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Protein

L-asparaginase 1

Gene

ansA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

E.coli contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.

Catalytic activityi

L-asparagine + H2O = L-aspartate + NH3.1 Publication

Activity regulationi

Shows cooperative activation. Allosterically activated by asparagine.1 Publication

Kineticsi

  1. KM=3.5 mM for L-asparagine

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei14O-isoaspartyl threonine intermediatePROSITE-ProRule annotation1 Publication1
    Binding sitei162Allosteric activator1
    Binding sitei240Allosteric activator1

    GO - Molecular functioni

    • asparaginase activity Source: EcoCyc
    • identical protein binding Source: EcoCyc

    GO - Biological processi

    • asparagine catabolic process via L-aspartate Source: EcoCyc

    Keywordsi

    Molecular functionAllosteric enzyme, Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:ANSA-MONOMER
    MetaCyc:ANSA-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-asparaginase 1 (EC:3.5.1.1)
    Alternative name(s):
    L-asparaginase I
    Short name:
    L-ASNase I
    L-asparagine amidohydrolase I
    Gene namesi
    Name:ansA
    Ordered Locus Names:b1767, JW1756
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10045 ansA

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi14T → A or V: Loss of enzyme activity. 1 Publication1
    Mutagenesisi61S → Q: Loss of enzyme activity. 1
    Mutagenesisi91T → A or V: Loss of enzyme activity. 1 Publication1
    Mutagenesisi118Q → D: Loss of enzyme activity. 1 Publication1
    Mutagenesisi162T → A: No effect on activity at saturating substrate concentration. Abolishes cooperativity. 1 Publication1
    Mutagenesisi240R → A: No effect on activity at saturating substrate concentration. Reduced activity at lower substrate concentrations. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001710791 – 338L-asparaginase 1Add BLAST338

    Proteomic databases

    PaxDbiP0A962
    PRIDEiP0A962

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi4259141, 16 interactors
    850636, 1 interactor
    DIPiDIP-9109N
    IntActiP0A962, 6 interactors
    STRINGi316385.ECDH10B_1905

    Structurei

    Secondary structure

    1338
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP0A962
    SMRiP0A962
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A962

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini4 – 329Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST326

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni59 – 61Substrate binding3
    Regioni91 – 92Substrate binding2
    Regioni271 – 273Allosteric activator binding3

    Sequence similaritiesi

    Belongs to the asparaginase 1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105HDK Bacteria
    COG0252 LUCA
    HOGENOMiHOG000227974
    InParanoidiP0A962
    KOiK01424
    OMAiAIVPKLM
    PhylomeDBiP0A962

    Family and domain databases

    Gene3Di3.40.50.1170, 1 hit
    3.40.50.40, 1 hit
    InterProiView protein in InterPro
    IPR006033 AsnASEI
    IPR036152 Asp/glu_Ase-like_sf
    IPR006034 Asparaginase/glutaminase-like
    IPR020827 Asparaginase/glutaminase_AS1
    IPR027475 Asparaginase/glutaminase_AS2
    IPR027473 L-asparaginase_C
    IPR027474 L-asparaginase_N
    IPR037152 L-asparaginase_N_sf
    PfamiView protein in Pfam
    PF00710 Asparaginase, 1 hit
    PIRSFiPIRSF001220 L-ASNase_gatD, 1 hit
    PRINTSiPR00139 ASNGLNASE
    SMARTiView protein in SMART
    SM00870 Asparaginase, 1 hit
    SUPFAMiSSF53774 SSF53774, 1 hit
    TIGRFAMsiTIGR00519 asnASE_I, 1 hit
    PROSITEiView protein in PROSITE
    PS00144 ASN_GLN_ASE_1, 1 hit
    PS00917 ASN_GLN_ASE_2, 1 hit
    PS51732 ASN_GLN_ASE_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A962-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT
    60 70 80 90 100
    IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA
    110 120 130 140 150
    LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF
    160 170 180 190 200
    FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG
    210 220 230 240 250
    ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN
    260 270 280 290 300
    KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM
    310 320 330
    TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD
    Length:338
    Mass (Da):37,127
    Last modified:July 19, 2005 - v1
    Checksum:i75D97E6D10E9F8DA
    GO

    Sequence cautioni

    The sequence AAA23446 differs from that shown. Reason: Erroneous initiation.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M26934 Genomic DNA Translation: AAA23446.1 Different initiation.
    U00096 Genomic DNA Translation: AAC74837.1
    AP009048 Genomic DNA Translation: BAA15558.1
    PIRiG64936 XDEC1
    RefSeqiNP_416281.1, NC_000913.3
    WP_001170162.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74837; AAC74837; b1767
    BAA15558; BAA15558; BAA15558
    GeneIDi946278
    KEGGiecj:JW1756
    eco:b1767
    PATRICifig|1411691.4.peg.487

    Similar proteinsi

    Entry informationi

    Entry nameiASPG1_ECOLI
    AccessioniPrimary (citable) accession number: P0A962
    Secondary accession number(s): P18840
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: September 12, 2018
    This is version 104 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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