UniProtKB - P0A9C5 (GLN1B_ECOLI)
Protein
Glutamine synthetase
Gene
glnA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity
Catalytic activityi
- EC:6.3.1.2By similarity
Cofactori
Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity
Activity regulationi
The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 130 | Magnesium 1By similarity | 1 | |
Metal bindingi | 132 | Magnesium 2By similarity | 1 | |
Binding sitei | 208 | ATPBy similarity | 1 | |
Metal bindingi | 213 | Magnesium 2By similarity | 1 | |
Metal bindingi | 221 | Magnesium 2By similarity | 1 | |
Binding sitei | 266 | L-glutamate; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 270 | Magnesium 1; via pros nitrogenBy similarity | 1 | |
Binding sitei | 274 | ATPBy similarity | 1 | |
Binding sitei | 322 | L-glutamateBy similarity | 1 | |
Binding sitei | 328 | L-glutamateBy similarity | 1 | |
Binding sitei | 340 | ATPBy similarity | 1 | |
Binding sitei | 340 | L-glutamateBy similarity | 1 | |
Binding sitei | 345 | ATPBy similarity | 1 | |
Binding sitei | 353 | ATPBy similarity | 1 | |
Metal bindingi | 358 | Magnesium 1By similarity | 1 | |
Binding sitei | 360 | L-glutamateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 272 – 274 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-ammonia ligase activity Source: EcoCyc
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- ammonia assimilation cycle Source: EcoCyc
- glutamine biosynthetic process Source: GO_Central
- nitrogen utilization Source: EcoCyc
- response to radiation Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLUTAMINESYN-MONOMER MetaCyc:GLUTAMINESYN-MONOMER |
SABIO-RKi | P0A9C5 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)Short name: GSBy similarity Alternative name(s): Glutamate--ammonia ligaseCurated Glutamine synthetase I betaBy similarity Short name: GSI betaBy similarity |
Gene namesi | Name:glnABy similarity Ordered Locus Names:b3870, JW3841 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000153235 | 2 – 469 | Glutamine synthetaseAdd BLAST | 468 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 398 | O-AMP-tyrosine2 Publications | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0A9C5 |
PaxDbi | P0A9C5 |
PRIDEi | P0A9C5 |
2D gel databases
SWISS-2DPAGEi | P0A9C5 |
PTM databases
MetOSitei | P0A9C5 |
Interactioni
Subunit structurei
Oligomer of 12 subunits arranged in the form of two hexameric ring.
By similarityBinary interactionsi
P0A9C5
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-909063,EBI-909063 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262630, 46 interactors 852667, 1 interactor |
DIPi | DIP-9777N |
IntActi | P0A9C5, 10 interactors |
STRINGi | 511145.b3870 |
Chemistry databases
BindingDBi | P0A9C5 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 265 – 266 | L-glutamate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the glutamine synthetase family.Curated
Phylogenomic databases
eggNOGi | COG0174, Bacteria |
HOGENOMi | CLU_017290_1_2_6 |
InParanoidi | P0A9C5 |
PhylomeDBi | P0A9C5 |
Family and domain databases
Gene3Di | 3.10.20.70, 1 hit |
InterProi | View protein in InterPro IPR008147, Gln_synt_b-grasp IPR036651, Gln_synt_N IPR014746, Gln_synth/guanido_kin_cat_dom IPR008146, Gln_synth_cat_dom IPR027303, Gln_synth_gly_rich_site IPR004809, Gln_synth_I IPR001637, Gln_synth_I_adenylation_site IPR027302, Gln_synth_N_conserv_site |
Pfami | View protein in Pfam PF00120, Gln-synt_C, 1 hit PF03951, Gln-synt_N, 1 hit |
SMARTi | View protein in SMART SM01230, Gln-synt_C, 1 hit |
SUPFAMi | SSF54368, SSF54368, 1 hit SSF55931, SSF55931, 1 hit |
TIGRFAMsi | TIGR00653, GlnA, 1 hit |
PROSITEi | View protein in PROSITE PS00180, GLNA_1, 1 hit PS00182, GLNA_ADENYLATION, 1 hit PS00181, GLNA_ATP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A9C5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAEHVLTML NEHEVKFVDL RFTDTKGKEQ HVTIPAHQVN AEFFEEGKMF
60 70 80 90 100
DGSSIGGWKG INESDMVLMP DASTAVIDPF FADSTLIIRC DILEPGTLQG
110 120 130 140 150
YDRDPRSIAK RAEDYLRSTG IADTVLFGPE PEFFLFDDIR FGSSISGSHV
160 170 180 190 200
AIDDIEGAWN SSTQYEGGNK GHRPAVKGGY FPVPPVDSAQ DIRSEMCLVM
210 220 230 240 250
EQMGLVVEAH HHEVATAGQN EVATRFNTMT KKADEIQIYK YVVHNVAHRF
260 270 280 290 300
GKTATFMPKP MFGDNGSGMH CHMSLSKNGV NLFAGDKYAG LSEQALYYIG
310 320 330 340 350
GVIKHAKAIN ALANPTTNSY KRLVPGYEAP VMLAYSARNR SASIRIPVVS
360 370 380 390 400
SPKARRIEVR FPDPAANPYL CFAALLMAGL DGIKNKIHPG EAMDKNLYDL
410 420 430 440 450
PPEEAKEIPQ VAGSLEEALN ELDLDREFLK AGGVFTDEAI DAYIALRREE
460
DDRVRMTPHP VEFELYYSV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 90 | C → S in AAA23879 (PubMed:2874141).Curated | 1 | |
Sequence conflicti | 108 – 109 | IA → MS in AAA23879 (PubMed:2874141).Curated | 2 | |
Sequence conflicti | 197 | C → S in AAA23879 (PubMed:2874141).Curated | 1 | |
Sequence conflicti | 228 | T → I in CAA28806 (PubMed:2882477).Curated | 1 | |
Sequence conflicti | 247 – 248 | AH → VRN in CAA28806 (PubMed:2882477).Curated | 2 | |
Sequence conflicti | 305 – 306 | HA → QP in CAA28806 (PubMed:2882477).Curated | 2 | |
Sequence conflicti | 394 – 395 | DK → KD AA sequence (PubMed:5543675).Curated | 2 | |
Sequence conflicti | 394 – 395 | DK → KD AA sequence (PubMed:4904088).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05173 Genomic DNA Translation: CAA28806.1 M13746 Genomic DNA Translation: AAA23879.1 L19201 Genomic DNA Translation: AAB03004.1 U00096 Genomic DNA Translation: AAC76867.1 AP009048 Genomic DNA Translation: BAE77439.1 J01618 Genomic DNA Translation: AAA98066.1 M10421 Genomic DNA Translation: AAA23882.1 K02176 Genomic DNA Translation: AAA23880.1 |
PIRi | S40815, AJECQ |
RefSeqi | NP_418306.1, NC_000913.3 WP_001271717.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76867; AAC76867; b3870 BAE77439; BAE77439; BAE77439 |
GeneIDi | 58390862 948370 |
KEGGi | ecj:JW3841 eco:b3870 |
PATRICi | fig|1411691.4.peg.2841 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05173 Genomic DNA Translation: CAA28806.1 M13746 Genomic DNA Translation: AAA23879.1 L19201 Genomic DNA Translation: AAB03004.1 U00096 Genomic DNA Translation: AAC76867.1 AP009048 Genomic DNA Translation: BAE77439.1 J01618 Genomic DNA Translation: AAA98066.1 M10421 Genomic DNA Translation: AAA23882.1 K02176 Genomic DNA Translation: AAA23880.1 |
PIRi | S40815, AJECQ |
RefSeqi | NP_418306.1, NC_000913.3 WP_001271717.1, NZ_STEB01000017.1 |
3D structure databases
SMRi | P0A9C5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262630, 46 interactors 852667, 1 interactor |
DIPi | DIP-9777N |
IntActi | P0A9C5, 10 interactors |
STRINGi | 511145.b3870 |
Chemistry databases
BindingDBi | P0A9C5 |
ChEMBLi | CHEMBL3789 |
PTM databases
MetOSitei | P0A9C5 |
2D gel databases
SWISS-2DPAGEi | P0A9C5 |
Proteomic databases
jPOSTi | P0A9C5 |
PaxDbi | P0A9C5 |
PRIDEi | P0A9C5 |
Genome annotation databases
EnsemblBacteriai | AAC76867; AAC76867; b3870 BAE77439; BAE77439; BAE77439 |
GeneIDi | 58390862 948370 |
KEGGi | ecj:JW3841 eco:b3870 |
PATRICi | fig|1411691.4.peg.2841 |
Organism-specific databases
EchoBASEi | EB0378 |
Phylogenomic databases
eggNOGi | COG0174, Bacteria |
HOGENOMi | CLU_017290_1_2_6 |
InParanoidi | P0A9C5 |
PhylomeDBi | P0A9C5 |
Enzyme and pathway databases
BioCyci | EcoCyc:GLUTAMINESYN-MONOMER MetaCyc:GLUTAMINESYN-MONOMER |
SABIO-RKi | P0A9C5 |
Miscellaneous databases
PROi | PR:P0A9C5 |
Family and domain databases
Gene3Di | 3.10.20.70, 1 hit |
InterProi | View protein in InterPro IPR008147, Gln_synt_b-grasp IPR036651, Gln_synt_N IPR014746, Gln_synth/guanido_kin_cat_dom IPR008146, Gln_synth_cat_dom IPR027303, Gln_synth_gly_rich_site IPR004809, Gln_synth_I IPR001637, Gln_synth_I_adenylation_site IPR027302, Gln_synth_N_conserv_site |
Pfami | View protein in Pfam PF00120, Gln-synt_C, 1 hit PF03951, Gln-synt_N, 1 hit |
SMARTi | View protein in SMART SM01230, Gln-synt_C, 1 hit |
SUPFAMi | SSF54368, SSF54368, 1 hit SSF55931, SSF55931, 1 hit |
TIGRFAMsi | TIGR00653, GlnA, 1 hit |
PROSITEi | View protein in PROSITE PS00180, GLNA_1, 1 hit PS00182, GLNA_ADENYLATION, 1 hit PS00181, GLNA_ATP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLN1B_ECOLI | |
Accessioni | P0A9C5Primary (citable) accession number: P0A9C5 Secondary accession number(s): P06711, Q2M8G7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 129 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families