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Entry version 108 (12 Aug 2020)
Sequence version 1 (01 Apr 1988)
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Protein

Phosphate-specific transport system accessory protein PhoU

Gene

phoU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Part of the phosphate (Pho) regulon, which plays a key role in phosphate homeostasis. Encoded together with proteins of the phosphate-specific transport (Pst) system in the polycistronic pstSCAB-phoU operon. PhoU is essential for the repression of the Pho regulon at high phosphate conditions. In this role, it may bind, possibly as a chaperone, to PhoR, PhoB or a PhoR-PhoB complex to promote dephosphorylation of phospho-PhoB, or inhibit formation of the PhoR-PhoB transitory complex. Is also part of complex networks important for bacterial virulence, tolerance to antibiotics and stress response.2 Publications

Caution

Was originally thought to be involved in phosphate transport.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processPhosphate transport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG10735-MONOMER
ECOL316407:JW3702-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Phosphate-specific transport system accessory protein PhoU
Short name:
Pst system accessory protein PhoU
Alternative name(s):
Negative regulator of Pho regulon
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:phoU
Synonyms:nmpA
Ordered Locus Names:b3724, JW3702
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the use of a protein as a pharmaceutical drug. It indicates the name of the drug, the name of the firm that commercializes it and explains in a few words in which context the drug is used. In some cases, drugs that are under development are also described.<p><a href='/help/pharmaceutical_use' target='_top'>More...</a></p>Pharmaceutical usei

May be a drug target for designing new drugs that kill persister bacteria for more effective control of bacterial infections.

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Produces high level of alkaline phosphatase (AP) when grown with excess phosphate (PubMed:1459954). No effect on phosphate uptake, but particular deletion mutants have a severe growth defect, which is largely alleviated by a compensatory mutation in the pstSCAB genes or in the phoRB operon (PubMed:8226621). Accumulates high levels of polyP, approximately 400 nmol of phosphate residues/mg of protein (PubMed:12147514). Higher susceptibility to a diverse range of antibiotics including ampicillin, norfloxacin and gentamicin, and stresses such as starvation, acid pH, heat, peroxide, weak acids and energy inhibitors, especially in stationary phase (PubMed:17420206). Metabolically hyperactive status of the cell showing increased expression of energy production genes, flagella and chemotaxis genes, and a defect in persister formation (PubMed:17420206). Cells transport phosphate via PstSCAB transporter system at approximately 20% higher rate and accumulate higher levels of the transporter and about 50% more phosphate in 12 minutes than wild-type cells in phosphate-replete medium (PubMed:19047379).5 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001551681 – 241Phosphate-specific transport system accessory protein PhoUAdd BLAST241

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P0A9K7

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0A9K7

PRoteomics IDEntifications database

More...
PRIDEi
P0A9K7

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expressed at higher levels under excess phosphate culture conditions (PubMed:6090402). Induced by phosphate starvation via the PhoR/PhoB two-component regulatory system (PubMed:18031348).2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

By similarity

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4262141, 51 interactors

Database of interacting proteins

More...
DIPi
DIP-10503N

Protein interaction database and analysis system

More...
IntActi
P0A9K7, 1 interactor

STRING: functional protein association networks

More...
STRINGi
511145.b3724

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0A9K7

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the PhoU family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0704, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_078518_2_1_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0A9K7

KEGG Orthology (KO)

More...
KOi
K02039

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0A9K7

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.58.220, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR028366, P_transport_PhoU
IPR038078, PhoU-like_sf
IPR026022, PhoU_dom

The PANTHER Classification System

More...
PANTHERi
PTHR42930:SF3, PTHR42930:SF3, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01895, PhoU, 2 hits

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF003107, PhoU, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02135, phoU_full, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0A9K7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSLNLNKHI SGQFNAELES IRTQVMTMGG MVEQQLSDAI TAMHNQDSDL
60 70 80 90 100
AKRVIEGDKN VNMMEVAIDE ACVRIIAKRQ PTASDLRLVM VISKTIAELE
110 120 130 140 150
RIGDVADKIC RTALEKFSQQ HQPLLVSLES LGRHTIQMLH DVLDAFARMD
160 170 180 190 200
IDEAVRIYRE DKKVDQEYEG IVRQLMTYMM EDSRTIPSVL TALFCARSIE
210 220 230 240
RIGDRCQNIC EFIFYYVKGQ DFRHVGGDEL DKLLAGKDSD K
Length:241
Mass (Da):27,417
Last modified:April 1, 1988 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i29843C1C3827FACD
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X02723 Genomic DNA Translation: CAA26510.1
K01992 Genomic DNA Translation: AAA24382.1
L10328 Genomic DNA Translation: AAA62075.1
U00096 Genomic DNA Translation: AAC76747.1
AP009048 Genomic DNA Translation: BAE77564.1
M16487 Genomic DNA Translation: AAA23507.1

Protein sequence database of the Protein Information Resource

More...
PIRi
D23311, BVECPU

NCBI Reference Sequences

More...
RefSeqi
NP_418180.1, NC_000913.3
WP_000377786.1, NZ_STEB01000015.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76747; AAC76747; b3724
BAE77564; BAE77564; BAE77564

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
48133531
948233

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3702
eco:b3724

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2976

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02723 Genomic DNA Translation: CAA26510.1
K01992 Genomic DNA Translation: AAA24382.1
L10328 Genomic DNA Translation: AAA62075.1
U00096 Genomic DNA Translation: AAC76747.1
AP009048 Genomic DNA Translation: BAE77564.1
M16487 Genomic DNA Translation: AAA23507.1
PIRiD23311, BVECPU
RefSeqiNP_418180.1, NC_000913.3
WP_000377786.1, NZ_STEB01000015.1

3D structure databases

SMRiP0A9K7
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262141, 51 interactors
DIPiDIP-10503N
IntActiP0A9K7, 1 interactor
STRINGi511145.b3724

Proteomic databases

jPOSTiP0A9K7
PaxDbiP0A9K7
PRIDEiP0A9K7

Genome annotation databases

EnsemblBacteriaiAAC76747; AAC76747; b3724
BAE77564; BAE77564; BAE77564
GeneIDi48133531
948233
KEGGiecj:JW3702
eco:b3724
PATRICifig|1411691.4.peg.2976

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0728

Phylogenomic databases

eggNOGiCOG0704, Bacteria
HOGENOMiCLU_078518_2_1_6
InParanoidiP0A9K7
KOiK02039
PhylomeDBiP0A9K7

Enzyme and pathway databases

BioCyciEcoCyc:EG10735-MONOMER
ECOL316407:JW3702-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P0A9K7

Family and domain databases

Gene3Di1.20.58.220, 2 hits
InterProiView protein in InterPro
IPR028366, P_transport_PhoU
IPR038078, PhoU-like_sf
IPR026022, PhoU_dom
PANTHERiPTHR42930:SF3, PTHR42930:SF3, 1 hit
PfamiView protein in Pfam
PF01895, PhoU, 2 hits
PIRSFiPIRSF003107, PhoU, 1 hit
TIGRFAMsiTIGR02135, phoU_full, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPHOU_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0A9K7
Secondary accession number(s): P07656, Q2M842
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: August 12, 2020
This is version 108 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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