UniProtKB - P0A9H5 (BTUR_ECOLI)
Protein
Corrinoid adenosyltransferase
Gene
btuR
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids (By similarity).By similarity
Catalytic activityi
- 2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H+ + oxidized [electron-transfer flavoprotein] + 2 triphosphateEC:2.5.1.17
- 2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H+ + oxidized [electron-transfer flavoprotein] + 2 triphosphateEC:2.5.1.17
: adenosylcobalamin biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.Proteins known to be involved in the 7 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Corrinoid adenosyltransferase (cobO), Corrinoid adenosyltransferase (cobO), Corrinoid adenosyltransferase (btuR)
- no protein annotated in this organism
- no protein annotated in this organism
- Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
- Bifunctional adenosylcobalamin biosynthesis protein (cobU), Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
- Adenosylcobinamide-GDP ribazoletransferase (cobS), Adenosylcobinamide-GDP ribazoletransferase (cobS)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 42 | ATPBy similarity | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cob(I)yrinic acid a,c-diamide adenosyltransferase activity Source: EcoCyc
GO - Biological processi
- aerobic cobalamin biosynthetic process Source: EcoCyc
- cobalamin biosynthetic process Source: EcoCyc
- porphyrin-containing compound biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Cobalamin biosynthesis, Porphyrin biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:COBALADENOSYLTRANS-MONOMER |
UniPathwayi | UPA00148;UER00233 |
Names & Taxonomyi
Protein namesi | Recommended name: Corrinoid adenosyltransferase (EC:2.5.1.17)Alternative name(s): Cob(II)alamin adenosyltransferase Cob(II)yrinic acid a,c-diamide adenosyltransferase Cobinamide/cobalamin adenosyltransferase |
Gene namesi | Name:btuR Synonyms:cobA Ordered Locus Names:b1270, JW1262 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065009 | 1 – 196 | Corrinoid adenosyltransferaseAdd BLAST | 196 |
Proteomic databases
jPOSTi | P0A9H5 |
PaxDbi | P0A9H5 |
PRIDEi | P0A9H5 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259575, 12 interactors |
IntActi | P0A9H5, 14 interactors |
STRINGi | 511145.b1270 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Cob(I)alamin adenosyltransferase family.Curated
Phylogenomic databases
eggNOGi | COG2109, Bacteria |
HOGENOMi | CLU_088595_0_0_6 |
InParanoidi | P0A9H5 |
PhylomeDBi | P0A9H5 |
Family and domain databases
CDDi | cd00561, CobA_CobO_BtuR, 1 hit |
InterProi | View protein in InterPro IPR003724, CblAdoTrfase_CobA IPR025826, Co_AT_N_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF12557, Co_AT_N, 1 hit PF02572, CobA_CobO_BtuR, 1 hit |
PIRSFi | PIRSF015617, Adensltrnsf_CobA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00708, cobA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9H5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDERYQQRQ QRVKEKVDAR VAQAQDERGI IIVFTGNGKG KTTAAFGTAT
60 70 80 90 100
RAVGHGKKVG VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWDTQNRES
110 120 130 140 150
DTAACREVWQ HAKRMLADSS LDMVLLDELT YMVAYDYLPL EEVVQALNER
160 170 180 190
PHQQTVIITG RGCHRDILEL ADTVSELRPV KHAFDAGVKA QIGIDY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21528 Genomic DNA Translation: AAA23530.1 U00096 Genomic DNA Translation: AAC74352.1 AP009048 Genomic DNA Translation: BAA14807.1 |
PIRi | A64875 |
RefSeqi | NP_415786.1, NC_000913.3 WP_001278906.1, NZ_SSZK01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC74352; AAC74352; b1270 BAA14807; BAA14807; BAA14807 |
GeneIDi | 945839 |
KEGGi | ecj:JW1262 eco:b1270 |
PATRICi | fig|1411691.4.peg.1014 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21528 Genomic DNA Translation: AAA23530.1 U00096 Genomic DNA Translation: AAC74352.1 AP009048 Genomic DNA Translation: BAA14807.1 |
PIRi | A64875 |
RefSeqi | NP_415786.1, NC_000913.3 WP_001278906.1, NZ_SSZK01000031.1 |
3D structure databases
SMRi | P0A9H5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259575, 12 interactors |
IntActi | P0A9H5, 14 interactors |
STRINGi | 511145.b1270 |
Proteomic databases
jPOSTi | P0A9H5 |
PaxDbi | P0A9H5 |
PRIDEi | P0A9H5 |
Genome annotation databases
EnsemblBacteriai | AAC74352; AAC74352; b1270 BAA14807; BAA14807; BAA14807 |
GeneIDi | 945839 |
KEGGi | ecj:JW1262 eco:b1270 |
PATRICi | fig|1411691.4.peg.1014 |
Organism-specific databases
EchoBASEi | EB0128 |
Phylogenomic databases
eggNOGi | COG2109, Bacteria |
HOGENOMi | CLU_088595_0_0_6 |
InParanoidi | P0A9H5 |
PhylomeDBi | P0A9H5 |
Enzyme and pathway databases
UniPathwayi | UPA00148;UER00233 |
BioCyci | EcoCyc:COBALADENOSYLTRANS-MONOMER |
Miscellaneous databases
PROi | PR:P0A9H5 |
Family and domain databases
CDDi | cd00561, CobA_CobO_BtuR, 1 hit |
InterProi | View protein in InterPro IPR003724, CblAdoTrfase_CobA IPR025826, Co_AT_N_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF12557, Co_AT_N, 1 hit PF02572, CobA_CobO_BtuR, 1 hit |
PIRSFi | PIRSF015617, Adensltrnsf_CobA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00708, cobA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BTUR_ECOLI | |
Accessioni | P0A9H5Primary (citable) accession number: P0A9H5 Secondary accession number(s): P13040 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families