UniProtKB - P0A9F3 (CYSB_ECOLI)
Protein
HTH-type transcriptional regulator CysB
Gene
cysB
Organism
Escherichia coli (strain K12)
Status
Functioni
This protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB is N-acetylserine. CysB inhibits its own transcription.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 19 – 38 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: EcoCyc
- DNA-binding transcription factor activity Source: InterPro
- identical protein binding Source: EcoCyc
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- cysteine biosynthetic process Source: GO_Central
- regulation of transcription, DNA-templated Source: GO_Central
- response to X-ray Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Amino-acid biosynthesis, Cysteine biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00232 MetaCyc:PD00232 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator CysBAlternative name(s): Cys regulon transcriptional activator |
Gene namesi | Name:cysB Ordered Locus Names:b1275, JW1267 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105613 | 1 – 324 | HTH-type transcriptional regulator CysBAdd BLAST | 324 |
Proteomic databases
jPOSTi | P0A9F3 |
PaxDbi | P0A9F3 |
PRIDEi | P0A9F3 |
Interactioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260138, 28 interactors 850138, 3 interactors |
DIPi | DIP-36048N |
IntActi | P0A9F3, 10 interactors |
STRINGi | 511145.b1275 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 59 | HTH lysR-typePROSITE-ProRule annotationAdd BLAST | 59 |
Sequence similaritiesi
Belongs to the LysR transcriptional regulatory family.Curated
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_6_2_6 |
InParanoidi | P0A9F3 |
PhylomeDBi | P0A9F3 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9F3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFS
60 70 80 90 100
RSGKHLTQVT PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT
110 120 130 140 150
HTQARYALPN VIKGFIERYP RVSLHMHQGS PTQIADAVSK GNADFAIATE
160 170 180 190 200
ALHLYEDLVM LPCYHWNRAI VVTPDHPLAG KKAITIEELA QYPLVTYTFG
210 220 230 240 250
FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV GVIASMAVDP
260 270 280 290 300
VADPDLVRVD AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV
310 320
DAAVALRSNE EIEVMFKDIK LPEK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15041 Genomic DNA Translation: AAA23642.1 M34332 Genomic DNA Translation: AAA23643.1 U00096 Genomic DNA Translation: AAC74357.1 AP009048 Genomic DNA Translation: BAA14827.1 X60293 Genomic DNA Translation: CAA42833.1 |
PIRi | A26695, RGECCB |
RefSeqi | NP_415791.1, NC_000913.3 WP_000776253.1, NZ_STEB01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC74357; AAC74357; b1275 BAA14827; BAA14827; BAA14827 |
GeneIDi | 58460480 945771 |
KEGGi | ecj:JW1267 eco:b1275 |
PATRICi | fig|1411691.4.peg.1009 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15041 Genomic DNA Translation: AAA23642.1 M34332 Genomic DNA Translation: AAA23643.1 U00096 Genomic DNA Translation: AAC74357.1 AP009048 Genomic DNA Translation: BAA14827.1 X60293 Genomic DNA Translation: CAA42833.1 |
PIRi | A26695, RGECCB |
RefSeqi | NP_415791.1, NC_000913.3 WP_000776253.1, NZ_STEB01000005.1 |
3D structure databases
SMRi | P0A9F3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260138, 28 interactors 850138, 3 interactors |
DIPi | DIP-36048N |
IntActi | P0A9F3, 10 interactors |
STRINGi | 511145.b1275 |
Proteomic databases
jPOSTi | P0A9F3 |
PaxDbi | P0A9F3 |
PRIDEi | P0A9F3 |
Genome annotation databases
EnsemblBacteriai | AAC74357; AAC74357; b1275 BAA14827; BAA14827; BAA14827 |
GeneIDi | 58460480 945771 |
KEGGi | ecj:JW1267 eco:b1275 |
PATRICi | fig|1411691.4.peg.1009 |
Organism-specific databases
EchoBASEi | EB0181 |
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_6_2_6 |
InParanoidi | P0A9F3 |
PhylomeDBi | P0A9F3 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00232 MetaCyc:PD00232 |
Miscellaneous databases
PROi | PR:P0A9F3 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSB_ECOLI | |
Accessioni | P0A9F3Primary (citable) accession number: P0A9F3 Secondary accession number(s): P06613, P76834 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families