UniProtKB - P0A968 (CSPD_ECOLI)
Protein
Cold shock-like protein CspD
Gene
cspD
Organism
Escherichia coli (strain K12)
Status
Functioni
Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single-stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells.
Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Overproduction is toxic.
Miscellaneous
Binds single-stranded DNA and RNA, but not double-stranded DNA, through hydrophobic interactions without sequence specificity, resulting in a packed structure.
GO - Molecular functioni
- nucleic acid binding Source: GO_Central
- RNA binding Source: EcoCyc
- single-stranded DNA binding Source: EcoCyc
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- negative regulation of DNA replication Source: EcoCyc
- regulation of gene expression Source: GO_Central
- regulation of transcription, DNA-templated Source: InterPro
- response to starvation Source: EcoliWiki
Keywordsi
Molecular function | DNA replication inhibitor, DNA-binding, RNA-binding, Toxin |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11111-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cold shock-like protein CspDShort name: CSP-D |
Gene namesi | Name:cspD Synonyms:cspH, ybjA Ordered Locus Names:b0880, JW0864 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Deletion represses the production of persister cells.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100248 | 1 – 74 | Cold shock-like protein CspDAdd BLAST | 74 |
Proteomic databases
jPOSTi | P0A968 |
PaxDbi | P0A968 |
PRIDEi | P0A968 |
Expressioni
Inductioni
Not induced by cold-shock. Stationary-phase and starvation inducible, as well as by oxidative stress (30 mM H2O2). Repressed by MqsA and MqsRA toxin-antitoxin system.1 Publication
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
P0A968
With | #Exp. | IntAct |
---|---|---|
dppD [P0AAG0] | 3 | EBI-547937,EBI-548206 |
Protein-protein interaction databases
BioGRIDi | 4263137, 624 interactors 850041, 1 interactor |
DIPi | DIP-47833N |
IntActi | P0A968, 39 interactors |
STRINGi | 511145.b0880 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 64 | CSDAdd BLAST | 61 |
Phylogenomic databases
eggNOGi | COG1278, Bacteria |
HOGENOMi | CLU_117621_0_2_6 |
InParanoidi | P0A968 |
PhylomeDBi | P0A968 |
Family and domain databases
CDDi | cd04458, CSP_CDS, 1 hit |
DisProti | DP02900 |
InterProi | View protein in InterPro IPR012156, Cold_shock_CspA IPR011129, CSD IPR019844, CSD_1 IPR002059, CSP_DNA-bd IPR012751, CspD IPR012340, NA-bd_OB-fold |
Pfami | View protein in Pfam PF00313, CSD, 1 hit |
PIRSFi | PIRSF002599, Cold_shock_A, 1 hit |
PRINTSi | PR00050, COLDSHOCK |
SMARTi | View protein in SMART SM00357, CSP, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR02381, cspD, 1 hit |
PROSITEi | View protein in PROSITE PS00352, CSD_1, 1 hit PS51857, CSD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A968-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKGTVKWFN NAKGFGFICP EGGGEDIFAH YSTIQMDGYR TLKAGQSVQF
60 70
DVHQGPKGNH ASVIVPVEVE AAVA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | C → E AA sequence (PubMed:9868784).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31045 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73967.1 AP009048 Genomic DNA Translation: BAA35599.1 |
PIRi | H64826 |
RefSeqi | NP_415401.1, NC_000913.3 WP_000410785.1, NZ_STEB01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC73967; AAC73967; b0880 BAA35599; BAA35599; BAA35599 |
GeneIDi | 58462760 945669 |
KEGGi | ecj:JW0864 eco:b0880 |
PATRICi | fig|1411691.4.peg.1397 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31045 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73967.1 AP009048 Genomic DNA Translation: BAA35599.1 |
PIRi | H64826 |
RefSeqi | NP_415401.1, NC_000913.3 WP_000410785.1, NZ_STEB01000006.1 |
3D structure databases
SMRi | P0A968 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263137, 624 interactors 850041, 1 interactor |
DIPi | DIP-47833N |
IntActi | P0A968, 39 interactors |
STRINGi | 511145.b0880 |
Proteomic databases
jPOSTi | P0A968 |
PaxDbi | P0A968 |
PRIDEi | P0A968 |
Genome annotation databases
EnsemblBacteriai | AAC73967; AAC73967; b0880 BAA35599; BAA35599; BAA35599 |
GeneIDi | 58462760 945669 |
KEGGi | ecj:JW0864 eco:b0880 |
PATRICi | fig|1411691.4.peg.1397 |
Organism-specific databases
EchoBASEi | EB1102 |
Phylogenomic databases
eggNOGi | COG1278, Bacteria |
HOGENOMi | CLU_117621_0_2_6 |
InParanoidi | P0A968 |
PhylomeDBi | P0A968 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11111-MONOMER |
Miscellaneous databases
PROi | PR:P0A968 |
Family and domain databases
CDDi | cd04458, CSP_CDS, 1 hit |
DisProti | DP02900 |
InterProi | View protein in InterPro IPR012156, Cold_shock_CspA IPR011129, CSD IPR019844, CSD_1 IPR002059, CSP_DNA-bd IPR012751, CspD IPR012340, NA-bd_OB-fold |
Pfami | View protein in Pfam PF00313, CSD, 1 hit |
PIRSFi | PIRSF002599, Cold_shock_A, 1 hit |
PRINTSi | PR00050, COLDSHOCK |
SMARTi | View protein in SMART SM00357, CSP, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR02381, cspD, 1 hit |
PROSITEi | View protein in PROSITE PS00352, CSD_1, 1 hit PS51857, CSD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CSPD_ECOLI | |
Accessioni | P0A968Primary (citable) accession number: P0A968 Secondary accession number(s): P24245 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | April 7, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |