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UniProtKB - P0A9G4 (CUER_ECOLI)
Protein
HTH-type transcriptional regulator CueR
Gene
cueR
Organism
Escherichia coli (strain K12)
Status
Functioni
Regulates the transcription of the copA and cueO genes. It detects cytoplasmic copper stress and activates transcription in response to increasing copper concentrations.
3 PublicationsMiscellaneous
CueR tightly binds Cu1+ via a linear S-Cu-S center.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 112 | Copper(1+) | 1 | |
Metal bindingi | 120 | Copper(1+) | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 4 – 23 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: EcoCyc
- copper ion binding Source: EcoCyc
- DNA-binding transcription activator activity Source: CollecTF
- DNA-binding transcription factor activity Source: EcoCyc
- identical protein binding Source: IntAct
- transcription cis-regulatory region binding Source: CollecTF
GO - Biological processi
- positive regulation of transcription, DNA-templated Source: EcoCyc
- transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Copper, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6263-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator CueRAlternative name(s): Copper efflux regulator Copper export regulator |
Gene namesi | Name:cueR1 Publication Synonyms:copR1 Publication, ybbI Ordered Locus Names:b0487, JW0476 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
- protein-DNA complex Source: CollecTF
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Loss of Cu-induction of copA, forms smaller than wild-type colonies when grown on 2 mM CuSO4 (PubMed:11167016).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 112 | C → S: Loss of sensitivity to copper. 2 Publications | 1 | |
Mutagenesisi | 120 | C → S: Loss of sensitivity to copper. 2 Publications | 1 | |
Mutagenesisi | 129 | C → S: No effect on response to copper. 2 Publications | 1 | |
Mutagenesisi | 130 | C → S: No effect on response to copper. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098111 | 1 – 135 | HTH-type transcriptional regulator CueRAdd BLAST | 135 |
Proteomic databases
jPOSTi | P0A9G4 |
PaxDbi | P0A9G4 |
PRIDEi | P0A9G4 |
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
P0A9G4
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-1117686,EBI-1117686 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259848, 11 interactors |
DIPi | DIP-51206N |
IntActi | P0A9G4, 4 interactors |
STRINGi | 511145.b0487 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A9G4 |
SMRi | P0A9G4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A9G4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 69 | HTH merR-typePROSITE-ProRule annotationAdd BLAST | 69 |
Domaini
It contains an N-terminal DNA binding region and a C-terminal metal binding region.
Phylogenomic databases
eggNOGi | COG0789, Bacteria |
HOGENOMi | CLU_060077_2_0_6 |
InParanoidi | P0A9G4 |
OMAi | FNDPERH |
PhylomeDBi | P0A9G4 |
Family and domain databases
CDDi | cd01108, HTH_CueR, 1 hit |
InterProi | View protein in InterPro IPR011789, CueR IPR009061, DNA-bd_dom_put_sf IPR000551, MerR-type_HTH_dom |
Pfami | View protein in Pfam PF13411, MerR_1, 1 hit |
PRINTSi | PR00040, HTHMERR |
SMARTi | View protein in SMART SM00422, HTH_MERR, 1 hit |
SUPFAMi | SSF46955, SSF46955, 1 hit |
TIGRFAMsi | TIGR02044, CueR, 1 hit |
PROSITEi | View protein in PROSITE PS00552, HTH_MERR_1, 1 hit PS50937, HTH_MERR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9G4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNISDVAKIT GLTSKAIRFY EEKGLVTPPM RSENGYRTYT QQHLNELTLL
60 70 80 90 100
RQARQVGFNL EESGELVNLF NDPQRHSADV KRRTLEKVAE IERHIEELQS
110 120 130
MRDQLLALAN ACPGDDSADC PIIENLSGCC HHRAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF318185 Genomic DNA Translation: AAK06655.1 U82664 Genomic DNA Translation: AAB40241.1 U00096 Genomic DNA Translation: AAC73589.1 AP009048 Genomic DNA Translation: BAE76266.1 |
PIRi | F64779 |
RefSeqi | NP_415020.1, NC_000913.3 WP_001026747.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73589; AAC73589; b0487 BAE76266; BAE76266; BAE76266 |
GeneIDi | 66671211 945332 |
KEGGi | ecj:JW0476 eco:b0487 |
PATRICi | fig|1411691.4.peg.1789 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF318185 Genomic DNA Translation: AAK06655.1 U82664 Genomic DNA Translation: AAB40241.1 U00096 Genomic DNA Translation: AAC73589.1 AP009048 Genomic DNA Translation: BAE76266.1 |
PIRi | F64779 |
RefSeqi | NP_415020.1, NC_000913.3 WP_001026747.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q05 | X-ray | 2.20 | A/B | 1-135 | [»] | |
1Q06 | X-ray | 2.07 | A/B | 1-135 | [»] | |
1Q07 | X-ray | 2.50 | A/B | 1-135 | [»] | |
4WLS | X-ray | 2.10 | A/B | 1-128 | [»] | |
4WLW | X-ray | 2.80 | A | 1-135 | [»] | |
6LDI | electron microscopy | 3.69 | G/H | 1-135 | [»] | |
6XH7 | electron microscopy | 3.90 | G/H | 1-135 | [»] | |
6XH8 | electron microscopy | 4.10 | G/H | 1-135 | [»] | |
7C17 | electron microscopy | 4.22 | G/H | 1-135 | [»] | |
AlphaFoldDBi | P0A9G4 | |||||
SMRi | P0A9G4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259848, 11 interactors |
DIPi | DIP-51206N |
IntActi | P0A9G4, 4 interactors |
STRINGi | 511145.b0487 |
Proteomic databases
jPOSTi | P0A9G4 |
PaxDbi | P0A9G4 |
PRIDEi | P0A9G4 |
Genome annotation databases
EnsemblBacteriai | AAC73589; AAC73589; b0487 BAE76266; BAE76266; BAE76266 |
GeneIDi | 66671211 945332 |
KEGGi | ecj:JW0476 eco:b0487 |
PATRICi | fig|1411691.4.peg.1789 |
Organism-specific databases
EchoBASEi | EB3044 |
Phylogenomic databases
eggNOGi | COG0789, Bacteria |
HOGENOMi | CLU_060077_2_0_6 |
InParanoidi | P0A9G4 |
OMAi | FNDPERH |
PhylomeDBi | P0A9G4 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6263-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A9G4 |
PROi | PR:P0A9G4 |
Family and domain databases
CDDi | cd01108, HTH_CueR, 1 hit |
InterProi | View protein in InterPro IPR011789, CueR IPR009061, DNA-bd_dom_put_sf IPR000551, MerR-type_HTH_dom |
Pfami | View protein in Pfam PF13411, MerR_1, 1 hit |
PRINTSi | PR00040, HTHMERR |
SMARTi | View protein in SMART SM00422, HTH_MERR, 1 hit |
SUPFAMi | SSF46955, SSF46955, 1 hit |
TIGRFAMsi | TIGR02044, CueR, 1 hit |
PROSITEi | View protein in PROSITE PS00552, HTH_MERR_1, 1 hit PS50937, HTH_MERR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CUER_ECOLI | |
Accessioni | P0A9G4Primary (citable) accession number: P0A9G4 Secondary accession number(s): P77565, Q2MBU0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references