Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase

Gene

dapD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate.1 Publication

Activity regulationi

Inhibited by N-ethylmaleimide, p-chloromercuriphenyl sulfonate, cobalt and copper ions.1 Publication

Kineticsi

  1. KM=15 µM for succinyl-CoA (at pH 7.4)1 Publication
  2. KM=22 µM for tetrahydrodipicolinate (THDPA) (at pH 7.4)1 Publication

    pH dependencei

    Optimum pH is 8.2.1 Publication

    Pathwayi: L-lysine biosynthesis via DAP pathway

    This protein is involved in step 1 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
    2. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
    3. Succinyl-diaminopimelate desuccinylase (dapE)
    This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei104SubstrateBy similarity1
    Binding sitei141SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • diaminopimelate biosynthetic process Source: EcoCyc
    • lysine biosynthetic process Source: EcoCyc
    • lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniRule

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:MONOMER0-2001
    MetaCyc:MONOMER0-2001
    UniPathwayi
    UPA00034;UER00019

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC:2.3.1.117)
    Alternative name(s):
    Tetrahydrodipicolinate N-succinyltransferase
    Short name:
    THDP succinyltransferase
    Short name:
    THP succinyltransferase
    Short name:
    Tetrahydropicolinate succinylase
    Gene namesi
    Name:dapD
    Ordered Locus Names:b0166, JW0161
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10207 dapD

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001969331 – 2742,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferaseAdd BLAST274

    Proteomic databases

    EPDiP0A9D8
    PaxDbiP0A9D8
    PRIDEiP0A9D8

    2D gel databases

    SWISS-2DPAGEiP0A9D8

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    BioGridi4259746, 12 interactors
    DIPiDIP-31866N
    IntActiP0A9D8, 5 interactors
    STRINGi316385.ECDH10B_0145

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9D8
    SMRiP0A9D8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiENOG4105DMJ Bacteria
    COG2171 LUCA
    HOGENOMiHOG000003295
    InParanoidiP0A9D8
    KOiK00674
    OMAiQVPCALI
    PhylomeDBiP0A9D8

    Family and domain databases

    Gene3Di1.10.166.10, 1 hit
    HAMAPiMF_00811 DapD, 1 hit
    InterProiView protein in InterPro
    IPR005664 DapD_Trfase_Hexpep_rpt_fam
    IPR001451 Hexapep
    IPR018357 Hexapep_transf_CS
    IPR023180 THP_succinylTrfase_dom1
    IPR037133 THP_succinylTrfase_N_sf
    IPR011004 Trimer_LpxA-like_sf
    PfamiView protein in Pfam
    PF00132 Hexapep, 1 hit
    PF14602 Hexapep_2, 1 hit
    PF14805 THDPS_N_2, 1 hit
    SUPFAMiSSF51161 SSF51161, 1 hit
    TIGRFAMsiTIGR00965 dapD, 1 hit
    PROSITEiView protein in PROSITE
    PS00101 HEXAPEP_TRANSFERASES, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A9D8-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MQQLQNIIET AFERRAEITP ANADTVTREA VNQVIALLDS GALRVAEKID
    60 70 80 90 100
    GQWVTHQWLK KAVLLSFRIN DNQVIEGAES RYFDKVPMKF ADYDEARFQK
    110 120 130 140 150
    EGFRVVPPAA VRQGAFIARN TVLMPSYVNI GAYVDEGTMV DTWATVGSCA
    160 170 180 190 200
    QIGKNVHLSG GVGIGGVLEP LQANPTIIED NCFIGARSEV VEGVIVEEGS
    210 220 230 240 250
    VISMGVYIGQ STRIYDRETG EIHYGRVPAG SVVVSGNLPS KDGKYSLYCA
    260 270
    VIVKKVDAKT RGKVGINELL RTID
    Length:274
    Mass (Da):29,892
    Last modified:July 19, 2005 - v1
    Checksum:i42D7A38610DD3AF6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti31V → D in AAA23667 (PubMed:6094577).Curated1
    Sequence conflicti163G → R in AAA23667 (PubMed:6094577).Curated1
    Sequence conflicti177I → M in AAA23667 (PubMed:6094577).Curated1
    Sequence conflicti190V → L in AAA23667 (PubMed:6094577).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    K02970 Genomic DNA Translation: AAA23667.1
    U70214 Genomic DNA Translation: AAB08595.1
    U00096 Genomic DNA Translation: AAC73277.1
    AP009048 Genomic DNA Translation: BAB96742.1
    Z21842 Genomic DNA Translation: CAA79888.1
    PIRiF64740 XNECSD
    RefSeqiNP_414708.1, NC_000913.3
    WP_001186650.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73277; AAC73277; b0166
    BAB96742; BAB96742; BAB96742
    GeneIDi944862
    KEGGiecj:JW0161
    eco:b0166
    PATRICifig|1411691.4.peg.2115

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    K02970 Genomic DNA Translation: AAA23667.1
    U70214 Genomic DNA Translation: AAB08595.1
    U00096 Genomic DNA Translation: AAC73277.1
    AP009048 Genomic DNA Translation: BAB96742.1
    Z21842 Genomic DNA Translation: CAA79888.1
    PIRiF64740 XNECSD
    RefSeqiNP_414708.1, NC_000913.3
    WP_001186650.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP0A9D8
    SMRiP0A9D8
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259746, 12 interactors
    DIPiDIP-31866N
    IntActiP0A9D8, 5 interactors
    STRINGi316385.ECDH10B_0145

    2D gel databases

    SWISS-2DPAGEiP0A9D8

    Proteomic databases

    EPDiP0A9D8
    PaxDbiP0A9D8
    PRIDEiP0A9D8

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73277; AAC73277; b0166
    BAB96742; BAB96742; BAB96742
    GeneIDi944862
    KEGGiecj:JW0161
    eco:b0166
    PATRICifig|1411691.4.peg.2115

    Organism-specific databases

    EchoBASEiEB0203
    EcoGeneiEG10207 dapD

    Phylogenomic databases

    eggNOGiENOG4105DMJ Bacteria
    COG2171 LUCA
    HOGENOMiHOG000003295
    InParanoidiP0A9D8
    KOiK00674
    OMAiQVPCALI
    PhylomeDBiP0A9D8

    Enzyme and pathway databases

    UniPathwayi
    UPA00034;UER00019

    BioCyciEcoCyc:MONOMER0-2001
    MetaCyc:MONOMER0-2001

    Miscellaneous databases

    PROiPR:P0A9D8

    Family and domain databases

    Gene3Di1.10.166.10, 1 hit
    HAMAPiMF_00811 DapD, 1 hit
    InterProiView protein in InterPro
    IPR005664 DapD_Trfase_Hexpep_rpt_fam
    IPR001451 Hexapep
    IPR018357 Hexapep_transf_CS
    IPR023180 THP_succinylTrfase_dom1
    IPR037133 THP_succinylTrfase_N_sf
    IPR011004 Trimer_LpxA-like_sf
    PfamiView protein in Pfam
    PF00132 Hexapep, 1 hit
    PF14602 Hexapep_2, 1 hit
    PF14805 THDPS_N_2, 1 hit
    SUPFAMiSSF51161 SSF51161, 1 hit
    TIGRFAMsiTIGR00965 dapD, 1 hit
    PROSITEiView protein in PROSITE
    PS00101 HEXAPEP_TRANSFERASES, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDAPD_ECOLI
    AccessioniPrimary (citable) accession number: P0A9D8
    Secondary accession number(s): P03948
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 1986
    Last sequence update: July 19, 2005
    Last modified: October 10, 2018
    This is version 109 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again