UniProtKB - P0A9E9 (YEIL_ECOLI)
Protein
Regulatory protein YeiL
Gene
yeiL
Organism
Escherichia coli (strain K12)
Status
Functioni
Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as general nitrogen sources during nitrogen-limited growth.1 Publication
Miscellaneous
Its activity might be modulated by a sensory iron-sulfur cluster, by reversible dithiol-disulfide formation, or by both mechanisms. It is not an oxygen-responsive regulator.
Cofactori
[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 68 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 91 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 93 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 116 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 158 – 181 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12031-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Regulatory protein YeiL |
Gene namesi | Name:yeiL Synonyms:nsr Ordered Locus Names:b2163, JW2150 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication
GO - Cellular componenti
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100182 | 1 – 219 | Regulatory protein YeiLAdd BLAST | 219 |
Proteomic databases
PaxDbi | P0A9E9 |
PRIDEi | P0A9E9 |
Expressioni
Inductioni
In the stationary phase, aerobic expression is positively autoregulated and significantly dependent on RpoS and Lrp. Its transcription is directly or indirectly repressed by FNR.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260462, 27 interactors |
STRINGi | 511145.b2163 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 136 – 199 | HTH crp-typePROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 97 | Sensory domainSequence analysisAdd BLAST | 79 | |
Regioni | 111 – 131 | Dimer interfaceSequence analysisAdd BLAST | 21 |
Phylogenomic databases
eggNOGi | COG0664, Bacteria |
HOGENOMi | CLU_075053_11_1_6 |
PhylomeDBi | P0A9E9 |
Family and domain databases
CDDi | cd00038, CAP_ED, 1 hit |
InterProi | View protein in InterPro IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR012318, HTH_CRP |
Pfami | View protein in Pfam PF00027, cNMP_binding, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 1 hit |
SUPFAMi | SSF51206, SSF51206, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 1 hit PS51063, HTH_CRP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9E9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSESAFKDCF LTDVSADTRL FHFLARDYIV QEGQQPSWLF YLTRGRARLY
60 70 80 90 100
ATLANGRVSL IDFFAAPCFI GEIELIDKDH EPRAVQAIEE CWCLALPMKH
110 120 130 140 150
YRPLLLNDTL FLRKLCVTLS HKNYRNIVSL TQNQSFPLVN RLAAFILLSQ
160 170 180 190 200
EGDLYHEKHT QAAEYLGVSY RHLLYVLAQF IHDGLLIKSK KGYLIKNRKQ
210
LSGLALEMDP ENKFSGMMQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60515.1 U00096 Genomic DNA Translation: AAC75224.1 AP009048 Genomic DNA Translation: BAE76640.1 |
PIRi | B64985 |
RefSeqi | NP_416668.3, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC75224; AAC75224; b2163 BAE76640; BAE76640; BAE76640 |
GeneIDi | 946670 |
KEGGi | ecj:JW2150 eco:b2163 |
PATRICi | fig|511145.12.peg.2248 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60515.1 U00096 Genomic DNA Translation: AAC75224.1 AP009048 Genomic DNA Translation: BAE76640.1 |
PIRi | B64985 |
RefSeqi | NP_416668.3, NC_000913.3 |
3D structure databases
SMRi | P0A9E9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260462, 27 interactors |
STRINGi | 511145.b2163 |
Proteomic databases
PaxDbi | P0A9E9 |
PRIDEi | P0A9E9 |
Genome annotation databases
EnsemblBacteriai | AAC75224; AAC75224; b2163 BAE76640; BAE76640; BAE76640 |
GeneIDi | 946670 |
KEGGi | ecj:JW2150 eco:b2163 |
PATRICi | fig|511145.12.peg.2248 |
Organism-specific databases
EchoBASEi | EB1966 |
Phylogenomic databases
eggNOGi | COG0664, Bacteria |
HOGENOMi | CLU_075053_11_1_6 |
PhylomeDBi | P0A9E9 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12031-MONOMER |
Miscellaneous databases
PROi | PR:P0A9E9 |
Family and domain databases
CDDi | cd00038, CAP_ED, 1 hit |
InterProi | View protein in InterPro IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR012318, HTH_CRP |
Pfami | View protein in Pfam PF00027, cNMP_binding, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 1 hit |
SUPFAMi | SSF51206, SSF51206, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 1 hit PS51063, HTH_CRP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YEIL_ECOLI | |
Accessioni | P0A9E9Primary (citable) accession number: P0A9E9 Secondary accession number(s): P33023, Q2MAR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |