UniProtKB - P0A935 (MLTA_ECOLI)
Protein
Membrane-bound lytic murein transglycosylase A
Gene
mltA
Organism
Escherichia coli (strain K12)
Status
Functioni
Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi.
Catalytic activityi
- Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue. EC:4.2.2.n1
pH dependencei
Optimum pH is 4.0-4.5.
Temperature dependencei
Loses rapidly its activity at temperatures above 30 degrees Celsius.
GO - Molecular functioni
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
- lyase activity Source: UniProtKB-KW
- lytic transglycosylase activity Source: EcoCyc
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan catabolic process Source: EcoCyc
- peptidoglycan turnover Source: InterPro
Keywordsi
Molecular function | Lyase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | EcoCyc:G7457-MONOMER MetaCyc:G7457-MONOMER |
Protein family/group databases
CAZyi | GH102, Glycoside Hydrolase Family 102 |
Names & Taxonomyi
Protein namesi | Recommended name: Membrane-bound lytic murein transglycosylase A (EC:4.2.2.n1)Alternative name(s): Mlt38 Murein hydrolase A |
Gene namesi | Name:mltA Synonyms:mlt, ygdM Ordered Locus Names:b2813, JW2784 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
Plasma Membrane
- integral component of plasma membrane Source: EcoliWiki
Other locations
- anchored component of cell outer membrane Source: EcoCyc
- cell outer membrane Source: CACAO
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | CuratedAdd BLAST | 20 | |
ChainiPRO_0000032781 | 21 – 365 | Membrane-bound lytic murein transglycosylase AAdd BLAST | 345 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 21 | N-palmitoyl cysteineCurated | 1 | |
Lipidationi | 21 | S-diacylglycerol cysteineCurated | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P0A935 |
PaxDbi | P0A935 |
PRIDEi | P0A935 |
Interactioni
Subunit structurei
Forms a trimeric complex with MrcB/PonB and MipA in vitro.
Protein-protein interaction databases
BioGRIDi | 4261126, 189 interactors |
DIPi | DIP-51238N |
IntActi | P0A935, 2 interactors |
STRINGi | 511145.b2813 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A935 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A935 |
Family & Domainsi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG2821, Bacteria |
HOGENOMi | CLU_037751_2_0_6 |
InParanoidi | P0A935 |
PhylomeDBi | P0A935 |
Family and domain databases
CDDi | cd14485, mltA_like_LT_A, 1 hit |
Gene3Di | 2.40.40.10, 1 hit |
InterProi | View protein in InterPro IPR010611, 3D_dom IPR026044, MltA IPR034654, MltA_3D IPR005300, MltA_B IPR036908, RlpA-like_sf |
PANTHERi | PTHR30124, PTHR30124, 1 hit |
Pfami | View protein in Pfam PF06725, 3D, 1 hit PF03562, MltA, 1 hit |
PIRSFi | PIRSF019422, MltA, 1 hit |
SMARTi | View protein in SMART SM00925, MltA, 1 hit |
SUPFAMi | SSF50685, SSF50685, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A935-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGRWVKYLL MGTVVAMLAA CSSKPTDRGQ QYKDGKFTQP FSLVNQPDAV
60 70 80 90 100
GAPINAGDFA EQINHIRNSS PRLYGNQSNV YNAVQEWLRA GGDTRNMRQF
110 120 130 140 150
GIDAWQMEGA DNYGNVQFTG YYTPVIQARH TRQGEFQYPI YRMPPKRGRL
160 170 180 190 200
PSRAEIYAGA LSDKYILAYS NSLMDNFIMD VQGSGYIDFG DGSPLNFFSY
210 220 230 240 250
AGKNGHAYRS IGKVLIDRGE VKKEDMSMQA IRHWGETHSE AEVRELLEQN
260 270 280 290 300
PSFVFFKPQS FAPVKGASAV PLVGRASVAS DRSIIPPGTT LLAEVPLLDN
310 320 330 340 350
NGKFNGQYEL RLMVALDVGG AIKGQHFDIY QGIGPEAGHR AGWYNHYGRV
360
WVLKTAPGAG NVFSG
Sequence cautioni
The sequence AAB40463 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 346 | H → N in AAC45723 (PubMed:9287002).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32224 Genomic DNA Translation: AAC45723.1 U29581 Genomic DNA Translation: AAB40463.1 Different initiation. U00096 Genomic DNA Translation: AAC75855.1 AP009048 Genomic DNA Translation: BAE76885.1 |
PIRi | A65064 |
RefSeqi | NP_417293.1, NC_000913.3 WP_000678646.1, NZ_STEB01000030.1 |
Genome annotation databases
EnsemblBacteriai | AAC75855; AAC75855; b2813 BAE76885; BAE76885; BAE76885 |
GeneIDi | 57730383 944964 |
KEGGi | ecj:JW2784 eco:b2813 |
PATRICi | fig|1411691.4.peg.3920 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32224 Genomic DNA Translation: AAC45723.1 U29581 Genomic DNA Translation: AAB40463.1 Different initiation. U00096 Genomic DNA Translation: AAC75855.1 AP009048 Genomic DNA Translation: BAE76885.1 |
PIRi | A65064 |
RefSeqi | NP_417293.1, NC_000913.3 WP_000678646.1, NZ_STEB01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AE0 | X-ray | 2.00 | X | 22-365 | [»] | |
2GAE | X-ray | 2.50 | A | 22-365 | [»] | |
2PI8 | X-ray | 2.25 | A/B/C/D | 22-365 | [»] | |
2PIC | X-ray | 2.25 | A | 22-365 | [»] | |
2PJJ | X-ray | 2.46 | A | 22-365 | [»] | |
SMRi | P0A935 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261126, 189 interactors |
DIPi | DIP-51238N |
IntActi | P0A935, 2 interactors |
STRINGi | 511145.b2813 |
Protein family/group databases
CAZyi | GH102, Glycoside Hydrolase Family 102 |
Proteomic databases
jPOSTi | P0A935 |
PaxDbi | P0A935 |
PRIDEi | P0A935 |
Genome annotation databases
EnsemblBacteriai | AAC75855; AAC75855; b2813 BAE76885; BAE76885; BAE76885 |
GeneIDi | 57730383 944964 |
KEGGi | ecj:JW2784 eco:b2813 |
PATRICi | fig|1411691.4.peg.3920 |
Organism-specific databases
EchoBASEi | EB2894 |
Phylogenomic databases
eggNOGi | COG2821, Bacteria |
HOGENOMi | CLU_037751_2_0_6 |
InParanoidi | P0A935 |
PhylomeDBi | P0A935 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7457-MONOMER MetaCyc:G7457-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A935 |
PROi | PR:P0A935 |
Family and domain databases
CDDi | cd14485, mltA_like_LT_A, 1 hit |
Gene3Di | 2.40.40.10, 1 hit |
InterProi | View protein in InterPro IPR010611, 3D_dom IPR026044, MltA IPR034654, MltA_3D IPR005300, MltA_B IPR036908, RlpA-like_sf |
PANTHERi | PTHR30124, PTHR30124, 1 hit |
Pfami | View protein in Pfam PF06725, 3D, 1 hit PF03562, MltA, 1 hit |
PIRSFi | PIRSF019422, MltA, 1 hit |
SMARTi | View protein in SMART SM00925, MltA, 1 hit |
SUPFAMi | SSF50685, SSF50685, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MLTA_ECOLI | |
Accessioni | P0A935Primary (citable) accession number: P0A935 Secondary accession number(s): P46885 Q46928 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references