UniProtKB - P0A955 (ALKH_ECOLI)
Protein
KHG/KDPG aldolase
Gene
eda
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.2 Publications
Catalytic activityi
Kineticsi
Kcat is 80 sec(-1) for KDPG, 0.012 sec(-1) for pyruvate and 0.0004 sec(-1) for 2-oxobutyrate.
- KM=0.1 mM for KDPG2 Publications
- KM=9 mM for 2-oxobutyrate2 Publications
- KM=10 mM for pyruvate2 Publications
: 2-dehydro-3-deoxy-D-gluconate degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- 2-dehydro-3-deoxygluconokinase (kdgK)
- 2-dehydro-3-deoxy-phosphogluconate aldolase (FAZ83_02035), KHG/KDPG aldolase (eda)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.
Pathwayi: glyoxylate and dicarboxylate metabolism
This protein is involved in the pathway glyoxylate and dicarboxylate metabolism, which is part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the pathway glyoxylate and dicarboxylate metabolism and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 45 | Proton acceptor | 1 | |
Active sitei | 49 | 1 | ||
Binding sitei | 49 | Substrate1 Publication | 1 | |
Binding sitei | 73 | Substrate1 Publication | 1 | |
Active sitei | 133 | Schiff-base intermediate with substrate | 1 | |
Binding sitei | 133 | Substrate; covalent1 Publication | 1 | |
Binding sitei | 161 | Substrate1 Publication | 1 | |
Sitei | 161 | Plays a major role in determining the stereoselectivity | 1 | |
Binding sitei | 163 | Substrate; via amide nitrogen1 Publication | 1 | |
Binding sitei | 184 | Substrate1 Publication | 1 |
GO - Molecular functioni
- (4S)-4-hydroxy-2-oxoglutarate aldolase activity Source: EcoCyc
- 2-dehydro-3-deoxy-phosphogluconate aldolase activity Source: EcoCyc
- 4-hydroxy-2-oxoglutarate aldolase activity Source: UniProtKB
- identical protein binding Source: IntAct
- oxaloacetate decarboxylase activity Source: EcoCyc
- oxo-acid-lyase activity Source: EcoCyc
GO - Biological processi
- metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase, Multifunctional enzyme |
Ligand | Schiff base |
Enzyme and pathway databases
BioCyci | EcoCyc:KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER MetaCyc:KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER |
BRENDAi | 4.1.2.14, 2026 4.1.3.42, 2026 |
UniPathwayi | UPA00227 UPA00856;UER00829 |
Names & Taxonomyi
Protein namesi | Recommended name: KHG/KDPG aldolaseIncluding the following 2 domains: 4-hydroxy-2-oxoglutarate aldolase (EC:4.1.3.16) Alternative name(s): 2-keto-4-hydroxyglutarate aldolase Short name: KHG-aldolase 2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14) Alternative name(s): 2-keto-3-deoxy-6-phosphogluconate aldolase Short name: KDPG-aldolase Phospho-2-dehydro-3-deoxygluconate aldolase Phospho-2-keto-3-deoxygluconate aldolase |
Gene namesi | Name:eda Synonyms:hga, kdgA Ordered Locus Names:b1850, JW1839 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene lose KHG aldolase activity.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 45 | E → N: 50-fold decrease in catalytic efficiency and 6-fold decrease of binding affinity. 2 Publications | 1 | |
Mutagenesisi | 133 | K → Q: Absence of aldolase activity. Shows a markedly altered substrate specificity relative to the wild-type, with an enhanced activity against pyridine carboxaldehyde, benzaldehyde, and alpha-ketobutyrate; when associated with k-161. 1 Publication | 1 | |
Mutagenesisi | 161 | T → K: Shows activity significantly greater than wild-type. Shows a markedly altered substrate specificity relative to the wild-type, with an enhanced activity against pyridine carboxaldehyde, benzaldehyde, and alpha-ketobutyrate; when associated with Q-133. 2 Publications | 1 | |
Mutagenesisi | 161 | T → V: Little stereoselectivity, accepting KDPG and KDPGal as substrate with roughly equal efficacy. It strongly diminishes the activity against KDPG and slightly increases activity against KDPGal. 2 Publications | 1 | |
Mutagenesisi | 168 | N → S: Shows activity significantly greater than wild-type. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4296292 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201037 | 1 – 213 | KHG/KDPG aldolaseAdd BLAST | 213 |
Proteomic databases
jPOSTi | P0A955 |
PaxDbi | P0A955 |
PRIDEi | P0A955 |
2D gel databases
SWISS-2DPAGEi | P0A955 |
Expressioni
Inductioni
Constitutive, three-fold induction occurs for growth on gluconate and two-fold for growth on hexuronic acids.1 Publication
Interactioni
Subunit structurei
Homotrimer.
3 PublicationsBinary interactionsi
P0A955
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-558114,EBI-558114 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259153, 29 interactors 850724, 2 interactors |
DIPi | DIP-36196N |
IntActi | P0A955, 10 interactors |
STRINGi | 511145.b1850 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A955 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A955 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 8 – 9 | Substrate binding | 2 |
Sequence similaritiesi
Belongs to the KHG/KDPG aldolase family.Curated
Phylogenomic databases
eggNOGi | COG0800, Bacteria |
HOGENOMi | CLU_077795_1_1_6 |
InParanoidi | P0A955 |
PhylomeDBi | P0A955 |
Family and domain databases
CDDi | cd00452, KDPG_aldolase, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR000887, Aldlse_KDPG_KHG IPR013785, Aldolase_TIM IPR031337, KDPG/KHG_AS_1 IPR031338, KDPG/KHG_AS_2 |
PANTHERi | PTHR30246, PTHR30246, 1 hit |
Pfami | View protein in Pfam PF01081, Aldolase, 1 hit |
TIGRFAMsi | TIGR01182, eda, 1 hit |
PROSITEi | View protein in PROSITE PS00159, ALDOLASE_KDPG_KHG_1, 1 hit PS00160, ALDOLASE_KDPG_KHG_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A955-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNWKTSAES ILTTGPVVPV IVVKKLEHAV PMAKALVAGG VRVLEVTLRT
60 70 80 90 100
ECAVDAIRAI AKEVPEAIVG AGTVLNPQQL AEVTEAGAQF AISPGLTEPL
110 120 130 140 150
LKAATEGTIP LIPGISTVSE LMLGMDYGLK EFKFFPAEAN GGVKALQAIA
160 170 180 190 200
GPFSQVRFCP TGGISPANYR DYLALKSVLC IGGSWLVPAD ALEAGDYDRI
210
TKLAREAVEG AKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68871 Genomic DNA Translation: CAA48732.1 M87458 Genomic DNA Translation: AAA23723.1 L20897 Genomic DNA Translation: AAA23862.1 X63694 Genomic DNA Translation: CAA45222.1 U00096 Genomic DNA Translation: AAC74920.1 AP009048 Genomic DNA Translation: BAA15658.1 |
PIRi | B42986, ADECOG |
RefSeqi | NP_416364.1, NC_000913.3 WP_000800512.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74920; AAC74920; b1850 BAA15658; BAA15658; BAA15658 |
GeneIDi | 58389852 946367 |
KEGGi | ecj:JW1839 eco:b1850 |
PATRICi | fig|1411691.4.peg.399 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68871 Genomic DNA Translation: CAA48732.1 M87458 Genomic DNA Translation: AAA23723.1 L20897 Genomic DNA Translation: AAA23862.1 X63694 Genomic DNA Translation: CAA45222.1 U00096 Genomic DNA Translation: AAC74920.1 AP009048 Genomic DNA Translation: BAA15658.1 |
PIRi | B42986, ADECOG |
RefSeqi | NP_416364.1, NC_000913.3 WP_000800512.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EUA | X-ray | 1.95 | A/B/C | 1-213 | [»] | |
1EUN | X-ray | 2.00 | A/B/C | 1-213 | [»] | |
1FQ0 | X-ray | 2.10 | A/B/C | 1-213 | [»] | |
1FWR | X-ray | 2.70 | A/B/C | 1-213 | [»] | |
1WAU | X-ray | 2.80 | A | 1-213 | [»] | |
1WBH | X-ray | 1.55 | A/B/C | 1-213 | [»] | |
2C0A | X-ray | 1.55 | A/B/C | 1-213 | [»] | |
SMRi | P0A955 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259153, 29 interactors 850724, 2 interactors |
DIPi | DIP-36196N |
IntActi | P0A955, 10 interactors |
STRINGi | 511145.b1850 |
Chemistry databases
ChEMBLi | CHEMBL4296292 |
2D gel databases
SWISS-2DPAGEi | P0A955 |
Proteomic databases
jPOSTi | P0A955 |
PaxDbi | P0A955 |
PRIDEi | P0A955 |
Genome annotation databases
EnsemblBacteriai | AAC74920; AAC74920; b1850 BAA15658; BAA15658; BAA15658 |
GeneIDi | 58389852 946367 |
KEGGi | ecj:JW1839 eco:b1850 |
PATRICi | fig|1411691.4.peg.399 |
Organism-specific databases
EchoBASEi | EB0252 |
Phylogenomic databases
eggNOGi | COG0800, Bacteria |
HOGENOMi | CLU_077795_1_1_6 |
InParanoidi | P0A955 |
PhylomeDBi | P0A955 |
Enzyme and pathway databases
UniPathwayi | UPA00227 UPA00856;UER00829 |
BioCyci | EcoCyc:KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER MetaCyc:KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER |
BRENDAi | 4.1.2.14, 2026 4.1.3.42, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A955 |
PROi | PR:P0A955 |
Family and domain databases
CDDi | cd00452, KDPG_aldolase, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR000887, Aldlse_KDPG_KHG IPR013785, Aldolase_TIM IPR031337, KDPG/KHG_AS_1 IPR031338, KDPG/KHG_AS_2 |
PANTHERi | PTHR30246, PTHR30246, 1 hit |
Pfami | View protein in Pfam PF01081, Aldolase, 1 hit |
TIGRFAMsi | TIGR01182, eda, 1 hit |
PROSITEi | View protein in PROSITE PS00159, ALDOLASE_KDPG_KHG_1, 1 hit PS00160, ALDOLASE_KDPG_KHG_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALKH_ECOLI | |
Accessioni | P0A955Primary (citable) accession number: P0A955 Secondary accession number(s): P10177 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families