UniProtKB - P0A9F9 (METR_ECOLI)
Protein
HTH-type transcriptional regulator MetR
Gene
metR
Organism
Escherichia coli (strain K12)
Status
Functioni
Control of the last step in methionine biosynthesis; MetR is a positive activator of the metA, metE and metH genes. MetR is also a negative regulator of its own expression. Binds homocysteine as an inducer.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 19 – 38 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- amino acid binding Source: EcoCyc
- DNA binding Source: EcoCyc
- DNA-binding transcription factor activity Source: InterPro
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- methionine biosynthetic process Source: UniProtKB-KW
- regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD03938 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator MetR |
Gene namesi | Name:metR Ordered Locus Names:b3828, JW3804 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105673 | 1 – 317 | HTH-type transcriptional regulator MetRAdd BLAST | 317 |
Proteomic databases
PaxDbi | P0A9F9 |
PRIDEi | P0A9F9 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259476, 2 interactors 852608, 2 interactors |
DIPi | DIP-10198N |
IntActi | P0A9F9, 8 interactors |
STRINGi | 511145.b3828 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A9F9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 59 | HTH lysR-typePROSITE-ProRule annotationAdd BLAST | 59 |
Sequence similaritiesi
Belongs to the LysR transcriptional regulatory family.Curated
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_6_0_6 |
InParanoidi | P0A9F9 |
PhylomeDBi | P0A9F9 |
Family and domain databases
CDDi | cd08441, PBP2_MetR, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR037406, MetR_PBP2 IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9F9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEVKHLKTL QALRNCGSLA AAAATLHQTQ SALSHQFSDL EQRLGFRLFV
60 70 80 90 100
RKSQPLRFTP QGEILLQLAN QVLPQISQAL QACNEPQQTR LRIAIECHSC
110 120 130 140 150
IQWLTPALEN FHKNWPQVEM DFKSGVTFDP QPALQQGELD LVMTSDILPR
160 170 180 190 200
SGLHYSPMFD YEVRLVLAPD HPLAAKTRIT PEDLASETLL IYPVQRSRLD
210 220 230 240 250
VWRHFLQPAG VSPSLKSVDN TLLLIQMVAA RMGIAALPHW VVESFERQGL
260 270 280 290 300
VVTKTLGEGL WSRLYAAVRD GEQRQPVTEA FIRSARNHAC DHLPFVKSAE
310
RPTYDAPTVR PGSPARL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | L → T (PubMed:2643109).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37630 Genomic DNA Translation: AAA62781.1 M87049 Genomic DNA Translation: AAA67624.1 U00096 Genomic DNA Translation: AAC76831.1 AP009048 Genomic DNA Translation: BAE77473.1 J04155 Genomic DNA Translation: AAA24159.2 Sequence problems. |
PIRi | A36066 |
RefSeqi | NP_418272.1, NC_000913.3 WP_000573621.1, NZ_SSZK01000046.1 |
Genome annotation databases
EnsemblBacteriai | AAC76831; AAC76831; b3828 BAE77473; BAE77473; BAE77473 |
GeneIDi | 58389520 948310 |
KEGGi | ecj:JW3804 eco:b3828 |
PATRICi | fig|1411691.4.peg.2880 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37630 Genomic DNA Translation: AAA62781.1 M87049 Genomic DNA Translation: AAA67624.1 U00096 Genomic DNA Translation: AAC76831.1 AP009048 Genomic DNA Translation: BAE77473.1 J04155 Genomic DNA Translation: AAA24159.2 Sequence problems. |
PIRi | A36066 |
RefSeqi | NP_418272.1, NC_000913.3 WP_000573621.1, NZ_SSZK01000046.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5FO5 | X-ray | 2.16 | A | 1-92 | [»] | |
SMRi | P0A9F9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259476, 2 interactors 852608, 2 interactors |
DIPi | DIP-10198N |
IntActi | P0A9F9, 8 interactors |
STRINGi | 511145.b3828 |
Proteomic databases
PaxDbi | P0A9F9 |
PRIDEi | P0A9F9 |
Genome annotation databases
EnsemblBacteriai | AAC76831; AAC76831; b3828 BAE77473; BAE77473; BAE77473 |
GeneIDi | 58389520 948310 |
KEGGi | ecj:JW3804 eco:b3828 |
PATRICi | fig|1411691.4.peg.2880 |
Organism-specific databases
EchoBASEi | EB0586 |
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_6_0_6 |
InParanoidi | P0A9F9 |
PhylomeDBi | P0A9F9 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD03938 |
Miscellaneous databases
PROi | PR:P0A9F9 |
Family and domain databases
CDDi | cd08441, PBP2_MetR, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR037406, MetR_PBP2 IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | METR_ECOLI | |
Accessioni | P0A9F9Primary (citable) accession number: P0A9F9 Secondary accession number(s): P19797, Q2M8D3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families