UniProtKB - P0A9H9 (CHEZ_ECOLI)
Protein
Protein phosphatase CheZ
Gene
cheZ
Organism
Escherichia coli (strain K12)
Status
Functioni
Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 147 | Enhances dephosphorylation of CheY-P | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- phosphoprotein phosphatase activity Source: EcoCyc
GO - Biological processi
- bacterial-type flagellum-dependent swarming motility Source: EcoCyc
- chemotaxis Source: EcoCyc
- protein dephosphorylation Source: EcoCyc
- regulation of chemotaxis Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Chemotaxis, Flagellar rotation |
Enzyme and pathway databases
BioCyci | EcoCyc:CHEZ-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cheZ Ordered Locus Names:b1881, JW1870 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication Note: Colocalizes with CheA chemoreceptor patches near the cell poles, which requires CheA(short).
GO - Cellular componenti
- bacterial-type flagellum Source: InterPro
- cytosol Source: EcoCyc
- methyl accepting chemotaxis protein complex Source: CAFA
- plasma membrane Source: CAFA
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | I → T: Gain of function, increased counterclockwise rotation of flagella. 1 Publication | 1 | |
Mutagenesisi | 65 | A → V: Loss of function, fails to oligomerize. 1 Publication | 1 | |
Mutagenesisi | 90 | L → S: Loss of localization to the cell pole. Fails to oligomerize. 2 Publications | 1 | |
Mutagenesisi | 94 | W → R: Loss of localization to the cell pole. 1 Publication | 1 | |
Mutagenesisi | 117 | F → S: Loss of localization to the cell pole. 2 Publications | 1 | |
Mutagenesisi | 143 | D → G: Loss of function. Fails to oligomerize. 1 Publication | 1 | |
Mutagenesisi | 147 | Q → A: Severely diminished function, exclusive clockwise flagellar rotation. 1 Publication | 1 | |
Mutagenesisi | 188 | G → E: Diminished ability to stimulate dephosphorylation of CheY-P. 1 Publication | 1 | |
Mutagenesisi | 205 | V → E: Severely impairs the interaction the interaction with CheY-P. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089641 | 1 – 214 | Protein phosphatase CheZAdd BLAST | 214 |
Proteomic databases
PaxDbi | P0A9H9 |
PRIDEi | P0A9H9 |
2D gel databases
SWISS-2DPAGEi | P0A9H9 |
Interactioni
Subunit structurei
Homodimer.
Interacts with CheA isoform CheA(short).
Interacts (via C-terminus) with phosphorylated CheY (CheY-P).
4 PublicationsBinary interactionsi
Hide detailsP0A9H9
With | #Exp. | IntAct |
---|---|---|
cheA [P07363] | 3 | EBI-546726,EBI-1026773 |
cheY [P0AE67] | 6 | EBI-546726,EBI-546693 |
itself | 2 | EBI-546726,EBI-546726 |
gapA [P0A9B2] | 2 | EBI-546726,EBI-368904 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259554, 392 interactors 850749, 2 interactors |
ComplexPortali | CPX-1088, Chemotaxis phosphorelay complex CheY-CheZ |
DIPi | DIP-9275N |
IntActi | P0A9H9, 22 interactors |
STRINGi | 511145.b1881 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A9H9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A9H9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the CheZ family.Curated
Phylogenomic databases
eggNOGi | COG3143, Bacteria |
HOGENOMi | CLU_080718_1_0_6 |
InParanoidi | P0A9H9 |
PhylomeDBi | P0A9H9 |
Family and domain databases
InterProi | View protein in InterPro IPR007439, Chemotax_Pase_CheZ |
Pfami | View protein in Pfam PF04344, CheZ, 1 hit |
PIRSFi | PIRSF002884, CheZ, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9H9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMQPSIKPAD EHSAGDIIAR IGSLTRMLRD SLRELGLDQA IAEAAEAIPD
60 70 80 90 100
ARDRLYYVVQ MTAQAAERAL NSVEASQPHQ DQMEKSAKAL TQRWDDWFAD
110 120 130 140 150
PIDLADAREL VTDTRQFLAD VPAHTSFTNA QLLEIMMAQD FQDLTGQVIK
160 170 180 190 200
RMMDVIQEIE RQLLMVLLEN IPEQESRPKR ENQSLLNGPQ VDTSKAGVVA
210
SQDQVDDLLD SLGF
Sequence cautioni
The sequence AAA23571 differs from that shown.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 | A → P in AAA23571 (PubMed:3510184).Curated | 1 | |
Sequence conflicti | 130 | A → G in AAA23571 (PubMed:3510184).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13463 Genomic DNA Translation: AAA23571.1 Sequence problems. U00096 Genomic DNA Translation: AAC74951.1 AP009048 Genomic DNA Translation: BAA15697.1 K02175 Genomic DNA Translation: AAA23578.1 |
PIRi | F25195, QRECCZ |
RefSeqi | NP_416395.1, NC_000913.3 WP_000983609.1, NZ_STEB01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC74951; AAC74951; b1881 BAA15697; BAA15697; BAA15697 |
GeneIDi | 49584368 946392 |
KEGGi | ecj:JW1870 eco:b1881 |
PATRICi | fig|511145.12.peg.1962 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13463 Genomic DNA Translation: AAA23571.1 Sequence problems. U00096 Genomic DNA Translation: AAC74951.1 AP009048 Genomic DNA Translation: BAA15697.1 K02175 Genomic DNA Translation: AAA23578.1 |
PIRi | F25195, QRECCZ |
RefSeqi | NP_416395.1, NC_000913.3 WP_000983609.1, NZ_STEB01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KMI | X-ray | 2.90 | Z | 1-214 | [»] | |
SMRi | P0A9H9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259554, 392 interactors 850749, 2 interactors |
ComplexPortali | CPX-1088, Chemotaxis phosphorelay complex CheY-CheZ |
DIPi | DIP-9275N |
IntActi | P0A9H9, 22 interactors |
STRINGi | 511145.b1881 |
2D gel databases
SWISS-2DPAGEi | P0A9H9 |
Proteomic databases
PaxDbi | P0A9H9 |
PRIDEi | P0A9H9 |
Genome annotation databases
EnsemblBacteriai | AAC74951; AAC74951; b1881 BAA15697; BAA15697; BAA15697 |
GeneIDi | 49584368 946392 |
KEGGi | ecj:JW1870 eco:b1881 |
PATRICi | fig|511145.12.peg.1962 |
Organism-specific databases
EchoBASEi | EB0149 |
Phylogenomic databases
eggNOGi | COG3143, Bacteria |
HOGENOMi | CLU_080718_1_0_6 |
InParanoidi | P0A9H9 |
PhylomeDBi | P0A9H9 |
Enzyme and pathway databases
BioCyci | EcoCyc:CHEZ-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A9H9 |
PROi | PR:P0A9H9 |
Family and domain databases
InterProi | View protein in InterPro IPR007439, Chemotax_Pase_CheZ |
Pfami | View protein in Pfam PF04344, CheZ, 1 hit |
PIRSFi | PIRSF002884, CheZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHEZ_ECOLI | |
Accessioni | P0A9H9Primary (citable) accession number: P0A9H9 Secondary accession number(s): P07366 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families