication
: L-proline biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.UniRule annotation2 PublicationsProteins known to be involved in this subpathway in this organism are:
- Pyrroline-5-carboxylate reductase (proC), Pyrroline-5-carboxylate reductase (proC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- pyrroline-5-carboxylate reductase activity Source: EcoCyc
GO - Biological processi
- L-proline biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Proline biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:PYRROLINECARBREDUCT-MONOMER MetaCyc:PYRROLINECARBREDUCT-MONOMER |
UniPathwayi | UPA00098;UER00361 |
Names & Taxonomyi
Protein namesi | Recommended name: Pyrroline-5-carboxylate reductaseUniRule annotation (EC:1.5.1.2UniRule annotation)Short name: P5C reductaseUniRule annotation Short name: P5CRUniRule annotation Alternative name(s): PCA reductaseUniRule annotation |
Gene namesi | Name:proCUniRule annotation Ordered Locus Names:b0386, JW0377 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000187289 | 1 – 269 | Pyrroline-5-carboxylate reductaseAdd BLAST | 269 |
Proteomic databases
jPOSTi | P0A9L8 |
PaxDbi | P0A9L8 |
PRIDEi | P0A9L8 |
Interactioni
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4263533, 36 interactors 849425, 1 interactor |
DIPi | DIP-47863N |
IntActi | P0A9L8, 4 interactors |
STRINGi | 511145.b0386 |
Family & Domainsi
Sequence similaritiesi
Belongs to the pyrroline-5-carboxylate reductase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0345, Bacteria |
HOGENOMi | CLU_042344_3_1_6 |
InParanoidi | P0A9L8 |
PhylomeDBi | P0A9L8 |
Family and domain databases
HAMAPi | MF_01925, P5C_reductase, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR028939, P5C_Rdtase_cat_N IPR029036, P5CR_dimer IPR000304, Pyrroline-COOH_reductase |
Pfami | View protein in Pfam PF03807, F420_oxidored, 1 hit PF14748, P5CR_dimer, 1 hit |
PIRSFi | PIRSF000193, Pyrrol-5-carb_rd, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00112, proC, 1 hit |
PROSITEi | View protein in PROSITE PS00521, P5CR, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9L8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG
60 70 80 90 100
INAAESAQEV AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV
110 120 130 140 150
TLDQLARALG HDRKIIRAMP NTPALVNAGM TSVTPNALVT PEDTADVLNI
160 170 180 190 200
FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF MFIEAMADAA VLGGMPRAQA
210 220 230 240 250
YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV RVLEEKGFRA
260
AVIEAMTKCM EKSEKLSKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01665 Genomic DNA Translation: AAA86433.1 U73857 Genomic DNA Translation: AAB18110.1 U00096 Genomic DNA Translation: AAC73489.1 AP009048 Genomic DNA Translation: BAE76167.1 |
PIRi | A00385, RDECC |
RefSeqi | NP_414920.1, NC_000913.3 WP_001295331.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73489; AAC73489; b0386 BAE76167; BAE76167; BAE76167 |
GeneIDi | 58389216 945034 |
KEGGi | ecj:JW0377 eco:b0386 |
PATRICi | fig|1411691.4.peg.1892 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01665 Genomic DNA Translation: AAA86433.1 U73857 Genomic DNA Translation: AAB18110.1 U00096 Genomic DNA Translation: AAC73489.1 AP009048 Genomic DNA Translation: BAE76167.1 |
PIRi | A00385, RDECC |
RefSeqi | NP_414920.1, NC_000913.3 WP_001295331.1, NZ_STEB01000007.1 |
3D structure databases
SMRi | P0A9L8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263533, 36 interactors 849425, 1 interactor |
DIPi | DIP-47863N |
IntActi | P0A9L8, 4 interactors |
STRINGi | 511145.b0386 |
Proteomic databases
jPOSTi | P0A9L8 |
PaxDbi | P0A9L8 |
PRIDEi | P0A9L8 |
Genome annotation databases
EnsemblBacteriai | AAC73489; AAC73489; b0386 BAE76167; BAE76167; BAE76167 |
GeneIDi | 58389216 945034 |
KEGGi | ecj:JW0377 eco:b0386 |
PATRICi | fig|1411691.4.peg.1892 |
Organism-specific databases
EchoBASEi | EB0762 |
Phylogenomic databases
eggNOGi | COG0345, Bacteria |
HOGENOMi | CLU_042344_3_1_6 |
InParanoidi | P0A9L8 |
PhylomeDBi | P0A9L8 |
Enzyme and pathway databases
UniPathwayi | UPA00098;UER00361 |
BioCyci | EcoCyc:PYRROLINECARBREDUCT-MONOMER MetaCyc:PYRROLINECARBREDUCT-MONOMER |
Miscellaneous databases
PROi | PR:P0A9L8 |
Family and domain databases
HAMAPi | MF_01925, P5C_reductase, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR028939, P5C_Rdtase_cat_N IPR029036, P5CR_dimer IPR000304, Pyrroline-COOH_reductase |
Pfami | View protein in Pfam PF03807, F420_oxidored, 1 hit PF14748, P5CR_dimer, 1 hit |
PIRSFi | PIRSF000193, Pyrrol-5-carb_rd, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00112, proC, 1 hit |
PROSITEi | View protein in PROSITE PS00521, P5CR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P5CR_ECOLI | |
Accessioni | P0A9L8Primary (citable) accession number: P0A9L8 Secondary accession number(s): P00373, Q2MC39 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 10, 2021 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families