UniProtKB - P0A944 (RIMI_ECOLI)
Protein
[Ribosomal protein S18]-alanine N-acetyltransferase
Gene
rimI
Organism
Escherichia coli (strain K12)
Status
Functioni
Acetylates the N-terminal alanine of ribosomal protein S18 (PubMed:6991870, PubMed:2828880). Also acts as a N-epsilon-lysine acetyltransferase that catalyzes acetylation of several proteins (PubMed:30352934).3 Publications
Catalytic activityi
- acetyl-CoA + N-terminal L-alanyl-[ribosomal protein bS18] = CoA + H+ + N-terminal Nα-acetyl-L-alanyl-[ribosomal protein bS18]UniRule annotation1 PublicationEC:2.3.1.266UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 103 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 108 | Acetyl-CoAUniRule annotation | 1 | |
Active sitei | 115 | Proton donorUniRule annotation | 1 |
GO - Molecular functioni
- N-acetyltransferase activity Source: EcoliWiki
- peptide alpha-N-acetyltransferase activity Source: GO_Central
- peptide-lysine-N-acetyltransferase activity Source: EcoCyc
- ribosomal-protein-alanine N-acetyltransferase activity Source: EcoliWiki
- transferase activity, transferring acyl groups other than amino-acyl groups Source: GO_Central
GO - Biological processi
- cellular protein modification process Source: EcoliWiki
- internal peptidyl-lysine acetylation Source: EcoCyc
- N-terminal peptidyl-alanine acetylation Source: EcoliWiki
- N-terminal protein amino acid acetylation Source: EcoliWiki
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10850-MONOMER MetaCyc:EG10850-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rimI1 PublicationUniRule annotation Ordered Locus Names:b4373, JW4335 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotationCurated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutation of the gene impairs the acetylation of the N-terminal alanine of ribosomal protein S18, but does not affect acetylation of ribosomal proteins S5 and L12.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 115 | Y → A: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074561 | 1 – 148 | [Ribosomal protein S18]-alanine N-acetyltransferaseAdd BLAST | 148 |
Proteomic databases
jPOSTi | P0A944 |
PaxDbi | P0A944 |
PRIDEi | P0A944 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262786, 25 interactors 853171, 1 interactor |
DIPi | DIP-48259N |
IntActi | P0A944, 1 interactor |
STRINGi | 511145.b4373 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 147 | N-acetyltransferaseUniRule annotationAdd BLAST | 146 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 69 – 71 | Acetyl-CoA bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0456, Bacteria |
HOGENOMi | CLU_013985_23_2_6 |
InParanoidi | P0A944 |
PhylomeDBi | P0A944 |
Family and domain databases
HAMAPi | MF_02210, RimI, 1 hit |
InterProi | View protein in InterPro IPR006464, AcTrfase_RimI/Ard1 IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR043690, RimI |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR01575, rimI, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A944-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTISSLETT DLPAAYHIEQ RAHAFPWSEK TFASNQGERY LNFQLTQNGK
60 70 80 90 100
MAAFAITQVV LDEATLFNIA VDPDYQRQGL GRALLEHLID ELEKRGVATL
110 120 130 140
WLEVRASNAA AIALYESLGF NEATIRRNYY PTTDGREDAI IMALPISM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 143 – 148 | ALPISM → RCQSVCNTRWNNEVGLDFL in CAA29489 (PubMed:2828880).Curated | 6 | |
Sequence conflicti | 143 – 148 | ALPISM → RCQSVCNTRWNNEVGLDFL in AAA97269 (PubMed:7610040).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06117 Genomic DNA Translation: CAA29489.1 U14003 Genomic DNA Translation: AAA97269.1 U00096 Genomic DNA Translation: AAC77326.1 AP009048 Genomic DNA Translation: BAE78361.1 L05387 Unassigned DNA Translation: AAA03077.1 |
PIRi | D65252 |
RefSeqi | NP_418790.1, NC_000913.3 WP_001092461.1, NZ_SSZK01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC77326; AAC77326; b4373 BAE78361; BAE78361; BAE78361 |
GeneIDi | 49586221 948894 |
KEGGi | ecj:JW4335 eco:b4373 |
PATRICi | fig|1411691.4.peg.2315 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06117 Genomic DNA Translation: CAA29489.1 U14003 Genomic DNA Translation: AAA97269.1 U00096 Genomic DNA Translation: AAC77326.1 AP009048 Genomic DNA Translation: BAE78361.1 L05387 Unassigned DNA Translation: AAA03077.1 |
PIRi | D65252 |
RefSeqi | NP_418790.1, NC_000913.3 WP_001092461.1, NZ_SSZK01000015.1 |
3D structure databases
SMRi | P0A944 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262786, 25 interactors 853171, 1 interactor |
DIPi | DIP-48259N |
IntActi | P0A944, 1 interactor |
STRINGi | 511145.b4373 |
Proteomic databases
jPOSTi | P0A944 |
PaxDbi | P0A944 |
PRIDEi | P0A944 |
Genome annotation databases
EnsemblBacteriai | AAC77326; AAC77326; b4373 BAE78361; BAE78361; BAE78361 |
GeneIDi | 49586221 948894 |
KEGGi | ecj:JW4335 eco:b4373 |
PATRICi | fig|1411691.4.peg.2315 |
Organism-specific databases
EchoBASEi | EB0843 |
Phylogenomic databases
eggNOGi | COG0456, Bacteria |
HOGENOMi | CLU_013985_23_2_6 |
InParanoidi | P0A944 |
PhylomeDBi | P0A944 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10850-MONOMER MetaCyc:EG10850-MONOMER |
Miscellaneous databases
PROi | PR:P0A944 |
Family and domain databases
HAMAPi | MF_02210, RimI, 1 hit |
InterProi | View protein in InterPro IPR006464, AcTrfase_RimI/Ard1 IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR043690, RimI |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR01575, rimI, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIMI_ECOLI | |
Accessioni | P0A944Primary (citable) accession number: P0A944 Secondary accession number(s): P09453, Q2M5U5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families