UniProtKB - P0A9C0 (GLPA_ECOLI)
Protein
Anaerobic glycerol-3-phosphate dehydrogenase subunit A
Gene
glpA
Organism
Escherichia coli (strain K12)
Status
Functioni
Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.
Catalytic activityi
- EC:1.1.5.3
Cofactori
Protein has several cofactor binding sites:: glycerol degradation via glycerol kinase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).Proteins known to be involved in this subpathway in this organism are:
- Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB), Anaerobic glycerol-3-phosphate dehydrogenase subunit C (glpC), Anaerobic glycerol-3-phosphate dehydrogenase subunit A (glpA), Glycerol-3-phosphate dehydrogenase (glpA), Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 38 | FADSequence analysisAdd BLAST | 29 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: EcoCyc
- FMN binding Source: InterPro
- glycerol-3-phosphate dehydrogenase (quinone) activity Source: EcoCyc
- sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- glycerol-3-phosphate catabolic process Source: EcoCyc
- glycerol catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | EcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER |
UniPathwayi | UPA00618;UER00673 |
Names & Taxonomyi
Protein namesi | Recommended name: Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC:1.1.5.3)Short name: G-3-P dehydrogenase |
Gene namesi | Name:glpA Ordered Locus Names:b2241, JW2235 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane ; Peripheral membrane protein Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.
GO - Cellular componenti
- cytosol Source: EcoCyc
- glycerol-3-phosphate dehydrogenase complex Source: InterPro
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126093 | 1 – 542 | Anaerobic glycerol-3-phosphate dehydrogenase subunit AAdd BLAST | 542 |
Proteomic databases
jPOSTi | P0A9C0 |
PaxDbi | P0A9C0 |
PRIDEi | P0A9C0 |
Interactioni
Subunit structurei
Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.
Protein-protein interaction databases
BioGRIDi | 4259614, 348 interactors |
ComplexPortali | CPX-4841, Anaerobic glycerol-3-phosphate dehydrogenase complex |
IntActi | P0A9C0, 4 interactors |
STRINGi | 511145.b2241 |
Family & Domainsi
Sequence similaritiesi
Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG0578, Bacteria |
HOGENOMi | CLU_015740_0_1_6 |
InParanoidi | P0A9C0 |
PhylomeDBi | P0A9C0 |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR000447, G3P_DH_FAD-dep IPR017752, G3P_DH_GlpA_su |
PANTHERi | PTHR11985, PTHR11985, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF04324, Fer2_BFD, 1 hit |
PRINTSi | PR01001, FADG3PDH |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03377, glycerol3P_GlpA, 1 hit |
PROSITEi | View protein in PROSITE PS00977, FAD_G3PDH_1, 1 hit PS00978, FAD_G3PDH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A9C0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTRDSQSSD VIIIGGGATG AGIARDCALR GLRVILVERH DIATGATGRN
60 70 80 90 100
HGLLHSGARY AVTDAESARE CISENQILKR IARHCVEPTN GLFITLPEDD
110 120 130 140 150
LSFQATFIRA CEEAGISAEA IDPQQARIIE PAVNPALIGA VKVPDGTVDP
160 170 180 190 200
FRLTAANMLD AKEHGAVILT AHEVTGLIRE GATVCGVRVR NHLTGETQAL
210 220 230 240 250
HAPVVVNAAG IWGQHIAEYA DLRIRMFPAK GSLLIMDHRI NQHVINRCRK
260 270 280 290 300
PSDADILVPG DTISLIGTTS LRIDYNEIDD NRVTAEEVDI LLREGEKLAP
310 320 330 340 350
VMAKTRILRA YSGVRPLVAS DDDPSGRNVS RGIVLLDHAE RDGLDGFITI
360 370 380 390 400
TGGKLMTYRL MAEWATDAVC RKLGNTRPCT TADLALPGSQ EPAEVTLRKV
410 420 430 440 450
ISLPAPLRGS AVYRHGDRTP AWLSEGRLHR SLVCECEAVT AGEVQYAVEN
460 470 480 490 500
LNVNSLLDLR RRTRVGMGTC QGELCACRAA GLLQRFNVTT SAQSIEQLST
510 520 530 540
FLNERWKGVQ PIAWGDALRE SEFTRWVYQG LCGLEKEQKD AL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 329 | V → L in AAA83864 (PubMed:3286606).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20938 Genomic DNA Translation: AAA83864.1 U00096 Genomic DNA Translation: AAC75301.1 AP009048 Genomic DNA Translation: BAA16060.1 |
PIRi | A32006, DEECNA |
RefSeqi | NP_416744.1, NC_000913.3 WP_000857257.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75301; AAC75301; b2241 BAA16060; BAA16060; BAA16060 |
GeneIDi | 48134920 946713 |
KEGGi | ecj:JW2235 eco:b2241 |
PATRICi | fig|1411691.4.peg.4498 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20938 Genomic DNA Translation: AAA83864.1 U00096 Genomic DNA Translation: AAC75301.1 AP009048 Genomic DNA Translation: BAA16060.1 |
PIRi | A32006, DEECNA |
RefSeqi | NP_416744.1, NC_000913.3 WP_000857257.1, NZ_LN832404.1 |
3D structure databases
SMRi | P0A9C0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259614, 348 interactors |
ComplexPortali | CPX-4841, Anaerobic glycerol-3-phosphate dehydrogenase complex |
IntActi | P0A9C0, 4 interactors |
STRINGi | 511145.b2241 |
Proteomic databases
jPOSTi | P0A9C0 |
PaxDbi | P0A9C0 |
PRIDEi | P0A9C0 |
Genome annotation databases
EnsemblBacteriai | AAC75301; AAC75301; b2241 BAA16060; BAA16060; BAA16060 |
GeneIDi | 48134920 946713 |
KEGGi | ecj:JW2235 eco:b2241 |
PATRICi | fig|1411691.4.peg.4498 |
Organism-specific databases
EchoBASEi | EB0386 |
Phylogenomic databases
eggNOGi | COG0578, Bacteria |
HOGENOMi | CLU_015740_0_1_6 |
InParanoidi | P0A9C0 |
PhylomeDBi | P0A9C0 |
Enzyme and pathway databases
UniPathwayi | UPA00618;UER00673 |
BioCyci | EcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER |
Miscellaneous databases
PROi | PR:P0A9C0 |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR000447, G3P_DH_FAD-dep IPR017752, G3P_DH_GlpA_su |
PANTHERi | PTHR11985, PTHR11985, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF04324, Fer2_BFD, 1 hit |
PRINTSi | PR01001, FADG3PDH |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03377, glycerol3P_GlpA, 1 hit |
PROSITEi | View protein in PROSITE PS00977, FAD_G3PDH_1, 1 hit PS00978, FAD_G3PDH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLPA_ECOLI | |
Accessioni | P0A9C0Primary (citable) accession number: P0A9C0 Secondary accession number(s): P13032, P78238 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families