UniProtKB - P0A9G2 (NHAR_ECOLI)
Protein
Transcriptional activator protein NhaR
Gene
nhaR
Organism
Escherichia coli (strain K12)
Status
Functioni
Plays a role in the positive regulation of NhaA.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 23 – 42 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription activator activity Source: EcoCyc
- DNA-binding transcription factor activity Source: GO_Central
GO - Biological processi
- positive regulation of DNA-templated transcription, initiation Source: EcoCyc
- regulation of DNA-templated transcription, initiation Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00474 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator protein NhaRAlternative name(s): Na(+)/H(+) antiporter regulatory protein |
Gene namesi | Name:nhaR Synonyms:antO Ordered Locus Names:b0020, JW0019 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 134 | E → A in nhaR1; increases the transcription of NhaA. | 1 |
Miscellaneous databases
PHI-basei | PHI:3267 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105689 | 1 – 301 | Transcriptional activator protein NhaRAdd BLAST | 301 |
Proteomic databases
jPOSTi | P0A9G2 |
PaxDbi | P0A9G2 |
PRIDEi | P0A9G2 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259724, 3 interactors |
IntActi | P0A9G2, 2 interactors |
STRINGi | 511145.b0020 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 63 | HTH lysR-typePROSITE-ProRule annotationAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the LysR transcriptional regulatory family.Curated
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_9_0_6 |
InParanoidi | P0A9G2 |
PhylomeDBi | P0A9G2 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9G2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSMSHINYNH LYYFWHVYKE GSVVGAAEAL YLTPQTITGQ IRALEERLQG
60 70 80 90 100
KLFKRKGRGL EPSELGELVY RYADKMFTLS QEMLDIVNYR KESNLLFDVG
110 120 130 140 150
VADALSKRLV SSVLNAAVVE GEPIHLRCFE STHEMLLEQL SQHKLDMIIS
160 170 180 190 200
DCPIDSTQQE GLFSVRIGEC GVSFWCTNPP PEKPFPACLE ERRLLIPGRR
210 220 230 240 250
SMLGRKLLNW FNSQGLNVEI LGEFDDAALM KAFGAMHNAI FVAPTLYAYD
260 270 280 290 300
FYADKTVVEI GRVENVMEEY HAIFAERMIQ HPAVQRICNT DYSALFSPAV
R
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 46 – 47 | ER → DA (PubMed:2429258).Curated | 2 | |
Sequence conflicti | 46 – 47 | ER → DA (Ref. 2) Curated | 2 | |
Sequence conflicti | 46 – 47 | ER → DA (PubMed:1630901).Curated | 2 | |
Sequence conflicti | 50 | G → A (PubMed:2429258).Curated | 1 | |
Sequence conflicti | 50 | G → A (Ref. 2) Curated | 1 | |
Sequence conflicti | 50 | G → A (PubMed:1630901).Curated | 1 | |
Sequence conflicti | 58 – 65 | RGLEPSEL → TWSRTQRA (PubMed:2429258).Curated | 8 | |
Sequence conflicti | 58 – 65 | RGLEPSEL → TWSRTQRA (PubMed:1630901).Curated | 8 | |
Sequence conflicti | 146 | D → E (PubMed:2429258).Curated | 1 | |
Sequence conflicti | 146 | D → E (Ref. 2) Curated | 1 | |
Sequence conflicti | 146 | D → E (PubMed:1630901).Curated | 1 | |
Sequence conflicti | 233 – 301 | FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS (PubMed:2429258).CuratedAdd BLAST | 69 | |
Sequence conflicti | 233 – 301 | FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS (PubMed:1630901).CuratedAdd BLAST | 69 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04382 Genomic DNA Translation: CAA27969.1 L24072 Genomic DNA Translation: AAA24221.1 U00096 Genomic DNA Translation: AAC73131.1 AP009048 Genomic DNA Translation: BAE76032.1 |
RefSeqi | NP_414561.1, NC_000913.3 WP_000062890.1, NZ_CP047127.1 |
Genome annotation databases
EnsemblBacteriai | AAC73131; AAC73131; b0020 BAE76032; BAE76032; BAE76032 |
GeneIDi | 944757 |
KEGGi | ecj:JW0019 eco:b0020 |
PATRICi | fig|511145.12.peg.17 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04382 Genomic DNA Translation: CAA27969.1 L24072 Genomic DNA Translation: AAA24221.1 U00096 Genomic DNA Translation: AAC73131.1 AP009048 Genomic DNA Translation: BAE76032.1 |
RefSeqi | NP_414561.1, NC_000913.3 WP_000062890.1, NZ_CP047127.1 |
3D structure databases
SMRi | P0A9G2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259724, 3 interactors |
IntActi | P0A9G2, 2 interactors |
STRINGi | 511145.b0020 |
Proteomic databases
jPOSTi | P0A9G2 |
PaxDbi | P0A9G2 |
PRIDEi | P0A9G2 |
Genome annotation databases
EnsemblBacteriai | AAC73131; AAC73131; b0020 BAE76032; BAE76032; BAE76032 |
GeneIDi | 944757 |
KEGGi | ecj:JW0019 eco:b0020 |
PATRICi | fig|511145.12.peg.17 |
Organism-specific databases
EchoBASEi | EB1070 |
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_9_0_6 |
InParanoidi | P0A9G2 |
PhylomeDBi | P0A9G2 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00474 |
Miscellaneous databases
PHI-basei | PHI:3267 |
PROi | PR:P0A9G2 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NHAR_ECOLI | |
Accessioni | P0A9G2Primary (citable) accession number: P0A9G2 Secondary accession number(s): P10087 Q83SR2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families