UniProtKB - P0A9I8 (NIRD_ECOLI)
Protein
Nitrite reductase (NADH) small subunit
Gene
nirD
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for activity of the reductase.1 Publication
Catalytic activityi
- EC:1.7.1.15
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: InterPro
- nitrite reductase [NAD(P)H] activity Source: EcoCyc
- nitrite reductase NADH activity Source: RHEA
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- nitrate assimilation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrate assimilation |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:NIRD-MONOMER MetaCyc:NIRD-MONOMER |
BRENDAi | 1.7.1.15, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nirD Ordered Locus Names:b3366, JW3329 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
- nitrite reductase complex [NAD(P)H] Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096855 | 1 – 108 | Nitrite reductase (NADH) small subunitAdd BLAST | 108 |
Proteomic databases
jPOSTi | P0A9I8 |
PaxDbi | P0A9I8 |
PRIDEi | P0A9I8 |
Interactioni
Subunit structurei
Associates with NirB.
Protein-protein interaction databases
BioGRIDi | 4262476, 15 interactors |
STRINGi | 511145.b3366 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A9I8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A9I8 |
Family & Domainsi
Sequence similaritiesi
To B.subtilis NasE.Curated
Phylogenomic databases
eggNOGi | COG2146, Bacteria |
HOGENOMi | CLU_055690_3_0_6 |
InParanoidi | P0A9I8 |
PhylomeDBi | P0A9I8 |
Family and domain databases
CDDi | cd03529, Rieske_NirD, 1 hit |
Gene3Di | 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR017881, NirD IPR012748, Rieske-like_NirD IPR036922, Rieske_2Fe-2S_sf |
PANTHERi | PTHR40562, PTHR40562, 1 hit |
Pfami | View protein in Pfam PF13806, Rieske_2, 1 hit |
SUPFAMi | SSF50022, SSF50022, 1 hit |
TIGRFAMsi | TIGR02378, nirD_assim_sml, 1 hit |
PROSITEi | View protein in PROSITE PS51300, NIRD, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9I8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE
60 70 80 90 100
SSVLSRGLIA EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK
DGVVQLRG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | G → D (PubMed:2543955).Curated | 1 | |
Sequence conflicti | 102 | G → D (PubMed:2200672).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14202 Genomic DNA Translation: CAA32417.1 Sequence problems. U18997 Genomic DNA Translation: AAA58163.1 U00096 Genomic DNA Translation: AAC76391.1 AP009048 Genomic DNA Translation: BAE77924.1 |
PIRi | A65131 |
RefSeqi | NP_417825.1, NC_000913.3 WP_000084764.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76391; AAC76391; b3366 BAE77924; BAE77924; BAE77924 |
GeneIDi | 58388152 947881 |
KEGGi | ecj:JW3329 eco:b3366 |
PATRICi | fig|1411691.4.peg.3363 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14202 Genomic DNA Translation: CAA32417.1 Sequence problems. U18997 Genomic DNA Translation: AAA58163.1 U00096 Genomic DNA Translation: AAC76391.1 AP009048 Genomic DNA Translation: BAE77924.1 |
PIRi | A65131 |
RefSeqi | NP_417825.1, NC_000913.3 WP_000084764.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JO6 | NMR | - | A | 1-108 | [»] | |
SMRi | P0A9I8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262476, 15 interactors |
STRINGi | 511145.b3366 |
Proteomic databases
jPOSTi | P0A9I8 |
PaxDbi | P0A9I8 |
PRIDEi | P0A9I8 |
Protocols and materials databases
DNASUi | 947881 |
Genome annotation databases
EnsemblBacteriai | AAC76391; AAC76391; b3366 BAE77924; BAE77924; BAE77924 |
GeneIDi | 58388152 947881 |
KEGGi | ecj:JW3329 eco:b3366 |
PATRICi | fig|1411691.4.peg.3363 |
Organism-specific databases
EchoBASEi | EB0649 |
Phylogenomic databases
eggNOGi | COG2146, Bacteria |
HOGENOMi | CLU_055690_3_0_6 |
InParanoidi | P0A9I8 |
PhylomeDBi | P0A9I8 |
Enzyme and pathway databases
BioCyci | EcoCyc:NIRD-MONOMER MetaCyc:NIRD-MONOMER |
BRENDAi | 1.7.1.15, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A9I8 |
PROi | PR:P0A9I8 |
Family and domain databases
CDDi | cd03529, Rieske_NirD, 1 hit |
Gene3Di | 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR017881, NirD IPR012748, Rieske-like_NirD IPR036922, Rieske_2Fe-2S_sf |
PANTHERi | PTHR40562, PTHR40562, 1 hit |
Pfami | View protein in Pfam PF13806, Rieske_2, 1 hit |
SUPFAMi | SSF50022, SSF50022, 1 hit |
TIGRFAMsi | TIGR02378, nirD_assim_sml, 1 hit |
PROSITEi | View protein in PROSITE PS51300, NIRD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIRD_ECOLI | |
Accessioni | P0A9I8Primary (citable) accession number: P0A9I8 Secondary accession number(s): P23675, Q2M732 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references